Cloned (Comment) | Organism |
---|---|
human FN3K-RP is transfected in human embryonic kidney cells and expressed | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-deoxy-1-morpholin-4-yl-D-fructose | - |
Homo sapiens | |
1-deoxy-1-morpholin-4-yl-D-psicose | - |
Homo sapiens | |
1-deoxy-1-morpholin-4-yl-D-ribulose | - |
Homo sapiens | |
N6-D-psicosyl-L-lysine | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0026 | - |
1-deoxy-1-morpholin-4-yl-D-ribulose | pH 7.8, 30°C | Homo sapiens | |
0.01 | - |
1-deoxy-1-morpholin-4-yl-D-fructose | pH 7.8, 30°C | Homo sapiens | |
0.14 | - |
N6-D-psicosyl-L-lysine | pH 7.8, 30°C | Homo sapiens | |
0.16 | - |
1-deoxy-1-morpholin-4-yl-D-psicose | pH 7.8, 30°C | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [protein]-N5-D-ribulosyl-L-lysine | Homo sapiens | proteins glycated with allose, ketosamine-3-kinase 2 plays a role in freeing proteins from ribulosamines or psicosamines, which might arise in a several step process, from the reaction of amines with glucose and/or glycolytic intermediates. This role is shared by fructosamine-3-kinase (ketosamine-3-kinase 1), which has, in addition, the unique capacity to phosphorylate fructosamines | ADP + [protein]-N5-(O3-phosphono-D-fructosyl)-L-lysine | - |
? | |
ATP + [protein]-N6-D-psicosyl-L-lysine | Homo sapiens | ketosamine-3-kinase 2 plays a role in freeing proteins from ribulosamines or psicosamines, which might arise in a several step process, from the reaction of amines with glucose and/or glycolytic intermediates. This role is shared by fructosamine-3-kinase (ketosamine-3-kinase 1), which has, in addition, the unique capacity to phosphorylate fructosamines | ADP + [protein]-N6-(O3-phosphono-D-psicosyl)-L-lysine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9HA64 | - |
- |
Mus musculus | Q8K274 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, partially purified by chromatography on Blue Sepharose | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
bone marrow | highest levels of expression in bone marrow, brain, spleen and kidney | Mus musculus | - |
brain | highest levels of expression in bone marrow, brain, spleen and kidney | Mus musculus | - |
heart | - |
Mus musculus | - |
kidney | highest levels of expression in bone marrow, brain, spleen and kidney | Mus musculus | - |
liver | expressed at low levels | Mus musculus | - |
additional information | no expression is observed in the intestinal mucosa | Mus musculus | - |
skeletal muscle | expressed at low levels | Mus musculus | - |
spleen | highest levels of expression in bone marrow, brain, spleen and kidney | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-deoxy-1-morpholin-4-yl-D-fructose | - |
Homo sapiens | ADP + 1-deoxy-1-morpholin-4-yl-3-O-phosphono-D-fructose | - |
? | |
ATP + 1-deoxy-1-morpholin-4-yl-D-psicose | - |
Homo sapiens | ADP + 1-deoxy-1-morpholin-4-yl-3-O-phosphono-D-psicose | - |
? | |
ATP + 1-deoxy-1-morpholin-4-yl-D-ribulose | - |
Homo sapiens | ADP + 1-deoxy-1-morpholin-4-yl-3-O-phosphono-D-ribulose | - |
? | |
ATP + N6-D-psicosyl-L-lysine | - |
Homo sapiens | ADP + N6-(O3-phosphono-D-psicosyl)-L-lysine | - |
? | |
ATP + [protein]-N5-D-ribulosyl-L-lysine | proteins glycated with allose, ketosamine-3-kinase 2 plays a role in freeing proteins from ribulosamines or psicosamines, which might arise in a several step process, from the reaction of amines with glucose and/or glycolytic intermediates. This role is shared by fructosamine-3-kinase (ketosamine-3-kinase 1), which has, in addition, the unique capacity to phosphorylate fructosamines | Homo sapiens | ADP + [protein]-N5-(O3-phosphono-D-fructosyl)-L-lysine | - |
? | |
ATP + [protein]-N6-D-psicosyl-L-lysine | ketosamine-3-kinase 2 plays a role in freeing proteins from ribulosamines or psicosamines, which might arise in a several step process, from the reaction of amines with glucose and/or glycolytic intermediates. This role is shared by fructosamine-3-kinase (ketosamine-3-kinase 1), which has, in addition, the unique capacity to phosphorylate fructosamines | Homo sapiens | ADP + [protein]-N6-(O3-phosphono-D-psicosyl)-L-lysine | - |
? | |
additional information | FN3K-RP does not phosphorylate fructoselysine, 1-deoxy-1-morpholin-4-yl-D-fructose, or lysozyme glycated with glucose | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FN3K | - |
Homo sapiens |
FN3K | - |
Mus musculus |
ketosamine 3-kinase 1 | - |
Homo sapiens |
ketosamine 3-kinase 1 | - |
Mus musculus |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0036 | - |
pH 7.8, 30°C, phosphorylation of lysozyme glycated with allose | Homo sapiens | 1-deoxy-1-morpholin-4-yl-D-ribulose | |
0.067 | - |
pH 7.8, 30°C | Homo sapiens | 1-deoxy-1-morpholin-4-yl-D-fructose | |
0.69 | - |
pH 7.8, 30°C, phosphorylation of lysozyme glycated with allose | Homo sapiens | 1-deoxy-1-morpholin-4-yl-D-psicose | |
0.72 | - |
pH 7.8, 30°C, phosphorylation of lysozyme glycated with allose | Homo sapiens | N6-D-psicosyl-L-lysine |