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Literature summary for 2.7.1.175 extracted from
- Biochemical characterization of the maltokinase from Mycobacterium bovis BCG (2010), BMC Biochem., 11, 21.
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | Mak may be a potential drug target in Mycobacterium tuberculosis | Mycobacterium tuberculosis variant bovis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene mak, DNA and amino acid sequence determination and analysis, high level overexpression of His-tagged Mak in Escherichia coli | Mycobacterium tuberculosis variant bovis |
General Stability
| General Stability | Organism |
|---|---|
| addition of 50 mM NaCl markedly enhances Mak stability | Mycobacterium tuberculosis variant bovis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| glycerol | strongly inhibits Mak activity, even at very low concentrations of 1% | Mycobacterium tuberculosis variant bovis |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | recombinant Mak exhibits Michaelis-Menten kinetics at 37°C with ATP, GTP and UTP up to 5 mM | Mycobacterium tuberculosis variant bovis | |
| 0.74 | - |
ATP | pH 8.0, 37°C, recombinant His-tagged Mak | Mycobacterium tuberculosis variant bovis | |
| 2.52 | - |
maltose | pH 8.0, 37°C, recombinant His-tagged Mak | Mycobacterium tuberculosis variant bovis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activates, 41% activity compared to Mg2+ | Mycobacterium tuberculosis variant bovis | |
| Mg2+ | best activating cation | Mycobacterium tuberculosis variant bovis | |
| Mn2+ | activates, 14% activity compared to Mg2+ | Mycobacterium tuberculosis variant bovis | |
| additional information | divalent cations are required for activity and Mg2+ is the best activator | Mycobacterium tuberculosis variant bovis | |
| NaCl | addition of 50 mM NaCl markedly enhances Mak stability | Mycobacterium tuberculosis variant bovis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50700 | - |
1 * 50700, recombinant His-tagged Mak, SDS-PAGE | Mycobacterium tuberculosis variant bovis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + maltose | Mycobacterium tuberculosis variant bovis | - |
ADP + maltose 1-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis variant bovis | - |
gene mak | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli by anion exchange chromatography and gel filtration | Mycobacterium tuberculosis variant bovis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, purified recombinant His-tagged enzyme, 7 days, about 40% of activity remaining, at pH 7.5, most of the activity is lost after freeze/thawing at -20°C. 10 mM maltose only slightly improve the stability at 4°C to about 50% of maximal activity, but addition of 50 mM NaCl dramatically improve the stability of the enzyme with over 90% activity remaining after 1 week at 4°C | Mycobacterium tuberculosis variant bovis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + maltose | - |
Mycobacterium tuberculosis variant bovis | ADP + maltose 1-phosphate | - |
? | |
| ATP + maltose | - |
Mycobacterium tuberculosis variant bovis | ADP + maltose 1-phosphate | NMR spectroscopical product analysis | ? | |
| GTP + maltose | - |
Mycobacterium tuberculosis variant bovis | GDP + maltose 1-phosphate | NMR spectroscopical product analysis | ? | |
| UTP + maltose | - |
Mycobacterium tuberculosis variant bovis | UDP + maltose 1-phosphate | NMR spectroscopical product analysis | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 50700, recombinant His-tagged Mak, SDS-PAGE | Mycobacterium tuberculosis variant bovis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Mak | - |
Mycobacterium tuberculosis variant bovis |
| maltose-1-phosphate synthetase | - |
Mycobacterium tuberculosis variant bovis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Mycobacterium tuberculosis variant bovis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 65 | activity range | Mycobacterium tuberculosis variant bovis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | at 37°C | Mycobacterium tuberculosis variant bovis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 11 | activity range | Mycobacterium tuberculosis variant bovis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | dependent on | Mycobacterium tuberculosis variant bovis | |
| GTP | can substitute for ATP | Mycobacterium tuberculosis variant bovis | |
| UTP | can substitute for ATP | Mycobacterium tuberculosis variant bovis | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | genetic environment of the mak gene in different organisms. Organization of the region containing the mak gene, overview | Mycobacterium tuberculosis variant bovis |
| metabolism | involvement of maltose-1-phosphate in the regulation of sugar metabolism in Escherichia coli | Mycobacterium tuberculosis variant bovis |
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Release 2023.1 (January 2023)
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