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Literature summary for 2.7.1.2 extracted from

  • Reshetnikov, A.; Solntseva, N.; Rozova, O.; Mustakhimov, I.; Trotsenko, Y.; Khmelenina, V.
    ATP-and polyphosphate-dependent glucokinases from aerobic methanotrophs (2019), Microorganisms, 7, 52 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
AMP 0.5 M, 1.1fold activation Methylomonas methanica
fructose-6-phosphate 5 mM, 1.16fold activation Methylomonas methanica
pyruvate 5 mM, 1.15fold activation Methylomonas methanica

Cloned(Commentary)

Cloned (Comment) Organism
heterologous expression in Escherichia coli Methylomonas methanica

Inhibitors

Inhibitors Comment Organism Structure
ADP 0.5 M, 51% loss of activity Methylomonas methanica
citrate 1 M, 23% loss of activity Methylomonas methanica
KH2PO4 3 M, 14% loss of activity Methylomonas methanica
NaCl 0.5 M, 50% loss of activity Methylomonas methanica
Pyrophosphate 2 M, 18% loss of activity Methylomonas methanica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.078
-
ATP 30°C, pH 7.5 Methylomonas methanica
0.08
-
D-glucose 30°C, pH 7.5 Methylomonas methanica

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ 11% of the activity compared to Mg2+ Methylomonas methanica
Co2+ 18% of the activity compared to Mg2+ Methylomonas methanica
Cs+ 52% of the activity compared to Mg2+ Methylomonas methanica
Cu2+ 3% of the activity compared to Mg2+ Methylomonas methanica
Li+ 72% of the activity compared to Mg2+ Methylomonas methanica
Mg2+ highest activity is observed in the presence of 5 mM of MgCl2 Methylomonas methanica
Mn2+ 60% of the activity compared to Mg2+ Methylomonas methanica
Na2MoO4 66% of the activity compared to Mg2+ Methylomonas methanica
Ni2+ 13% of the activity compared to Mg2+ Methylomonas methanica
Rb+ 73% of the activity compared to Mg2+ Methylomonas methanica
Sr2+ 30% of the activity compared to Mg2+ Methylomonas methanica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35500
-
SDS-PAGE Methylomonas methanica
71000
-
gradient electrophoresis, non-denaturation conditions, dimer Methylomonas methanica
106500
-
gradient electrophoresis, non-denaturation conditions, trimer Methylomonas methanica
142000
-
gradient electrophoresis, non-denaturation conditions, tetramer Methylomonas methanica
177500
-
gradient electrophoresis, non-denaturation conditions, pentamer Methylomonas methanica
213000
-
gradient electrophoresis, non-denaturation conditions, hexamer Methylomonas methanica

Organism

Organism UniProt Comment Textmining
Methylomonas methanica A0A3G4RI75
-
-
Methylomonas methanica 12 A0A3G4RI75
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Methylomonas methanica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-glucosamine
-
Methylomonas methanica ADP + D-glucosamine 6-phosphate
-
?
ATP + D-glucosamine
-
Methylomonas methanica 12 ADP + D-glucosamine 6-phosphate
-
?
ATP + D-glucose no activity with CTP, ADP, PPi or polyP Methylomonas methanica ADP + D-glucose 6-phosphate
-
?
ATP + D-glucose no activity with CTP, ADP, PPi or polyP Methylomonas methanica 12 ADP + D-glucose 6-phosphate
-
?
GTP + D-glucose 71% compared to the activity with ATP Methylomonas methanica GDP + D-glucose 6-phosphate
-
?
GTP + D-glucose 71% compared to the activity with ATP Methylomonas methanica 12 GDP + D-glucose 6-phosphate
-
?
UTP + D-glucose 85% compared to the activity with ATP Methylomonas methanica UDP + D-glucose 6-phosphate
-
?
UTP + D-glucose 85% compared to the activity with ATP Methylomonas methanica 12 UDP + D-glucose 6-phosphate
-
?

Subunits

Subunits Comment Organism
? x * 35500, SDS-PAGE, multimeric protein consisting of di-, tri-, tetra-, penta- and hexamers Methylomonas methanica

Synonyms

Synonyms Comment Organism
ATP-dependent glucokinase
-
Methylomonas methanica
ATP-GLK
-
Methylomonas methanica
glk
-
Methylomonas methanica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Methylomonas methanica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
5 min, complete inactivation Methylomonas methanica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1095
-
D-glucose 30°C, pH 7.5 Methylomonas methanica
0.126
-
ATP 30°C, pH 7.5 Methylomonas methanica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Methylomonas methanica

pH Range

pH Minimum pH Maximum Comment Organism
5 9.5 active in a wide range pH 5 to 9.5, sharp loss of activity above pH 9.5 Methylomonas methanica

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.37
-
D-glucose 30°C, pH 7.5 Methylomonas methanica
1.61
-
ATP 30°C, pH 7.5 Methylomonas methanica