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Literature summary for 2.7.1.17 extracted from

  • Komeda, H.; Yamasaki-Yashiki, S.; Hoshino, K.; Asano, Y.
    Identification and characterization of D-xylulokinase from the D-xylose-fermenting fungus, Mucor circinelloides (2014), FEMS Microbiol. Lett., 360, 51-61.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mucor circinelloides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.29
-
D-xylulose pH 7.4, 30°C Mucor circinelloides
0.51
-
ATP pH 7.4, 30°C Mucor circinelloides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57600
-
-
Mucor circinelloides
65100
-
gel filtration Mucor circinelloides

Organism

Organism UniProt Comment Textmining
Mucor circinelloides W8VYH1
-
-
Mucor circinelloides NBRC 4572 W8VYH1
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.8
-
pH 7.4, 30°C Mucor circinelloides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-xylulose
-
Mucor circinelloides ADP + D-xylulose 5-phosphate
-
?
ATP + D-xylulose
-
Mucor circinelloides NBRC 4572 ADP + D-xylulose 5-phosphate
-
?
ATP + L-ribulose 3.8% of the activity with D-xylulose Mucor circinelloides ADP + L-ribulose 5-phosphate
-
?
ATP + L-ribulose 3.8% of the activity with D-xylulose Mucor circinelloides NBRC 4572 ADP + L-ribulose 5-phosphate
-
?
ATP + L-xylulose 57.6% of the activity with D-xylulose Mucor circinelloides ADP + L-xylulose 5-phosphate
-
?
ATP + L-xylulose 57.6% of the activity with D-xylulose Mucor circinelloides NBRC 4572 ADP + L-xylulose 5-phosphate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 57600, calculated Mucor circinelloides

Synonyms

Synonyms Comment Organism
XYL3
-
Mucor circinelloides

Expression

Organism Comment Expression
Mucor circinelloides enzyme is induced by L-arabinose as well as D-xylose and the induction is repressed in the presence of D-glucose up