Cloned (Comment) | Organism |
---|---|
wild-type and mutants | Cereibacter sphaeroides |
Protein Variants | Comment | Organism |
---|---|---|
K165C | reduced catalytic activity | Cereibacter sphaeroides |
K165M | reduced catalytic activity, insensitive to inactivation by pyridoxal 5'-phosphate | Cereibacter sphaeroides |
K53M | sensitive to inactivation by pyridoxal 5'-phosphate | Cereibacter sphaeroides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-phospho-D-gluconate | competitive with respect to D-ribulose 5-phosphate | Cereibacter sphaeroides | |
pyridoxal 5'-phosphate | inactivation is protected by ATP in combination with NADH | Cereibacter sphaeroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.096 | - |
D-ribulose 5-phosphate | wild-type enzyme, pH 8, 30°C | Cereibacter sphaeroides | |
10.3 | - |
D-ribulose 5-phosphate | K165M mutant, pH 8, 30°C | Cereibacter sphaeroides | |
11 | - |
D-ribulose 5-phosphate | K165C mutant, pH 8, 30°C | Cereibacter sphaeroides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cereibacter sphaeroides | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutant enzymes | Cereibacter sphaeroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-ribulose 5-phosphate + ATP | - |
Cereibacter sphaeroides | D-ribulose 1,5-diphosphate + ADP | - |
? | |
D-ribulose 5-phosphate + trinitrophenyl-ATP | spectroscopically active ATP-analog | Cereibacter sphaeroides | D-ribulose 1,5-diphosphate + trinitrophenyl-ADP | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Cereibacter sphaeroides |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.222 | - |
6-phospho-D-gluconate | wild-type enzyme, pH 8, 30°C | Cereibacter sphaeroides | |
0.415 | - |
6-phospho-D-gluconate | R168Q mutant, pH 8, 30°C | Cereibacter sphaeroides | |
1.15 | - |
6-phospho-D-gluconate | R173Q mutant, pH 8, 30°C | Cereibacter sphaeroides | |
2.81 | - |
6-phospho-D-gluconate | H45N mutant, pH 8, 30°C | Cereibacter sphaeroides | |
11 | - |
6-phospho-D-gluconate | K165M mutant, pH 8, 30°C | Cereibacter sphaeroides | |
19.8 | - |
6-phospho-D-gluconate | R49Q mutant, pH 8, 30°C | Cereibacter sphaeroides |