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Literature summary for 2.7.1.2 extracted from

  • Milanes, J.E.; Suryadi, J.; Abendroth, J.; Van Voorhis, W.C.; Barrett, K.F.; Dranow, D.M.; Phan, I.Q.; Patrick, S.L.; Rozema, S.D.; Khalifa, M.M.; Golden, J.E.; Morris, J.C.
    Enzymatic and structural characterization of the Naegleria fowleri glucokinase (2019), Antimicrob. Agents Chemother., 63, e02410-18 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Naegleria fowleri

Crystallization (Commentary)

Crystallization (Comment) Organism
the structure is determined and refined to 2.2 A resolution Naegleria fowleri

Inhibitors

Inhibitors Comment Organism Structure
4-(4-tert-butylbenzamido)-3',4',6-trichloro[1,1'-biphenyl]-3-carboxylic acid
-
 Naegleria fowleri
4-(4-tert-butylbenzamido)-3',6-dichloro-4'-fluoro[1,1'-biphenyl]-3-carboxylic acid
-
 Naegleria fowleri
4-(4-tert-butylbenzamido)-3',6-dichloro[1,1'-biphenyl]-3-carboxylic acid
-
 Naegleria fowleri
4-(4-tert-butylbenzamido)-6-chloro-3'-nitro[1,1'-biphenyl]-3-carboxylic acid
-
 Naegleria fowleri
4-(4-tert-butylbenzamido)-6-chloro-4'-(trifluoromethoxy)[1,1'-biphenyl]-3-carboxylic acid
-
 Naegleria fowleri
4-(4-tert-butylbenzamido)-6-chloro-4'-(trifluoromethyl)[1,1'-biphenyl]-3-carboxylic acid
-
 Naegleria fowleri
ADP
-
 Naegleria fowleri
ebselen
-
 Naegleria fowleri
glucosamine
-
 Naegleria fowleri
N,N'-[furan-2,5-diylbis(2-chloro-4,1-phenylene)]diguanidine
-
 Naegleria fowleri
N,N'-[furan-2,5-diylbis(3-chloro-4,1-phenylene)]diguanidine
-
 Naegleria fowleri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0425
-
 D-glucose pH 7.4, 22°C Naegleria fowleri
0.1416
-
 ATP pH 7.4, 22°C Naegleria fowleri

Metals/Ions

Metals/Ions Comment Organism Structure
CaCl2 MnCl2 yields 9% of the activity observed when MgCl2 is included in the assay Naegleria fowleri
MgCl2 the enzyme functions best when MgCl2 is included in the assay Naegleria fowleri
MnCl2 MnCl2 yields 40% of the activity observed when MgCl2 is included in the assay Naegleria fowleri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-glucose Naegleria fowleri
-
 ADP + D-glucose 6-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Naegleria fowleri A0A384E136
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Naegleria fowleri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-glucosamine
-
 Naegleria fowleri ADP + D-glucosamine 6-phosphate
-
 ?
ATP + D-glucose
-
 Naegleria fowleri ADP + D-glucose 6-phosphate
-
 ?
ATP + D-glucose limited hexose substrate range, with the greatest activity observed with glucose Naegleria fowleri ADP + D-glucose 6-phosphate
-
 ?

Synonyms

Synonyms Comment Organism
NfGlck
-
 Naegleria fowleri

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
36.5
-
 ATP pH 7.4, 22°C Naegleria fowleri
36.5
-
 D-glucose pH 7.4, 22°C Naegleria fowleri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Naegleria fowleri

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9.5 pH 7.5: about 65% of maximal activity, pH 9.5: about 30% of maximal activity Naegleria fowleri

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.116
-
 ADP pH 7.4, 22°C Naegleria fowleri
0.1864
-
 glucosamine pH 7.4, 22°C Naegleria fowleri

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0007
-
 pH and temperature not specified in the publication Naegleria fowleri N,N'-[furan-2,5-diylbis(2-chloro-4,1-phenylene)]diguanidine
0.00088
-
 pH and temperature not specified in the publication Naegleria fowleri ebselen
0.002
-
 pH and temperature not specified in the publication Naegleria fowleri N,N'-[furan-2,5-diylbis(3-chloro-4,1-phenylene)]diguanidine
0.0029
-
 pH and temperature not specified in the publication Naegleria fowleri 4-(4-tert-butylbenzamido)-3',4',6-trichloro[1,1'-biphenyl]-3-carboxylic acid
0.005
-
 pH and temperature not specified in the publication Naegleria fowleri 4-(4-tert-butylbenzamido)-6-chloro-4'-(trifluoromethoxy)[1,1'-biphenyl]-3-carboxylic acid
0.0059
-
 pH and temperature not specified in the publication Naegleria fowleri 4-(4-tert-butylbenzamido)-3',6-dichloro-4'-fluoro[1,1'-biphenyl]-3-carboxylic acid
0.006
-
 pH and temperature not specified in the publication Naegleria fowleri 4-(4-tert-butylbenzamido)-6-chloro-4'-(trifluoromethyl)[1,1'-biphenyl]-3-carboxylic acid
0.0065
-
 pH and temperature not specified in the publication Naegleria fowleri 4-(4-tert-butylbenzamido)-3',6-dichloro[1,1'-biphenyl]-3-carboxylic acid
0.0093
-
 pH and temperature not specified in the publication Naegleria fowleri 4-(4-tert-butylbenzamido)-6-chloro-3'-nitro[1,1'-biphenyl]-3-carboxylic acid

General Information

General Information Comment Organism
metabolism the enzyme plays a role in metabolism of nutrients acquired from the environment through either predation or directly from the sediment Naegleria fowleri