Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.171 extracted from

  • Szwergold, B.S.; Howell, S.; Beisswenger, P.J.
    Human fructosamine-3-kinase: purification, sequencing, substrate specificity, and evidence of activity in vivo (2001), Diabetes, 50, 2139-2147.
    View publication on PubMed
Search for more literature for 2.7.1.171

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 [protein]-N6-D-fructosyl-L-lysine pH 8.0, 37°C Homo sapiens
0.5
-
 N5-D-fructosyl-L-ornithine pH 8.0, 37°C Homo sapiens
0.75
-
 N6-D-fructosyl-L-lysine pH 8.0, 37°C Homo sapiens
50
-
 D-fructose pH 8.0, 37°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 x * 35000, calculated from sequence Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [protein]-N6-D-fructosyl-L-lysine Homo sapiens nonenzymatic glycation is an important factor in the pathogenesis of diabetic complications. Key early intermediates in this process are fructosamines, such as protein-bound fructoselysines. The fructosamine most frequently encountered in nature is fructoselysine. Fructosamine-3-kinase is part of an ATP-dependent system for removing carbohydrates from nonenzymatically glycated proteins and protecting cells from the deleterious effects of nonenzymatic glycation ADP + [protein]-N6-(O3-phosphono-D-fructosyl)-L-lysine
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
ATP + [protein]-N6-D-fructosyl-L-lysine = ADP + [protein]-N6-(3-O-phospho-D-fructosyl)-L-lysine phosphorylation destablilizes the fructoselysine adduct and leads to its spontaneous decomposition Homo sapiens
a

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
 Homo sapiens
-
kidney the enzyme is present in all tissues, with the highest level of expression in the kidney Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.08
-
 pH 8.0, 37°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-fructose Vmax is 35% of the value for N6-D-fructosyl-L-lysine Homo sapiens ADP + O3-phosphono-D-fructose
-
 ?
ATP + N5-D-fructosyl-L-ornithine
-
 Homo sapiens ADP + N5-(O3-phosphono-D-fructosyl)-L-ornithine
-
 ?
ATP + N6-D-fructosyl-L-lysine
-
 Homo sapiens ADP + N6-(O3-phosphono-D-fructosyl)-L-lysine
-
 ?
ATP + [protein]-N6-D-fructosyl-L-lysine nonenzymatic glycation is an important factor in the pathogenesis of diabetic complications. Key early intermediates in this process are fructosamines, such as protein-bound fructoselysines. The fructosamine most frequently encountered in nature is fructoselysine. Fructosamine-3-kinase is part of an ATP-dependent system for removing carbohydrates from nonenzymatically glycated proteins and protecting cells from the deleterious effects of nonenzymatic glycation Homo sapiens ADP + [protein]-N6-(O3-phosphono-D-fructosyl)-L-lysine
-
 ?
ATP + [protein]-N6-D-fructosyl-L-lysine [histone]-N6-D-fructosyl-L-lysine, [hemoglobin]-N6-D-fructosyl-L-lysine. Similar experiments with other glycated proteins, including bovine serum albumin, and lysozyme indicate that fructoselysine residues on glycated proteins are readily phosphorylated by fructosamine 3-kinase, apparently irrespective of the protein. Phosphorylation destablilizes the fructoselysine adduct and leads to its spontaneous decomposition Homo sapiens ADP + [protein]-N6-(O3-phosphono-D-fructosyl)-L-lysine
-
 ?
additional information the kinase is specific for 1-deoxy-1-amino fructose adducts and does not catalyze phosphorylation of other monosaccharides and polyols, such as glucose, galactose, mannose, glucosamine, galactosamine, or myo-inositol Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 35000, calculated from sequence Homo sapiens

Synonyms

Synonyms Comment Organism
FN3K
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Homo sapiens