Cloned (Comment) | Organism |
---|---|
gene TM 1469 or glk, functional expression of the soluble His-tagged in Escherichia coli strain BL21(DE3) | Thermotoga maritima |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Thermotoga maritima | |
Fe2+ | - |
Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | activation energy, kinetics | Thermotoga maritima | |
0.36 | - |
ATP | recombinant enyme, pH 7.3, 80°C | Thermotoga maritima | |
1 | - |
D-glucose | recombinant enyme, pH 7.3, 80°C | Thermotoga maritima |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 50% of the activity with Mg2+ | Thermotoga maritima | |
Mg2+ | most efficient divalent cation | Thermotoga maritima | |
Mn2+ | 90% of the activity with Mg2+ | Thermotoga maritima | |
Ni2+ | 20% of the activity with Mg2+ | Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
2 * 36000, alpha2, recombinant enzyme, SDS-PAGE | Thermotoga maritima |
75000 | - |
recombinant enzyme, gel filtration | Thermotoga maritima |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-glucose | Thermotoga maritima | the enzyme catalyzes the first step of the Embden-Meyerhoff pathway and the Entner-Douderoff pathway | ADP + D-glucose 6-phosphate | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
gene TM 1469 or glk | - |
Purification (Comment) | Organism |
---|---|
recombinant soluble His-tagged enzyme from Escherichia coli strain BL21(DE3) by ultracentrifugation, nickel affinity chromatography, and gel filtration to homogeneity | Thermotoga maritima |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
370 | - |
purified recombinant enzyme, Vmax | Thermotoga maritima |
Storage Stability | Organism |
---|---|
-20°C, purified recombinant enzyme, 50 mM Tris-HCl, pH 7.5, stable | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-deoxy-D-glucose | 26% of the activity with D-glucose | Thermotoga maritima | ADP + 2-deoxy-D-glucose 6-phosphate | - |
ir | |
ATP + D-glucose | the enzyme catalyzes the first step of the Embden-Meyerhoff pathway and the Entner-Douderoff pathway | Thermotoga maritima | ADP + D-glucose 6-phosphate | - |
ir | |
ATP + D-glucose | the enzyme is specific for D-glucose | Thermotoga maritima | ADP + D-glucose 6-phosphate | - |
ir | |
CTP + D-glucose | 5% of the activity with ATP as phosphoryl donor | Thermotoga maritima | CDP + D-glucose 6-phosphate | - |
? | |
GTP + D-glucose | 12% of the activity with ATP as phosphoryl donor | Thermotoga maritima | GDP + D-glucose 6-phosphate | - |
? | |
ITP + D-glucose | 20% of the activity with ATP as phosphoryl donor | Thermotoga maritima | IDP + D-glucose 6-phosphate | - |
? | |
additional information | no activity with D-mannose and D-fructose as phosphoryl acceptors, no activity with UDP, ADP, acetyl phosphate, and diphosphate as phosphoryl donors | Thermotoga maritima | ? | - |
? | |
UTP + D-glucose | 12% of the activity with ATP as phosphoryl donor | Thermotoga maritima | UDP + D-glucose 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 36000, alpha2, recombinant enzyme, SDS-PAGE | Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
ATP-GLK | - |
Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
93 | - |
- |
Thermotoga maritima |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
38 | 98 | below 10% of maximal activity at 38°C, about 90% of maximal activity at 98°C | Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
quite stable for 120 min | Thermotoga maritima |
100 | - |
almost complete inactivation after 5 min | Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
- |
Thermotoga maritima |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.8 | 7.7 | 50% activity at pH 6.8 and pH 7.7 | Thermotoga maritima |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | dependent on | Thermotoga maritima | |
CTP | 5% of the activity with ATP | Thermotoga maritima | |
GTP | 12% of the activity with ATP | Thermotoga maritima | |
ITP | 20% of the activity with ATP | Thermotoga maritima | |
UTP | 12% of the activity with ATP | Thermotoga maritima |