General Stability | Organism |
---|---|
stable when thawed and refrozen | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
adenosine | substrate inhibition above 0.0005 mM | Homo sapiens | |
Cu2+ | - |
Homo sapiens | |
EDTA | - |
Homo sapiens | |
Mg2+ | free ion, strong | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00015 | 0.0005 | adenosine | pH 7.5, 30ºC | Homo sapiens | |
0.006 | - |
ATP | pH 7.5, 30ºC, with deoxyadenosine as substrate | Homo sapiens | |
0.006 | - |
ATP | ATP in form of MgATP2- | Homo sapiens | |
0.073 | 0.0755 | ATP | ATP/Mg2+ ratio: 1 ton 2 | Homo sapiens | |
0.073 | 0.0755 | ATP | ATP in form of MgATP2- | Homo sapiens | |
0.36 | 0.67 | deoxyadenosine | - |
Homo sapiens | |
3.7 | - |
ribavirin | - |
Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | no activation | Homo sapiens | |
Ca2+ | can partially substitute for Mg2+ | Homo sapiens | |
Ca2+ | activation | Homo sapiens | |
Co2+ | activation | Homo sapiens | |
Co2+ | more efficient than Mg2+ | Homo sapiens | |
Fe2+ | can partially substitute for Mg2+ | Homo sapiens | |
Fe2+ | activation | Homo sapiens | |
Mg2+ | requirement | Homo sapiens | |
Mg2+ | reactive species: MgATP2- | Homo sapiens | |
Mn2+ | can partially substitute for Mg2+ | Homo sapiens | |
Mn2+ | activation | Homo sapiens | |
additional information | no activation by Zn2+ and Ba2+ | Homo sapiens | |
additional information | no activation by Cr2+ | Homo sapiens | |
Ni2+ | can partially substitute for Mg2+ | Homo sapiens | |
Zn2+ | no activation | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
amino acid composition of different species | Homo sapiens |
38000 | - |
gel filtration | Homo sapiens |
40000 | - |
gel filtration and SDS-PAGE | Homo sapiens |
41000 | - |
electrophoresis in 7.5% polyacrylamide gel in the presence of SDS | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate fractionation, affinity chromatography, gel filtration and chromatography on DEAE-cellulose | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.3 | - |
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
-70°C, at least 1 year | Homo sapiens |
4ºC, dithiothreitol or high concentration of salt, stable. Rapid loss of activity at 4ºC with low concentration of salt, recovered when redialyzed against dithiothreitol | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2'-deoxy-adenosine | - |
Homo sapiens | ? | - |
? | |
ATP + 2'-deoxy-adenosine | - |
Homo sapiens | ADP + 2'-deoxy-AMP | - |
? | |
ATP + adenosine | specificity | Homo sapiens | ADP + AMP | - |
? | |
ATP + adenosine | pyrimidine nucleoside triphosphates are less efficient than purine derivatives, deoxyribonucleotides less efficient than ribonucleotides | Homo sapiens | ADP + AMP | - |
? | |
ATP + ribavirin | - |
Homo sapiens | ADP + ribavirin 5'-phosphate | - |
? | |
GTP + adenosine | best substrate | Homo sapiens | GDP + AMP | - |
? | |
GTP + adenosine | more effective than ATP | Homo sapiens | GDP + AMP | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 41000, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
sharp, adenosine as substrate | Homo sapiens |
7.5 | 8.5 | broad, deoxyadenosine as substrate | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9.3 | about half-maximal activity at pH 5 and pH 9.3, about 80% of maximal activity at pH 6.7, adenosine as substrate | Homo sapiens |
6.5 | 9.3 | about half-maximal activity at pH 6.5 and pH 9.3, deoxyadenosine as substrate | Homo sapiens |