Literature summary for 2.7.1.20 extracted from

Maj, M.C.; Singh, B.; Gupta, R.S.
Structure-Activity Studies on Mammalian Adenosine Kinase (2000), Biochem. Biophys. Res. Commun., 275, 386-393.

Search for more literature for 2.7.1.20

Protein Variants

Protein Variants	Comment	Organism
additional information	removal of residues 17 to 28 from the N-terminal end of the protein leads to a progressive loss of activity from 100% to 3%. Deletions beyond residue M28 are devoid of activity. Removal of residues at the C-terminal end results in significant loss of activity. Deletions beyond residue R348 are inactive. Site-directed replacement of an aspartic acid redidue D316 leads to complete loss of activity	Cricetulus griseus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	removal of residues 17 to 28 from the N-terminal end of the protein leads to an increase in the Km for adenosine. Removal of the first 10 residues from the C-terminal end causes a 5fold increase in the Km for ATP	Cricetulus griseus

Organism

Organism	UniProt	Comment	Textmining
Cricetulus griseus	-	chinese hamster	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + adenosine	-	Cricetulus griseus	ADP + AMP	-	?

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	removal of residues 17 to 28 from the N-terminal end of the protein leads to an increase in thermal instability	Cricetulus griseus

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Release 2023.1 (January 2023)