Literature summary for 2.7.1.17 extracted from

Martin del Campo, J.; Chun, Y.; Kim, J.; Patino, R.; Zhang, Y.
Discovery and characterization of a novel ATP/polyphosphate xylulokinase from a hyperthermophilic bacterium Thermotoga maritima (2013), J. Ind. Microbiol. Biotechnol., 40, 661-669 .

Search for more literature for 2.7.1.17

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 Star (DE3)	Thermotoga maritima

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.14	-	ATP	37°C, pH 7.5	Thermotoga maritima
0.22	-	D-xylulose	37°C, pH 7.5	Thermotoga maritima
0.7	-	ATP	60°C, pH 7.5	Thermotoga maritima
1.24	-	D-xylulose	60°C, pH 7.5	Thermotoga maritima
17	-	(Polyphosphate)n	37°C, pH 7.5	Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
60000	-	SDS-PAGE	Thermotoga maritima

Organism

Organism	UniProt	Comment	Textmining
Thermotoga maritima	Q9WXX1	-	-
Thermotoga maritima DSM 3109	Q9WXX1	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(polyphosphate)n + D-xylulose	activity with polyphosphate is 41 % of its specific activity on ATP	Thermotoga maritima	(polyphosphate)n-1 + D-xylulose 5-phosphate	-	?
(polyphosphate)n + D-xylulose	activity with polyphosphate is 41 % of its specific activity on ATP	Thermotoga maritima DSM 3109	(polyphosphate)n-1 + D-xylulose 5-phosphate	-	?
ATP + D-xylulose	-	Thermotoga maritima	ADP + D-xylulose 5-phosphate	-	?
ATP + D-xylulose	-	Thermotoga maritima DSM 3109	ADP + D-xylulose 5-phosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 60000, SDS-PAGE	Thermotoga maritima

Synonyms

Synonyms	Comment	Organism
ATP/polyphosphate xylulokinase	-	Thermotoga maritima
TM0116	-	Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
85	-	-	Thermotoga maritima

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
65	95	65°C: about 60% of maximal activity, 95°: about 60% of maximal activity	Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
60	-	degradation constants at 0.1 and 0.01 g/l xylulokinase at 60°C are 0.20/h and 0.35/h, respectively. The presence of 2 mM xylulose enhances the thermostability of 0.01 g/l xylulokinase, increasing degradation constants to 0.29/h	Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
8.77	-	(Polyphosphate)n	37°C, pH 7.5	Thermotoga maritima
24	-	ATP	37°C, pH 7.5	Thermotoga maritima
26	-	D-xylulose	37°C, pH 7.5	Thermotoga maritima
78	-	D-xylulose	60°C, pH 7.5	Thermotoga maritima
83	-	ATP	60°C, pH 7.5	Thermotoga maritima

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
62	-	D-xylulose	60°C, pH 7.5	Thermotoga maritima
116	-	D-xylulose	37°C, pH 7.5	Thermotoga maritima
119	-	ATP	60°C, pH 7.5	Thermotoga maritima
171	-	ATP	37°C, pH 7.5	Thermotoga maritima
516	-	(Polyphosphate)n	37°C, pH 7.5	Thermotoga maritima

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Release 2023.1 (January 2023)