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Literature summary for 2.7.1.19 extracted from

  • Rippel, S.; Bowien, B.
    Phosphoribulokinase from Rhodospseudomonas acidophila (1984), Arch. Microbiol., 139, 207-212.
No PubMed abstract available
Search for more literature for 2.7.1.19

Activating Compound

Activating Compound Comment Organism Structure
NADH strong activator Rhodoblastus acidophilus

Inhibitors

Inhibitors Comment Organism Structure
phosphoenolpyruvate 50% inhibition at 0.32 mM Rhodoblastus acidophilus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Rhodoblastus acidophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
248000
-
 sucrose density gradient centrifugation Rhodoblastus acidophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-ribulose 5-phosphate + ATP Rhodoblastus acidophilus
-
 D-ribulose 1,5-diphosphate + ADP
-
 ?
D-ribulose 5-phosphate + ATP Rhodoblastus acidophilus 1150
-
 D-ribulose 1,5-diphosphate + ADP
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodoblastus acidophilus
-
 strain 01150
-
Rhodoblastus acidophilus 1150
-
 strain 01150
-

Purification (Commentary)

Purification (Comment) Organism
homogeneity Rhodoblastus acidophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
111.2
-
-
 Rhodoblastus acidophilus

Storage Stability

Storage Stability Organism
0-4°C, 100 mM imidazole-HCl buffer, pH 7.0, 10 mM MgCl2, 1 mM EDTA, 1 mM dithioerythritol, 3 months Rhodoblastus acidophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribulose 5-phosphate + ATP
-
 Rhodoblastus acidophilus D-ribulose 1,5-diphosphate + ADP
-
 ?
D-ribulose 5-phosphate + ATP ATP can be substituted by CTP with reduced activity Rhodoblastus acidophilus D-ribulose 1,5-diphosphate + ADP
-
 ?
D-ribulose 5-phosphate + ATP ATP can be substituted by UTP with reduced activity Rhodoblastus acidophilus D-ribulose 1,5-diphosphate + ADP
-
 ?
D-ribulose 5-phosphate + ATP ATP can be substituted by GTP with reduced activity Rhodoblastus acidophilus D-ribulose 1,5-diphosphate + ADP
-
 ?
D-ribulose 5-phosphate + ATP ATP can be substituted by TTP with reduced activity Rhodoblastus acidophilus D-ribulose 1,5-diphosphate + ADP
-
 ?
D-ribulose 5-phosphate + ATP
-
 Rhodoblastus acidophilus 1150 D-ribulose 1,5-diphosphate + ADP
-
 ?
D-ribulose 5-phosphate + ATP ATP can be substituted by CTP with reduced activity Rhodoblastus acidophilus 1150 D-ribulose 1,5-diphosphate + ADP
-
 ?
D-ribulose 5-phosphate + ATP ATP can be substituted by UTP with reduced activity Rhodoblastus acidophilus 1150 D-ribulose 1,5-diphosphate + ADP
-
 ?
D-ribulose 5-phosphate + ATP ATP can be substituted by GTP with reduced activity Rhodoblastus acidophilus 1150 D-ribulose 1,5-diphosphate + ADP
-
 ?
D-ribulose 5-phosphate + ATP ATP can be substituted by TTP with reduced activity Rhodoblastus acidophilus 1150 D-ribulose 1,5-diphosphate + ADP
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 presence of NADH Rhodoblastus acidophilus
8.4
-
 absence of NADH Rhodoblastus acidophilus

Cofactor

Cofactor Comment Organism Structure
NADH activation Rhodoblastus acidophilus
NADH pH-dependent Rhodoblastus acidophilus