Literature summary for 2.7.1.171 extracted from

Avemaria, F.; Carrera, P.; Lapolla, A.; Sartore, G.; Chilelli, N.C.; Paleari, R.; Ambrosi, A.; Ferrari, M.; Mosca, A.
Possible role of fructosamine 3-kinase genotyping for the management of diabetic patients (2015), Clin. Chem. Lab. Med., 53, 1315-1320.

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Cloned(Commentary)

Cloned (Comment)	Organism
gene FN3K, located on chromosome 17q25.3, genotyping	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	analysis of the FN3K gene on a Caucasian cohort of diabetic patients, molecular characterization of the FN3K gene by analyzing its promoter and of polymorphisms, c-385A/G (rs3859206) and the c-232A/T (rs2256339), associated with FN3K enzymatic activity in erythrocytes, as well as two variants c-421C/T and c-429delATCGGAG	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
deoxymorpholinofructose	a competitive inhibitor	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + [protein]-N6-D-fructosyl-L-lysine	Homo sapiens	-	ADP + [protein]-N6-(3-O-phospho-D-fructosyl)-L-lysine	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q9H479	gene FN3K	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
brain	-	Homo sapiens	-
erythrocyte	-	Homo sapiens	-
lens	-	Homo sapiens	-
additional information	FN3K is more active in tissues containing proteins with long (half-)lives, such as erythrocytes, lens and brain	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + [protein]-N6-D-fructosyl-L-lysine	-	Homo sapiens	ADP + [protein]-N6-(3-O-phospho-D-fructosyl)-L-lysine	-	?
additional information	the fructosamines bound to Lys139alpha, located near the C-terminus of the alpha subunits, and Lys16alpha, located on a loop of the alpha subunits, are good substrates. The N-terminal glycated valine is a poor substrate, consistent with free fructosevaline being a much poorer substrate than free fructoselysine	Homo sapiens	?	-	?

Synonyms

Synonyms	Comment	Organism
FN3K	-	Homo sapiens
fructosamine 3-kinase	-	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Homo sapiens

General Information

General Information	Comment	Organism
evolution	the FN3K gene may have arisen by an event of duplication of an ancestral gene, FN3K-related protein (FN3K-RP). The gene encoding FN3K-RP is located next to the one encoding FN3K, and share a 65% sequence homology with FN3K and an identical genome organization. Both FN3K and FN3K-RP phosphorylate psicosamines and ribulosamines, but only the former act on fructosamines	Homo sapiens
metabolism	starvation and diabetes do not change the level of expression of FN3K in different tissues, and no regulation of FN3K expression is observed in human fibroblasts treated with condition mimicking the diabetic state	Homo sapiens
physiological function	fructosamine 3-kinase (FN3K) is involved in protein deglycation FN3K phosphorylates fructosamines on the third carbon of their sugar moiety, making them unstable and causing them to detach from proteins, suggesting a protective role of this enzyme. FN3K is able to break down the second intermediate of the non-enzymatic glycation cascade by phosphorylating fructoselysine to a fructoselysine-3-phosphate. The variability in FN3K activity is associated with some polymorphisms in the FN3K gene, FN3K involvement in diabetes, overview. FN3K might act in concert with other molecular mechanisms and may impact on gene expression and activity of other enzymes involved in deglycation process	Homo sapiens

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Release 2023.1 (January 2023)