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Literature summary for 2.7.1.20 extracted from

  • Yamada, Y.; Goto, H.; Ogasawara, N.
    Purification and properties of adenosine kinase from rat brain (1980), Biochim. Biophys. Acta, 616, 199-207.
    View publication on PubMed

General Stability

General Stability Organism
0.15 M KCl stabilizes, rapid inactivation at low salt concentrations, DTT reverses Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
adenosine above 0.0005 mM Rattus norvegicus
Cu2+
-
Rattus norvegicus
EDTA
-
Rattus norvegicus
Mg2+ above 0.4 mM Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00015 0.0005 adenosine pH 7.5, 30ºC Rattus norvegicus
0.006
-
ATP pH 7.5, 30ºC, with deoxyadenosine as substrate Rattus norvegicus
0.02
-
ATP pH 7.5, 30ºC Rattus norvegicus
0.1
-
MgCl2 pH 7.5, 30ºC Rattus norvegicus
0.25
-
ATP pH 7.5, 30ºC, with deoxyadenosine as substrate Rattus norvegicus
0.36 0.67 2'-deoxy-adenosine pH 7.5, 30ºC Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ no activation Rattus norvegicus
Ca2+ can partially substitute for Mg2+ Rattus norvegicus
Ca2+ activation Rattus norvegicus
Co2+ activation Rattus norvegicus
Co2+ as efective as ATP Rattus norvegicus
Fe2+ no activation Rattus norvegicus
Mg2+ activation Rattus norvegicus
Mn2+ can partially substitute for Mg2+ Rattus norvegicus
Mn2+ activation Rattus norvegicus
additional information no activation by Zn2+ and Ba2+ Rattus norvegicus
additional information no activation by Cr2+ Rattus norvegicus
additional information no activation by Fe2+ Rattus norvegicus
Ni2+ can partially substitute for Mg2+ Rattus norvegicus
Ni2+ activation Rattus norvegicus
Zn2+ no activation Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
gel filtration Rattus norvegicus
41000
-
7.5% acrylamide gel electrophoresis in the presence of SDS Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation, affinity chromatography and gel filtration Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.8
-
-
Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2'-deoxy-adenosine
-
Rattus norvegicus ADP + 2'-deoxy-AMP
-
?
ATP + adenosine
-
Rattus norvegicus ADP + AMP
-
?
GTP + adenosine more effective than ATP Rattus norvegicus GDP + AMP
-
?
UTP + adenosine
-
Rattus norvegicus UDP + AMP
-
?

Subunits

Subunits Comment Organism
monomer 1 * 41000, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
biphasic pH-profile with adenosine, monophasic with deoxyadenosine Rattus norvegicus
5.5
-
sharp, adenosine as substrate Rattus norvegicus
6
-
monophasic, adenosine 0.025 mM Rattus norvegicus
7.5 8.5 broad, adenosine or deoxyadenosine as substrates Rattus norvegicus