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Literature summary for 2.7.1.20 extracted from
- Adenosine kinase from bovine adrenal medulla (1985), Eur. J. Biochem., 151, 365-371.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| adenosine | above 0.002 mM, at pH 7.5 | Bos taurus |  |
| adenosine-5'-pentaphospho-5'-adenosine | competitive with respect to MgATP2- and uncompetitive with respect to adenosine | Bos taurus | |
| adenosine-5'-tetraphospho-5'-adenosine | competitive with respect to ATP and uncompetitive with respect to adenosine | Bos taurus | |
| ADP | product inhibition | Bos taurus | |
| AMP | product inhibition | Bos taurus | |
| ATP | free form | Bos taurus | |
| Bisadenylyl polyphosphates | kinetics | Bos taurus | |
| Cu2+ | - |
Bos taurus | |
| Mg2+ | free ion, strong | Bos taurus | |
| additional information | no inhibition by NaVO3; product inhibition study | Bos taurus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic study | Bos taurus | |
| 0.00015 | 0.0005 | adenosine | pH 7.5, 37ºC | Bos taurus | |
| 0.02 | 0.024 | ATP | pH 7.5, 37ºC | Bos taurus | |
| 0.02 | 0.024 | ATP | ATP in form of MgATP2- | Bos taurus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | activation | Bos taurus | |
| Ca2+ | activation | Bos taurus | |
| Co2+ | activation | Bos taurus | |
| Co2+ | more efficient than Mg2+ | Bos taurus | |
| Mg2+ | requirement | Bos taurus | |
| Mg2+ | reactive species: MgATP2- | Bos taurus | |
| Mn2+ | activation | Bos taurus | |
| Ni2+ | activation | Bos taurus | |
| Ni2+ | more efficient than Mg2+ | Bos taurus | |
| Zn2+ | activation | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
gel filtration | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + adenosine | Bos taurus | key enzyme for the regulation of intracellular adenosine level | ADP + AMP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
male calf | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, gel filtration and affinity chromatography, 1600fold purification | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adrenal medulla | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -40°C, at least 1 year | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2'-deoxy-adenosine | - |
Bos taurus | ADP + 2'-deoxy-AMP | - |
? | |
| ATP + adenosine | specificity | Bos taurus | ADP + AMP | - |
? | |
| ATP + adenosine | ATP-analogues are also substrates | Bos taurus | ADP + AMP | - |
? | |
| ATP + adenosine | broad specificity for purine trinucleotides | Bos taurus | ADP + AMP | - |
? | |
| ATP + adenosine | key enzyme for the regulation of intracellular adenosine level | Bos taurus | ADP + AMP | - |
? | |
| GTP + adenosine | best substrate | Bos taurus | GDP + AMP | - |
? | |
| GTP + adenosine | equally effective as ITP | Bos taurus | GDP + AMP | - |
? | |
| ITP + adenosine | best substrate | Bos taurus | IDP + AMP | - |
? | |
| ITP + adenosine | about half as effective as GTP | Bos taurus | IDP + AMP | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Bos taurus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00006 | - |
adenosine-5'-tetraphospho-5'-adenosine | pH 7.5, 37ºC, with respect to ATP | Bos taurus | |
| 0.0004 | - |
adenosine-5'-tetraphospho-5'-adenosine | pH 7.5, 37ºC, with respect to adenosine | Bos taurus | |
| 0.0004 | - |
adenosine-5'-pentaphospho-5'-adenosine | pH 7.5, 37ºC, with respect to MgATP2- | Bos taurus | |
| 0.002 | - |
adenosine-5'-pentaphospho-5'-adenosine | pH 7.5, 37ºC, with respect to adenosine | Bos taurus | |
| 0.015 | - |
AMP | pH 7.5, 37ºC, with respect to adenosine | Bos taurus | |
| 0.15 | - |
AMP | pH 7.5, 37ºC, with respect to MgATP2- | Bos taurus | |
| 0.2 | - |
ADP | pH 7.5, 37ºC, with respect to adenosine and MgATP2- | Bos taurus | |
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Release 2023.1 (January 2023)
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