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Literature summary for 2.7.1.20 extracted from

  • Song, K.; Li, Y.; He, H.; Liu, L.; Zhao, P.; Xia, Q.; Wang, Y.
    A novel adenosine kinase from Bombyx mori enzymatic activity, structure, and biological function (2019), Int. J. Mol. Sci., 20, 3732 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.20

Cloned(Commentary)

Cloned (Comment) Organism
-
 Bombyx mori

Protein Variants

Protein Variants Comment Organism
E229A complete loss of activity, decrease in the alpha-helix content Bombyx mori
E229R complete loss of activity, significant decrease in the alpha-helix content Bombyx mori
F303A complete loss of activity, decrease in the alpha-helix content Bombyx mori
G280A mutation has no significant influence on the enzymatic activity compared with that of wild-type enzyme, slight decrease in the alpha-helix content Bombyx mori
G280D mutation has no significant influence on the enzymatic activity compared with that of wild-type enzyme, slight decrease in the alpha-helix content Bombyx mori
G68A mutation results in significant decrease in activity Bombyx mori
S201A mutation significantly increases the alpha-helix content of BmADK and its activity Bombyx mori
S201D significant decrease in the alpha-helix content Bombyx mori
S201R significant decrease in the alpha-helix content Bombyx mori

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Bombyx mori 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 gel filtration Bombyx mori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + adenosine Bombyx mori adenosine kinase (ADK) is the first enzyme in the adenosine remediation pathway that catalyzes adenosine phosphorylation into adenosine monophosphate, thus regulating adenosine homeostasis in cells ADP + AMP
-
 ?

Organism

Organism UniProt Comment Textmining
Bombyx mori Q2F635
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bombyx mori

Source Tissue

Source Tissue Comment Organism Textmining
silk gland highly expressed in the silk gland during the pre-pupal stage Bombyx mori
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + adenosine
-
 Bombyx mori ADP + AMP
-
 ?
ATP + adenosine adenosine kinase (ADK) is the first enzyme in the adenosine remediation pathway that catalyzes adenosine phosphorylation into adenosine monophosphate, thus regulating adenosine homeostasis in cells Bombyx mori ADP + AMP
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 38000, SDS-PAGE Bombyx mori

Synonyms

Synonyms Comment Organism
ADK
-
 Bombyx mori

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 stable below Bombyx mori
46.51
-
 melting temperature Bombyx mori

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 11 stable Bombyx mori

pI Value

Organism Comment pI Value Maximum pI Value
Bombyx mori calculated from sequence
-
 5.13

General Information

General Information Comment Organism
metabolism adenosine kinase (ADK) is the first enzyme in the adenosine remediation pathway that catalyzes adenosine phosphorylation into adenosine monophosphate, thus regulating adenosine homeostasis in cells. RNA interference reveals the downregulation of the enzyme (BmADK) decreases ATG-8, caspase-9, Ec-R, E74A, and Br-C expression, indicating it is likely involved in 20E signaling, apoptosis, and autophagy to regulate silk gland degeneration and silkworm metamorphosis Bombyx mori