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Literature summary for 2.7.1.19 extracted from

  • Michels, A.K.; Wedel, N.; Kroth, P.G.
    Diatom plastids possess a phosphoribulokinase with an altered regulation and no oxidative pentose phosphate pathway (2005), Plant Physiol., 137, 911-920.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.19

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol reduced form Trieres chinensis
thioredoxin reduced form, regulates activity via reversible reduction Trieres chinensis
thioredoxin reduced form, regulates activity via reversible reduction, probably not relevant in vivo Spinacia oleracea

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Trieres chinensis

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoic acid) DTNB, Ellman’s reagent, almost complete inhibition at 0.0045 mM Trieres chinensis
Cu2+
-
 Trieres chinensis
dithiothreitol oxidized form Trieres chinensis
glutathione oxidized form Trieres chinensis
additional information inhibited by addition of strong oxidants, reversible by addition of reducing agents Trieres chinensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.084
-
 ATP pH 8 Trieres chinensis
0.118
-
 D-ribulose 5-phosphate pH 8 Trieres chinensis

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Trieres chinensis 9507
-
chloroplast
-
 Spinacia oleracea 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 2 * 44000, SDS-PAGE, native mass by gel filtration Trieres chinensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-ribulose 5-phosphate Trieres chinensis part of the Calvin cycle (reductive pentose phosphate pathway), involved in CO2 fixation in photosynthetic organisms ADP + D-ribulose 1,5-bisphosphate
-
 ?
ATP + D-ribulose 5-phosphate Spinacia oleracea part of the Calvin cycle (reductive pentose phosphate pathway), involved in CO2 fixation in photosynthetic organisms ADP + D-ribulose 1,5-bisphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Spinacia oleracea P09559
-
-
Trieres chinensis Q95033
-
-

Purification (Commentary)

Purification (Comment) Organism
from chloroplasts Spinacia oleracea
recombinant enzyme Trieres chinensis

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
300
-
 purified enzyme, pH 8 Trieres chinensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-ribulose 5-phosphate
-
 Trieres chinensis ADP + D-ribulose 1,5-bisphosphate
-
 ?
ATP + D-ribulose 5-phosphate
-
 Spinacia oleracea ADP + D-ribulose 1,5-bisphosphate
-
 ?
ATP + D-ribulose 5-phosphate part of the Calvin cycle (reductive pentose phosphate pathway), involved in CO2 fixation in photosynthetic organisms Trieres chinensis ADP + D-ribulose 1,5-bisphosphate
-
 ?
ATP + D-ribulose 5-phosphate part of the Calvin cycle (reductive pentose phosphate pathway), involved in CO2 fixation in photosynthetic organisms Spinacia oleracea ADP + D-ribulose 1,5-bisphosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 44000, SDS-PAGE, native mass by gel filtration Trieres chinensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10
-
 stable for at least 2 h Trieres chinensis
25
-
 unstable when assay is performed at 25°C Trieres chinensis