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Literature summary for 2.7.1.19 extracted from

  • Serra, J.L.; Llama, M.J.; Rowell, P.; Stewart, W.D.P.
    Purification and characterization of phosphoribulokinase from the N2-fixing cyanobacterium Anabaena cylindrica (1989), Plant Sci., 59, 1-9.
No PubMed abstract available
Search for more literature for 2.7.1.19

Activating Compound

Activating Compound Comment Organism Structure
thioredoxin activation by ditiothreitol-reduced thioredoxin, 3.3fold Anabaena cylindrica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.29
-
 D-ribulose 5-phosphate pH 7.5, 30°C Anabaena cylindrica
1.42
-
 ATP pH 7.5, 30°C Anabaena cylindrica

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ no activity Anabaena cylindrica
Mg2+ required Anabaena cylindrica
Mn2+ no activity Anabaena cylindrica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 1 * 43000 + 1 * 26000, SDS-PAGE in presence of 2-mercaptoethanol Anabaena cylindrica
43000
-
 1 * 43000 + 1 * 26000, SDS-PAGE in presence of 2-mercaptoethanol Anabaena cylindrica
72000
-
 gel filtration Anabaena cylindrica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-ribulose 5-phosphate + ATP Anabaena cylindrica
-
 D-ribulose 1,5-diphosphate + ADP
-
 ?

Organism

Organism UniProt Comment Textmining
Anabaena cylindrica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Anabaena cylindrica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.95
-
-
 Anabaena cylindrica
15.17
-
 after activation by reduced thioredoxin Anabaena cylindrica

Storage Stability

Storage Stability Organism
-20°C, 50 mM Tris-HCl buffer, pH 8.0, 1 mM 2-mercaptoethanol Anabaena cylindrica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-ribulose 5-phosphate + ATP
-
 Anabaena cylindrica D-ribulose 1,5-diphosphate + ADP
-
 ?
D-ribulose 5-phosphate + ATP ATP can be substituted by CTP with reduced activity Anabaena cylindrica D-ribulose 1,5-diphosphate + ADP
-
 ?
D-ribulose 5-phosphate + ATP ATP can be substituted by UTP with reduced activity Anabaena cylindrica D-ribulose 1,5-diphosphate + ADP
-
 ?
D-ribulose 5-phosphate + ATP ATP can be substituted by GTP with reduced activity Anabaena cylindrica D-ribulose 1,5-diphosphate + ADP
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 43000 + 1 * 26000, SDS-PAGE in presence of 2-mercaptoethanol Anabaena cylindrica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6
-
-
 Anabaena cylindrica