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Literature summary for 2.7.1.197 extracted from
- Mannitol-specific enzyme II of the bacterial phosphotransferase system. I. Properties of the purified permease (1983), J. Biol. Chem., 258, 10748-10756.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the purified enzyme is dependent on lubrol PX and phospholipid for maximal activity | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-fructose 1,6-bisphosphate | 34% inhibition at 20 mM | Escherichia coli |  |
| D-glucitol | - |
Escherichia coli | |
| D-glucose 1-phosphate | - |
Escherichia coli | |
| D-glucose 6-phosphate | - |
Escherichia coli | |
| D-mannitol | strong substrate inhibition at pH 7.0 | Escherichia coli | |
| D-mannitol 1-phosphate | strong substrate inhibition at pH 7.0 | Escherichia coli | |
| Trypsin | - |
Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.011 | - |
D-mannitol[side 1] | at pH 5.5 and 30°C | Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | integral membrane protein | Escherichia coli | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| [protein]-L-histidine + D-mannitol 1-phosphate[side 2] | Escherichia coli | - |
[protein]-Npi-phospho-L-histidine + D-mannitol[side 1] | - |
r | |
| [protein]-Npi-phospho-L-histidine + D-mannitol[side 1] | Escherichia coli | - |
[protein]-L-histidine + D-mannitol 1-phosphate[side 2] | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P00550 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-cellulose column chromatography and hexyl agarose column chromatography | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the purified enzyme does not catalyze hydrolysis of D-mannitol 1-phosphate | Escherichia coli | ? | - |
? | |
| [protein]-L-histidine + D-mannitol 1-phosphate[side 2] | - |
Escherichia coli | [protein]-Npi-phospho-L-histidine + D-mannitol[side 1] | - |
r | |
| [protein]-Npi-phospho-L-histidine + D-mannitol[side 1] | - |
Escherichia coli | [protein]-L-histidine + D-mannitol 1-phosphate[side 2] | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| enzyme IImtl | - |
Escherichia coli |
| mannitol-specific enzyme II | - |
Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Escherichia coli |
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