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Literature summary for 2.7.1.177 extracted from
- Kinetic and functional analysis of L-threonine kinase, the PduX enzyme of Salmonella enterica (2009), J. Biol. Chem., 284, 20240-20248.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D103N | similar growth rate compared to wild-type | Salmonella enterica |
| D135A | no activity, decrease in helical structure | Salmonella enterica |
| D135N | similar growth rate compared to wild-type | Salmonella enterica |
| E132A | similar growth rate compared to wild-type | Salmonella enterica |
| E132Q | similar growth rate compared to wild-type | Salmonella enterica |
| E24A | no activity, decrease in helical structure | Salmonella enterica |
| E24Q | similar growth rate compared to wild-type | Salmonella enterica |
| S100A | no activity | Salmonella enterica |
| S253A | low growth rate, reduced affinity for L-threonine | Salmonella enterica |
| S255A | low growth rate, reduced affinity for L-threonine | Salmonella enterica |
| S99A | similar growth rate compared to wild-type | Salmonella enterica |
| T101A | similar growth rate compared to wild-type | Salmonella enterica |
| T134A | similar growth rate compared to wild-type | Salmonella enterica |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0547 | - |
ATP | wild-type, pH 7.4, temperature not specified in the publication | Salmonella enterica |  |
| 0.068 | - |
ATP | mutant D135N, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.074 | - |
ATP | mutant S255A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.076 | - |
ATP | mutant S253A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.087 | - |
ATP | mutant T134A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.098 | - |
ATP | mutant T101A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.117 | - |
ATP | mutant E24Q, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.141 | - |
ATP | mutant D103N, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.142 | - |
L-threonine | mutant T134A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.144 | - |
L-threonine | mutant E132Q, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.146 | - |
L-threonine | wild-type, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.163 | - |
ATP | mutant D103A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.176 | - |
L-threonine | mutant S99A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.184 | - |
L-threonine | mutant D103N, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.198 | - |
L-threonine | mutant E132A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.22 | - |
L-threonine | mutant T101A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.258 | - |
L-threonine | mutant D103A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.273 | - |
L-threonine | mutant D135N, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.464 | - |
L-threonine | mutant E24Q, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 1.264 | - |
ATP | mutant E132Q, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 1.374 | - |
ATP | mutant S99A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 1.979 | - |
ATP | mutant E132A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 3.708 | - |
L-threonine | mutant S255A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 4.698 | - |
L-threonine | mutant S253A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
x * 35000, SDS-PAGE | Salmonella enterica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-threonine | Salmonella enterica | - |
ADP + O-phospho-L-threonine | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica | Q9XDM4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nickel-nitrilotriacetate column | Salmonella enterica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-threonine | - |
Salmonella enterica | ADP + O-phospho-L-threonine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 35000, SDS-PAGE | Salmonella enterica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PduX | - |
Salmonella enterica |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.016 | - |
L-threonine | mutant D135N, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.0173 | - |
ATP | mutant D135N, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.0187 | - |
ATP | mutant E24Q, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.0188 | - |
L-threonine | mutant E24Q, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.0193 | - |
L-threonine | mutant E132A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.0205 | - |
ATP | mutant E132A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.0227 | - |
ATP | mutant S255A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.0238 | - |
L-threonine | mutant S255A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.0258 | - |
L-threonine | mutant E132Q, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.026 | - |
ATP | mutant S253A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.0272 | - |
ATP | mutant E132Q, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.0287 | - |
L-threonine | mutant S253A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.0313 | - |
ATP | mutant D103A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.0322 | - |
L-threonine | mutant D103A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.0325 | - |
L-threonine | mutant S99A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.0338 | - |
ATP | mutant D103N, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.0338 | - |
ATP | mutant S99A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.0342 | - |
ATP | mutant T134A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.0353 | - |
L-threonine | mutant T134A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.0355 | - |
ATP | mutant T101A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.0358 | - |
L-threonine | mutant D103N, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.037 | - |
L-threonine | mutant T101A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.0427 | - |
L-threonine | wild-type, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.408 | - |
ATP | wild-type, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | involved in the biosynthesis of adenosylcobalamin | Salmonella enterica |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000061 | - |
L-threonine | mutant S253A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.0000643 | - |
L-threonine | mutant S255A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.000104 | - |
ATP | mutant E132A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.00022 | - |
ATP | mutant E132Q, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.000247 | - |
ATP | mutant S99A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.000407 | - |
L-threonine | mutant E24Q, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.000587 | - |
L-threonine | mutant D135N, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.00098 | - |
L-threonine | mutant E132A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.00125 | - |
L-threonine | mutant D103A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.00159 | - |
ATP | mutant E24Q, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.00168 | - |
L-threonine | mutant T101A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.0018 | - |
L-threonine | mutant E132Q, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.00185 | - |
L-threonine | mutant S99A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.00193 | - |
ATP | mutant D103A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.00195 | - |
L-threonine | mutant D103N, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.0024 | - |
ATP | mutant D103N, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.00248 | - |
L-threonine | mutant T134A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.00257 | - |
ATP | mutant D135N, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.00292 | - |
L-threonine | wild-type, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.00308 | - |
ATP | mutant S255A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.00342 | - |
ATP | mutant S253A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.00363 | - |
ATP | mutant T101A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.00393 | - |
ATP | mutant T134A, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
| 0.00755 | - |
ATP | wild-type, pH 7.4, temperature not specified in the publication | Salmonella enterica | |
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