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Literature summary for 2.7.1.2 extracted from
- Purification and kinetic characterization of a specific glucokinase from Streptococcus mutans OMZ70 cells (1982), Biochim. Biophys. Acta, 709, 178-186.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | noncompetitive to ATP and uncompetitive to glucose | Streptococcus mutans |  |
| CTP | in presence of ATP | Streptococcus mutans | |
| D-glucose 6-phosphate | - |
Streptococcus mutans | |
| GTP | in presence of ATP | Streptococcus mutans | |
| ITP | in presence of ATP | Streptococcus mutans | |
| TTP | in presence of ATP | Streptococcus mutans | |
| UTP | in presence of ATP | Streptococcus mutans | |
| XTP | in presence of ATP | Streptococcus mutans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.21 | - |
ATP | 22°C, pH 7.5 | Streptococcus mutans | |
| 0.61 | - |
D-glucose | 22°C, pH 7.5 | Streptococcus mutans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 34% of the activation with Mg2+ | Streptococcus mutans | |
| Co2+ | more efficient than Mg2+ in activation, relative rate 264% | Streptococcus mutans | |
| Fe2+ | 48% of the activation with Mg2+ | Streptococcus mutans | |
| Mg2+ | less efficient in activation than Mn2+ or Co2+ | Streptococcus mutans | |
| Mn2+ | more efficient than Mg2+ in activation, relative rate 173% | Streptococcus mutans | |
| Zn2+ | 56% of the activation with Mg2+ | Streptococcus mutans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
alpha2, 2 * 24000, SDS-PAGE | Streptococcus mutans |
| 41000 | - |
gel filtration | Streptococcus mutans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-glucose | Streptococcus mutans | - |
ADP + D-glucose 6-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptococcus mutans | - |
OMZ70, ATCC 33535 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptococcus mutans |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + D-glucose = ADP + D-glucose 6-phosphate | ordered mechanism in which glucose binds first and glucose 6-phosphate dissociates last | Streptococcus mutans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 198 | - |
- |
Streptococcus mutans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-glucose | - |
Streptococcus mutans | ADP + D-glucose 6-phosphate | - |
? | |
| ATP + D-glucose | highly specific for D-glucose | Streptococcus mutans | ADP + D-glucose 6-phosphate | - |
? | |
| CTP + D-glucose | less than 10% of the activity with ATP | Streptococcus mutans | CDP + D-glucose 6-phosphate | - |
? | |
| GTP + D-glucose | less than 10% of ATP | Streptococcus mutans | GDP + D-glucose 6-phosphate | - |
? | |
| ITP + D-glucose | less than 10% of the activity with ATP | Streptococcus mutans | IDP + D-glucose 6-phosphate | - |
? | |
| additional information | less than 10% of the activity with ATP: TTP | Streptococcus mutans | ? | - |
? | |
| UTP + D-glucose | less than 10% of the activity with ATP | Streptococcus mutans | UDP + D-glucose 6-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | alpha2, 2 * 24000, SDS-PAGE | Streptococcus mutans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at | Streptococcus mutans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9.5 | - |
Streptococcus mutans |
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