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Literature summary for 2.7.1.185 extracted from

  • Rossoni, L.; Hall, S.J.; Eastham, G.; Licence, P.; Stephens, G.
    The putative mevalonate diphosphate decarboxylase from Picrophilus torridus is in reality a mevalonate-3-kinase with high potential for bioproduction of isobutene (2015), Appl. Environ. Microbiol., 81, 2625-2634.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis isobutene production rates are 507 pmol/min*g cells using Escherichia coli cells expressing the enzyme and 2880 pmol/min*mg protein with the purified histidine-tagged enzyme. Isobutene is a small, highly reactive molecule, used extensively as a platform chemical to manufacture a wide variety of products including fuel additives, rubbers, and speciality chemicals Picrophilus torridus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Picrophilus torridus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.023
-
ATP pH 7.5, 30┬░C Picrophilus torridus
0.131
-
(R)-mevalonate pH 7.5, 30┬░C Picrophilus torridus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (R)-mevalonate Picrophilus torridus key enzyme of the Thermoplasma acidophilum-type mevalonate pathway ADP + (R)-3-phosphomevalonate
-
?
ATP + (R)-mevalonate Picrophilus torridus DSM 9790 key enzyme of the Thermoplasma acidophilum-type mevalonate pathway ADP + (R)-3-phosphomevalonate
-
?

Organism

Organism UniProt Comment Textmining
Picrophilus torridus Q6KZB1
-
-
Picrophilus torridus DSM 9790 Q6KZB1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Picrophilus torridus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-mevalonate
-
Picrophilus torridus ADP + (R)-3-phosphomevalonate
-
?
ATP + (R)-mevalonate key enzyme of the Thermoplasma acidophilum-type mevalonate pathway Picrophilus torridus ADP + (R)-3-phosphomevalonate
-
?
ATP + (R)-mevalonate
-
Picrophilus torridus DSM 9790 ADP + (R)-3-phosphomevalonate
-
?
ATP + (R)-mevalonate key enzyme of the Thermoplasma acidophilum-type mevalonate pathway Picrophilus torridus DSM 9790 ADP + (R)-3-phosphomevalonate
-
?
ATP + 3-hydroxyisovalerate the potential of the enzyme in isobutene formation is due to the conversion of 3-hydroxyisovalerate to an unstable 3-phosphate intermediate that undergoes consequent spontaneous decarboxylation to form isobutene Picrophilus torridus ADP + phosphate + isobutene + CO2
-
?
ATP + 3-hydroxyisovalerate the potential of the enzyme in isobutene formation is due to the conversion of 3-hydroxyisovalerate to an unstable 3-phosphate intermediate that undergoes consequent spontaneous decarboxylation to form isobutene Picrophilus torridus DSM 9790 ADP + phosphate + isobutene + CO2
-
?

Synonyms

Synonyms Comment Organism
M3K
-
Picrophilus torridus
PTO1356 locus name Picrophilus torridus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.9
-
(R)-mevalonate pH 7.5, 30┬░C Picrophilus torridus

General Information

General Information Comment Organism
metabolism key enzyme of the Thermoplasma acidophilum-type mevalonate pathway Picrophilus torridus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
15
-
(R)-mevalonate pH 7.5, 30┬░C Picrophilus torridus