Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DTT | enzyme is inactive in absence either EDTA or a thiol such as reduced monosodium glutathione or dithiothreitol | Klebsiella aerogenes | |
EDTA | enzyme is inactive in absence either EDTA or a thiol such as reduced monosodium glutathione or dithiothreitol | Klebsiella aerogenes | |
Monosodium glutathione | enzyme is inactive in absence either EDTA or a thiol such as reduced monosodium glutathione or dithiothreitol | Klebsiella aerogenes |
General Stability | Organism |
---|---|
D-xylulose, ATP, glycerol and EDTA stabilize | Klebsiella aerogenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | above 0.08 mM | Klebsiella aerogenes |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | MgCl2 required, optimal concentration: 0.05 mM | Klebsiella aerogenes | |
Mn2+ | 90% as effective as Mg2+ in activation | Klebsiella aerogenes | |
Ni2+ | activation, half as effective as Mg2+ | Klebsiella aerogenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-xylulose | Klebsiella aerogenes | enzyme of ribitol catabolism, inducible by ribitol | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella aerogenes | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Klebsiella aerogenes |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
95 | - |
- |
Klebsiella aerogenes |
Storage Stability | Organism |
---|---|
-20°C, about 30% of original activity retained after 4 years | Klebsiella aerogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-xylulose | enzyme of ribitol catabolism, inducible by ribitol | Klebsiella aerogenes | ? | - |
? | |
ATP + D-xylulose | specific for D-isomer | Klebsiella aerogenes | ADP + D-xylulose 5-phosphate | - |
r | |
ATP + xylitol | poor substrate | Klebsiella aerogenes | ADP + ? | - |
? | |
GTP + D-xylulose | phosphorylation at 25% the rate of ATP | Klebsiella aerogenes | GDP + D-xylulose 5-phosphate | - |
? | |
ITP + D-xylulose | phosphorylation at 25% the rate of ATP | Klebsiella aerogenes | IDP + D-xylulose 5-phosphate | - |
? | |
UTP + D-xylulose | phosphorylation at 14% the rate of ATP | Klebsiella aerogenes | UDP + D-xylulose 5-phosphate | - |
? |