Literature summary for 2.7.1.17 extracted from

Lee, J.Y.; Cheong, D.E.; Kim, G.J.
A novel assay system for the measurement of transketolase activity using xylulokinase from Saccharomyces cerevisiae (2008), Biotechnol. Lett., 30, 899-904.

Search for more literature for 2.7.1.17

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli strain XL1-Blue (Stratagene)	Saccharomyces cerevisiae

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
68000	-	SDS-PAGE	Saccharomyces cerevisiae

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
harvested cells resuspended in 50 mM Tris/HCl pH 7.8, solution after ultrasonically disruption centrifuged at 10000 *g for 30 min at 4°C, supernatant passed through immobilized metal affinity chromatography (5 ml) using the His-tag introduced into the C-terminal region of XK, purified enzyme dialyzed against 50 mM Tris/Hcl (pH 7.8) containing 12.5% (w/v) glycerol	Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	20.6 +/-0.3 Unit/mg at 25°C, approx. 3 mM D-xylulose 5-phosphate could be produced over 10 min at 25°C from 5 mM xylulose with 0.004 mg purified xylulokinase. With 2.5 mM xylulose, about 80% of conversion occur.	Saccharomyces cerevisiae

Storage Stability

Storage Stability	Organism
-80°C, 50 mM Tris/HCl, pH 7.8, 12.5% (w/v) glycerol, 1 month	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + D-xylulose	-	Saccharomyces cerevisiae	ADP + D-xylulose 5-phosphate	-	r

Synonyms

Synonyms	Comment	Organism
D-xylulokinase	-	Saccharomyces cerevisiae

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)