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Literature summary for 2.7.1.20 extracted from

  • Yamada, Y.; Goto, H.; Ogasawara, N.
    Adenosine kinase from human liver (1981), Biochim. Biophys. Acta, 660, 36-43.
    View publication on PubMed

General Stability

General Stability Organism
stable when thawed and refrozen Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
adenosine substrate inhibition above 0.0005 mM Homo sapiens
Cu2+
-
Homo sapiens
EDTA
-
Homo sapiens
Mg2+ free ion, strong Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00015 0.0005 adenosine pH 7.5, 30ºC Homo sapiens
0.006
-
ATP pH 7.5, 30ºC, with deoxyadenosine as substrate Homo sapiens
0.006
-
ATP ATP in form of MgATP2- Homo sapiens
0.073 0.0755 ATP ATP/Mg2+ ratio: 1 ton 2 Homo sapiens
0.073 0.0755 ATP ATP in form of MgATP2- Homo sapiens
0.36 0.67 deoxyadenosine
-
Homo sapiens
3.7
-
ribavirin
-
Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ no activation Homo sapiens
Ca2+ can partially substitute for Mg2+ Homo sapiens
Ca2+ activation Homo sapiens
Co2+ activation Homo sapiens
Co2+ more efficient than Mg2+ Homo sapiens
Fe2+ can partially substitute for Mg2+ Homo sapiens
Fe2+ activation Homo sapiens
Mg2+ requirement Homo sapiens
Mg2+ reactive species: MgATP2- Homo sapiens
Mn2+ can partially substitute for Mg2+ Homo sapiens
Mn2+ activation Homo sapiens
additional information no activation by Zn2+ and Ba2+ Homo sapiens
additional information no activation by Cr2+ Homo sapiens
Ni2+ can partially substitute for Mg2+ Homo sapiens
Zn2+ no activation Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
amino acid composition of different species Homo sapiens
38000
-
gel filtration Homo sapiens
40000
-
gel filtration and SDS-PAGE Homo sapiens
41000
-
electrophoresis in 7.5% polyacrylamide gel in the presence of SDS Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation, affinity chromatography, gel filtration and chromatography on DEAE-cellulose Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3
-
-
Homo sapiens

Storage Stability

Storage Stability Organism
-70°C, at least 1 year Homo sapiens
4ºC, dithiothreitol or high concentration of salt, stable. Rapid loss of activity at 4ºC with low concentration of salt, recovered when redialyzed against dithiothreitol Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2'-deoxy-adenosine
-
Homo sapiens ?
-
?
ATP + 2'-deoxy-adenosine
-
Homo sapiens ADP + 2'-deoxy-AMP
-
?
ATP + adenosine specificity Homo sapiens ADP + AMP
-
?
ATP + adenosine pyrimidine nucleoside triphosphates are less efficient than purine derivatives, deoxyribonucleotides less efficient than ribonucleotides Homo sapiens ADP + AMP
-
?
ATP + ribavirin
-
Homo sapiens ADP + ribavirin 5'-phosphate
-
?
GTP + adenosine best substrate Homo sapiens GDP + AMP
-
?
GTP + adenosine more effective than ATP Homo sapiens GDP + AMP
-
?

Subunits

Subunits Comment Organism
monomer 1 * 41000, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
sharp, adenosine as substrate Homo sapiens
7.5 8.5 broad, deoxyadenosine as substrate Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
5 9.3 about half-maximal activity at pH 5 and pH 9.3, about 80% of maximal activity at pH 6.7, adenosine as substrate Homo sapiens
6.5 9.3 about half-maximal activity at pH 6.5 and pH 9.3, deoxyadenosine as substrate Homo sapiens