Activating Compound | Comment | Organism | Structure |
---|---|---|---|
AMP | 0.5 M, 1.1fold activation | Methylomonas methanica | |
fructose-6-phosphate | 5 mM, 1.16fold activation | Methylomonas methanica | |
pyruvate | 5 mM, 1.15fold activation | Methylomonas methanica |
Cloned (Comment) | Organism |
---|---|
heterologous expression in Escherichia coli | Methylomonas methanica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | 0.5 M, 51% loss of activity | Methylomonas methanica | |
citrate | 1 M, 23% loss of activity | Methylomonas methanica | |
KH2PO4 | 3 M, 14% loss of activity | Methylomonas methanica | |
NaCl | 0.5 M, 50% loss of activity | Methylomonas methanica | |
Pyrophosphate | 2 M, 18% loss of activity | Methylomonas methanica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.078 | - |
ATP | 30°C, pH 7.5 | Methylomonas methanica | |
0.08 | - |
D-glucose | 30°C, pH 7.5 | Methylomonas methanica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | 11% of the activity compared to Mg2+ | Methylomonas methanica | |
Co2+ | 18% of the activity compared to Mg2+ | Methylomonas methanica | |
Cs+ | 52% of the activity compared to Mg2+ | Methylomonas methanica | |
Cu2+ | 3% of the activity compared to Mg2+ | Methylomonas methanica | |
Li+ | 72% of the activity compared to Mg2+ | Methylomonas methanica | |
Mg2+ | highest activity is observed in the presence of 5 mM of MgCl2 | Methylomonas methanica | |
Mn2+ | 60% of the activity compared to Mg2+ | Methylomonas methanica | |
Na2MoO4 | 66% of the activity compared to Mg2+ | Methylomonas methanica | |
Ni2+ | 13% of the activity compared to Mg2+ | Methylomonas methanica | |
Rb+ | 73% of the activity compared to Mg2+ | Methylomonas methanica | |
Sr2+ | 30% of the activity compared to Mg2+ | Methylomonas methanica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35500 | - |
SDS-PAGE | Methylomonas methanica |
71000 | - |
gradient electrophoresis, non-denaturation conditions, dimer | Methylomonas methanica |
106500 | - |
gradient electrophoresis, non-denaturation conditions, trimer | Methylomonas methanica |
142000 | - |
gradient electrophoresis, non-denaturation conditions, tetramer | Methylomonas methanica |
177500 | - |
gradient electrophoresis, non-denaturation conditions, pentamer | Methylomonas methanica |
213000 | - |
gradient electrophoresis, non-denaturation conditions, hexamer | Methylomonas methanica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylomonas methanica | A0A3G4RI75 | - |
- |
Methylomonas methanica 12 | A0A3G4RI75 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Methylomonas methanica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucosamine | - |
Methylomonas methanica | ADP + D-glucosamine 6-phosphate | - |
? | |
ATP + D-glucosamine | - |
Methylomonas methanica 12 | ADP + D-glucosamine 6-phosphate | - |
? | |
ATP + D-glucose | no activity with CTP, ADP, PPi or polyP | Methylomonas methanica | ADP + D-glucose 6-phosphate | - |
? | |
ATP + D-glucose | no activity with CTP, ADP, PPi or polyP | Methylomonas methanica 12 | ADP + D-glucose 6-phosphate | - |
? | |
GTP + D-glucose | 71% compared to the activity with ATP | Methylomonas methanica | GDP + D-glucose 6-phosphate | - |
? | |
GTP + D-glucose | 71% compared to the activity with ATP | Methylomonas methanica 12 | GDP + D-glucose 6-phosphate | - |
? | |
UTP + D-glucose | 85% compared to the activity with ATP | Methylomonas methanica | UDP + D-glucose 6-phosphate | - |
? | |
UTP + D-glucose | 85% compared to the activity with ATP | Methylomonas methanica 12 | UDP + D-glucose 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35500, SDS-PAGE, multimeric protein consisting of di-, tri-, tetra-, penta- and hexamers | Methylomonas methanica |
Synonyms | Comment | Organism |
---|---|---|
ATP-dependent glucokinase | - |
Methylomonas methanica |
ATP-GLK | - |
Methylomonas methanica |
glk | - |
Methylomonas methanica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Methylomonas methanica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
5 min, complete inactivation | Methylomonas methanica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1095 | - |
D-glucose | 30°C, pH 7.5 | Methylomonas methanica | |
0.126 | - |
ATP | 30°C, pH 7.5 | Methylomonas methanica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Methylomonas methanica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9.5 | active in a wide range pH 5 to 9.5, sharp loss of activity above pH 9.5 | Methylomonas methanica |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.37 | - |
D-glucose | 30°C, pH 7.5 | Methylomonas methanica | |
1.61 | - |
ATP | 30°C, pH 7.5 | Methylomonas methanica |