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Literature summary for 2.7.1.19 extracted from

  • Saharan, M.M.R.; Singh, R.
    Purification and characterization of phosphoribulokinase from immature pods of Brassica (1993), J. Plant Biochem. Biotechnol., 2, 121-124.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
D-glucose 1-phosphate activation Brassica sp.
D-glucose 6-phosphate activation Brassica sp.

Inhibitors

Inhibitors Comment Organism Structure
6-phospho-D-gluconate
-
Brassica sp.
AMP
-
Brassica sp.
D-Ribulose 1,5-diphosphate
-
Brassica sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
ATP
-
Brassica sp.
0.29 0.33 D-ribulose 5-phosphate
-
Brassica sp.

Metals/Ions

Metals/Ions Comment Organism Structure
K+ required Brassica sp.
Mg2+ required Brassica sp.
phosphate activation Brassica sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
1 * 48000 + 1 * 47000 + 1 * 41000 + 1 * 33000, SDS-PAGE Brassica sp.
41000
-
1 * 48000 + 1 * 47000 + 1 * 41000 + 1 * 33000, SDS-PAGE Brassica sp.
47000
-
1 * 48000 + 1 * 47000 + 1 * 41000 + 1 * 33000, SDS-PAGE Brassica sp.
48000
-
1 * 48000 + 1 * 47000 + 1 * 41000 + 1 * 33000, SDS-PAGE Brassica sp.
180000
-
gel filtration Brassica sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-ribulose 5-phosphate + ATP Brassica sp.
-
D-ribulose 1,5-diphosphate + ADP
-
?

Organism

Organism UniProt Comment Textmining
Brassica sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Brassica sp.

Source Tissue

Source Tissue Comment Organism Textmining
pod
-
Brassica sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribulose 5-phosphate + ATP
-
Brassica sp. D-ribulose 1,5-diphosphate + ADP
-
?

Subunits

Subunits Comment Organism
tetramer 1 * 48000 + 1 * 47000 + 1 * 41000 + 1 * 33000, SDS-PAGE Brassica sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.4
-
Brassica sp.