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Literature summary for 2.7.1.178 extracted from
- Characterization of Sulfolobus solfataricus 2-keto-3-deoxy-D-gluconate kinase in the modified Entner-Doudoroff pathway (2006), Biosci. Biotechnol. Biochem., 70, 1308-1316.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Saccharolobus solfataricus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.14 | - |
2-dehydro-3-deoxy-D-gluconate | pH 7.2, 60°C | Saccharolobus solfataricus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34874 | - |
2 * 34874, calculated from sequence | Saccharolobus solfataricus |
| 43000 | - |
2 * 43000, SDS-PAGE | Saccharolobus solfataricus |
| 72000 | - |
gel filtration | Saccharolobus solfataricus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2-dehydro-3-deoxy-D-gluconate | Saccharolobus solfataricus | the enzyme from the archaeon Sulfolobus solfataricus is involved in glucose catabolism via the branched variant of the EntnerDoudoroff pathway | ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate | - |
? | |
| ATP + 2-dehydro-3-deoxy-D-gluconate | Saccharolobus solfataricus P2 | the enzyme from the archaeon Sulfolobus solfataricus is involved in glucose catabolism via the branched variant of the EntnerDoudoroff pathway | ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | Q97U29 | - |
- |
| Saccharolobus solfataricus P2 | Q97U29 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2-dehydro-3-deoxy-D-gluconate | - |
Saccharolobus solfataricus | ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate | - |
? | |
| ATP + 2-dehydro-3-deoxy-D-gluconate | the enzyme from the archaeon Sulfolobus solfataricus is involved in glucose catabolism via the branched variant of the EntnerDoudoroff pathway | Saccharolobus solfataricus | ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate | - |
? | |
| ATP + 2-dehydro-3-deoxy-D-gluconate | - |
Saccharolobus solfataricus P2 | ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate | - |
? | |
| ATP + 2-dehydro-3-deoxy-D-gluconate | the enzyme from the archaeon Sulfolobus solfataricus is involved in glucose catabolism via the branched variant of the EntnerDoudoroff pathway | Saccharolobus solfataricus P2 | ADP + 6-phospho-2-dehydro-3-deoxy-D-gluconate | - |
? | |
| additional information | no activity with 2-dehydro-D-gluconate or 5-dehydro-D-gluconate | Saccharolobus solfataricus | ? | - |
? | |
| additional information | no activity with 2-dehydro-D-gluconate or 5-dehydro-D-gluconate | Saccharolobus solfataricus P2 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 43000, SDS-PAGE | Saccharolobus solfataricus |
| homodimer | 2 * 34874, calculated from sequence | Saccharolobus solfataricus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| KDGK | - |
Saccharolobus solfataricus |
| SSO3195 | - |
Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
assay at | Saccharolobus solfataricus |
| 70 | 80 | - |
Saccharolobus solfataricus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
deactivated above | Saccharolobus solfataricus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 60.8 | - |
2-dehydro-3-deoxy-D-gluconate | pH 7.2, 60°C | Saccharolobus solfataricus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
Saccharolobus solfataricus |
| 7.2 | - |
assay at | Saccharolobus solfataricus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Saccharolobus solfataricus | calculated from sequence | - |
6.1 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 434 | - |
2-dehydro-3-deoxy-D-gluconate | pH 7.2, 60°C | Saccharolobus solfataricus | |
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