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Literature summary for 2.7.1.21 extracted from
- Mouse ascites Sarcoma 180 deoxythymidine kinase. General properties and inhibition studies (1974), Biochemistry, 13, 1179-1185.
General Stability
| General Stability | Organism |
|---|---|
| stability decreases with increasing purity, ATP, thymidine, Mg2+ protect | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2'-deoxy-3'-aminothymidine | 5 mM | Mus musculus |  |
| 2-mercaptoethanol | 5 mM | Mus musculus | |
| 5'-aminothymidine | - |
Mus musculus | |
| dTTP | - |
Mus musculus | |
| additional information | 5'-derivatives of thymidine | Mus musculus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.009 | - |
thymidine | pH 7.8, 37°C | Mus musculus | |
| 0.15 | - |
CTP | ATP | Mus musculus | |
| 0.15 | - |
ATP | pH 7.8, 37°C | Mus musculus | |
| 0.3 | - |
ATP | GTP | Mus musculus | |
| 0.3 | - |
GTP | pH 7.8, 37°C | Mus musculus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| sarcoma cell | - |
Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.053 | - |
- |
Mus musculus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 0.1 mM thymidine, 1 week, 30% loss of activity | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + thymidine | ATP can be substituted by GTP | Mus musculus | ADP + dTMP | - |
? | |
| ATP + thymidine | ATP can be substituted by dGTP | Mus musculus | ADP + dTMP | - |
? | |
| ATP + thymidine | ATP can be substituted by ATP-arabinoside | Mus musculus | ADP + dTMP | - |
? | |
| ATP + thymidine | ATP can be substituted by UTP | Mus musculus | ADP + dTMP | - |
? | |
| ATP + thymidine | ATP can be substituted by dCTP | Mus musculus | ADP + dTMP | - |
? | |
| CTP + thymidine | - |
Mus musculus | CDP + thymidine 5'-phosphate | - |
? | |
| GTP + thymidine | - |
Mus musculus | GDP + thymidine 5'-phosphate | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
ATP, thymidine, Mg2+ protect against thermal inactivation | Mus musculus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Mus musculus | |
| 0.003 | - |
2'-deoxy-3'-aminothymidine | pH 7.8, 37°C | Mus musculus | |
| 3 | - |
5'-aminothymidine | pH 7.8, 37°C | Mus musculus | |
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Release 2023.1 (January 2023)
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