Any feedback?
Literature summary for 2.7.1.19 extracted from
- Purification and molecular and catalytic properties of phosphoribulokinase from the cyanobacterium Chlorogloeopsis fritschii (1984), J. Gen. Microbiol., 130, 999-1006.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | activation in semi-purified extracts | Chlorogloeopsis fritschii |  |
| additional information | no activation by dithiothreitol | Chlorogloeopsis fritschii | |
| additional information | not activated by NAD+, NADP+, NADPH, reduced glutathione | Chlorogloeopsis fritschii | |
| reduced glutathione | activation in semi-purified extracts | Chlorogloeopsis fritschii | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | competitive, 1 mM | Chlorogloeopsis fritschii | |
| AMP | competitive, 1 mM | Chlorogloeopsis fritschii | |
| Ca2+ | 200 mM | Chlorogloeopsis fritschii | |
| Co2+ | 200 mM | Chlorogloeopsis fritschii | |
| L-aspartate | competitive, 1 mM | Chlorogloeopsis fritschii | |
| L-malate | competitive, 1 mM | Chlorogloeopsis fritschii | |
| Mn2+ | 200 mM | Chlorogloeopsis fritschii | |
| phosphoenolpyruvate | competitive, 1 mM | Chlorogloeopsis fritschii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | can partially replace Mg2+ | Chlorogloeopsis fritschii | |
| Ca2+ | 5 mM | Chlorogloeopsis fritschii | |
| Mg2+ | required | Chlorogloeopsis fritschii | |
| Mn2+ | can partially replace Mg2+ | Chlorogloeopsis fritschii | |
| Mn2+ | 5 mM | Chlorogloeopsis fritschii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
6 * 40000, SDS-PAGE | Chlorogloeopsis fritschii |
| 230000 | - |
gel filtration | Chlorogloeopsis fritschii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-ribulose 5-phosphate + ATP | Chlorogloeopsis fritschii | - |
D-ribulose 1,5-diphosphate + ADP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chlorogloeopsis fritschii | - |
cyanobacterium | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Chlorogloeopsis fritschii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14.24 | - |
- |
Chlorogloeopsis fritschii |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, no loss of activity for 4 weeks | Chlorogloeopsis fritschii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-ribulose 5-phosphate + ATP | - |
Chlorogloeopsis fritschii | D-ribulose 1,5-diphosphate + ADP | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 40000, SDS-PAGE | Chlorogloeopsis fritschii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.6 | - |
- |
Chlorogloeopsis fritschii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | no activation | Chlorogloeopsis fritschii | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
