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Literature summary for 2.7.1.19 extracted from
- Purification and regulatory properties of phosphoribulokinase from Hydrogenomonas eutropha H 16 (1974), Biochem. J., 139, 481-489.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| NADH | 1 mM | Cupriavidus necator |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AMP | 50% inhibition at 0.08 mM | Cupriavidus necator | |
| phosphoenolpyruvate | - |
Cupriavidus necator | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Cupriavidus necator |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Cupriavidus necator | |
| Mg2+ | Km: ca. 0.5 mM | Cupriavidus necator | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 230000 | - |
gel filtration | Cupriavidus necator |
| 237000 | - |
- |
Cupriavidus necator |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-ribulose 5-phosphate + ATP | Cupriavidus necator | - |
D-ribulose 1,5-diphosphate + ADP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cupriavidus necator | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 286fold | Cupriavidus necator |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 88.9 | - |
- |
Cupriavidus necator |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 0.02 M triethanolamine-HCl buffer, pH 7.6, 0.5 mM EDTA, 0.5 mM dithiothreitol, 4 months | Cupriavidus necator |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-ribulose 5-phosphate + ATP | - |
Cupriavidus necator | D-ribulose 1,5-diphosphate + ADP | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Cupriavidus necator |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | - |
50% of maximal activity | Cupriavidus necator |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | activation | Cupriavidus necator | |
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