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Literature summary for 2.7.1.17 extracted from
- Purification and properties of D-xylulokinase in liver (1958), J. Biol. Chem., 232, 737-748.
General Stability
| General Stability | Organism |
|---|---|
| repeated freeze-thawing inactivates, sulfhydryl compounds do not stabilize | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | product inhibition | Bos taurus |  |
| ATP | additional Mg2+ reverses | Bos taurus | |
| additional information | no inhibition by F- or iodoacetate | Bos taurus | |
| PCMB | cysteine reverses | Bos taurus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | no activation | Bos taurus | |
| Mg2+ | requirement, Mg2+/ATP ratio of at least 1 | Bos taurus | |
| Zn2+ | no activation | Bos taurus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-xylulose | Bos taurus | enzyme of ribitol catabolism, inducible by ribitol | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
calf | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.1 | - |
- |
Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -12°C, ammonium sulfate precipitate, over 1 month | Bos taurus |
| enzyme preparations are generally very unstable and lose their activity within a few days | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-xylulose | enzyme of ribitol catabolism, inducible by ribitol | Bos taurus | ? | - |
? | |
| ATP + D-xylulose | - |
Bos taurus | ADP + D-xylulose 5-phosphate | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | about half-maximal activity at pH 6 and 10 | Bos taurus |
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Release 2023.1 (January 2023)
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