Literature summary for 2.7.1.20 extracted from

Palella, T.D.; Andres, C.M.; Fox, I.H.
Human placental adenosine kinase. Kinetic mechanism and inhibition (1980), J. Biol. Chem., 255, 5264-5269.

Search for more literature for 2.7.1.20

General Stability

General Stability	Organism
bovine serum albumin stabilizes	Homo sapiens
purified enzyme preparation is stable in the concentrated form	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
2',3'-isopropylidene adenosine	weak inhibition	Homo sapiens
2'-deoxyadenosine	substrate inhibition	Homo sapiens
3'-deoxyadenosine	-	Homo sapiens
4-amino-5-iodo-7beta-D-ribofuranosyl-7H-pyrrole(2,3-d)-pyrimidine	100% inhibition	Homo sapiens
5'-deoxyadenosine	100% inhibition	Homo sapiens
6-gamma,gamma-dimethylallylaminopurine riboside	90% inhibition	Homo sapiens
6-Methylmercaptopurine riboside	strong inhibition, substrate inhibition	Homo sapiens
adenosine	above 0.0025 mM, at pH 7.4, with ATP and Mg2+ 0.2 mM	Homo sapiens
ADP	noncompetitive with respect to adenosine and ATP	Homo sapiens
AMP	competitive with respect to adenosine and noncompetitive with respect to ATP	Homo sapiens
ATP	-	Homo sapiens
deoxymethylthioadenosine	weak inhibition	Homo sapiens
Mg2+	at high concentrations	Homo sapiens
additional information	at pH 5.5, with equimolar concentrations of ATP and Mg2+ from 0.2 to 1 mM there is no inhibition by adenosine over a range of 0 to 0.02 mM; product inhibition study	Homo sapiens
S-adenosylhomocysteine	97% inhibition	Homo sapiens
uridine	weak inhibition	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00015	0.0005	adenosine	pH 7.4, 37ºC	Homo sapiens
0.073	0.0755	ATP	pH 7.4, 37ºC	Homo sapiens
0.073	0.0755	ATP	ATP in form of MgATP2-	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	requirement	Homo sapiens
Mg2+	reactive species: MgATP2-	Homo sapiens
Mg2+	optimum activity with 0.4-0.8 mM, at pH 6.5. Above, inhibition	Homo sapiens
Mg2+	free nucleotide or free metal ion exert inhibitory effects	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
3600fold purification	Homo sapiens

Reaction

Reaction	Comment	Organism	Reaction ID
ATP + adenosine = ADP + AMP	mechanism	Homo sapiens	a
ATP + adenosine = ADP + AMP	ordered bi bi mechanism	Homo sapiens	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
placenta	-	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.8	-	-	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 2'-deoxy-adenosine	-	Homo sapiens	ADP + 2'-deoxy-AMP	-	?
ATP + adenosine	MgATP2- is the true substrate	Homo sapiens	ADP + AMP	-	?

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	Mg2+/ATP ratio 1 to 2	Homo sapiens

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
additional information	-	progressive decrease in activity at pH 7.5 and 8.5, no activity at pH 4.5	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.14	-	AMP	pH 7.4, 37ºC, at variable adenosine and saturating MgATP2- concentration	Homo sapiens
0.5	-	AMP	pH 7.4, 37ºC, at variable ATP and saturating adenosine concentration	Homo sapiens

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Release 2023.1 (January 2023)