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Literature summary for 2.7.1.2 extracted from

  • Mustakhimov, I.I.; Rozova, O.N.; Solntseva, N.P.; Khmelenina, V.N.; Reshetnikov, A.S.; Trotsenko, Y.A.
    The properties and potential metabolic role of glucokinase in halotolerant obligate methanotroph Methylomicrobium alcaliphilum 20Z (2017), Antonie van Leeuwenhoek, 110, 375-386 .
    View publication on PubMed
Search for more literature for 2.7.1.2

Cloned(Commentary)

Cloned (Comment) Organism
heterologous expression in Escherichia coli BL21 (DE3) as a C-terminal His6 version in an IPTG-inducible expression vector Methylotuvimicrobium alcaliphilum

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
 Methylotuvimicrobium alcaliphilum
KCl 0.2 M, 50% loss of activity Methylotuvimicrobium alcaliphilum
additional information not affected by glucose 6-phosphate Methylotuvimicrobium alcaliphilum
NaCl 0.8 M, 50% loss of activity Methylotuvimicrobium alcaliphilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.32
-
 D-glucose pH 9.5, 30°C Methylotuvimicrobium alcaliphilum
0.37
-
 ATP pH 9.5, 30°C Methylotuvimicrobium alcaliphilum
1.1
-
 D-glucosamine pH 9.5, 30°C Methylotuvimicrobium alcaliphilum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the enzyme is Mg2+- or Mn2+-dependent. KM: 0.38 mM Methylotuvimicrobium alcaliphilum
Mn2+ the enzyme is Mg2+- or Mn2+-dependent. KM: 0.4 mM Methylotuvimicrobium alcaliphilum
additional information CuCl2, CoCl2, ZnCl2 or CaCl2 have no appreciable effect on the enzyme activity Methylotuvimicrobium alcaliphilum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 gradient electrophoresis Methylotuvimicrobium alcaliphilum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-glucose Methylotuvimicrobium alcaliphilum
-
 ADP + D-glucose 6-phosphate
-
 ?
ATP + D-glucose Methylotuvimicrobium alcaliphilum 20Z
-
 ADP + D-glucose 6-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Methylotuvimicrobium alcaliphilum G4T1H4
-
-
Methylotuvimicrobium alcaliphilum 20Z G4T1H4
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methylotuvimicrobium alcaliphilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-glucosamine D-glucose and D-glucosamine with nearly equal activity Methylotuvimicrobium alcaliphilum ADP + D-glucosamine 6-phosphate
-
 ?
ATP + D-glucosamine D-glucose and D-glucosamine with nearly equal activity Methylotuvimicrobium alcaliphilum 20Z ADP + D-glucosamine 6-phosphate
-
 ?
ATP + D-glucose
-
 Methylotuvimicrobium alcaliphilum ADP + D-glucose 6-phosphate
-
 ?
ATP + D-glucose D-glucose and D-glucosamine with nearly equal activity Methylotuvimicrobium alcaliphilum ADP + D-glucose 6-phosphate
-
 ?
ATP + D-glucose
-
 Methylotuvimicrobium alcaliphilum 20Z ADP + D-glucose 6-phosphate
-
 ?
ATP + D-glucose D-glucose and D-glucosamine with nearly equal activity Methylotuvimicrobium alcaliphilum 20Z ADP + D-glucose 6-phosphate
-
 ?
additional information fructose, xylose, galactose, sucrose, lactose, glucose-1-phosphate, glucose-6-phosphate, fructose-1-phosphate or fructose-6-phosphate as a phosphate acceptor. GTP, CTP, UTP, ADP or PPi does not serve as a phosphoryl donor Methylotuvimicrobium alcaliphilum ?
-
-
additional information fructose, xylose, galactose, sucrose, lactose, glucose-1-phosphate, glucose-6-phosphate, fructose-1-phosphate or fructose-6-phosphate as a phosphate acceptor. GTP, CTP, UTP, ADP or PPi does not serve as a phosphoryl donor Methylotuvimicrobium alcaliphilum 20Z ?
-
-

Subunits

Subunits Comment Organism
homodimer 2 * 35000, SDS-PAGE Methylotuvimicrobium alcaliphilum

Synonyms

Synonyms Comment Organism
glk
-
 Methylotuvimicrobium alcaliphilum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Methylotuvimicrobium alcaliphilum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
252.8
-
 ATP pH 9.5, 30°C Methylotuvimicrobium alcaliphilum
257.7
-
 D-glucose pH 9.5, 30°C Methylotuvimicrobium alcaliphilum
293.5
-
 D-glucosamine pH 9.5, 30°C Methylotuvimicrobium alcaliphilum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
-
 Methylotuvimicrobium alcaliphilum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.34
-
 ADP pH 9.5, 30°C Methylotuvimicrobium alcaliphilum

General Information

General Information Comment Organism
malfunction chromosomal deletion of the glk gene resulted in a loss of glucokinase activity and retardation of growth as well as in a decrease of intracellular glycogen content Methylotuvimicrobium alcaliphilum
metabolism the enzyme implicated in the regulation of glycogen biosynthesis and degradation in an obligate methanotroph Methylotuvimicrobium alcaliphilum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
266.8
-
 D-glucosamine pH 9.5, 30°C Methylotuvimicrobium alcaliphilum
683.2
-
 ATP pH 9.5, 30°C Methylotuvimicrobium alcaliphilum
805.3
-
 D-glucose pH 9.5, 30°C Methylotuvimicrobium alcaliphilum