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Literature summary for 2.7.1.17 extracted from

  • Simpson, F.J.
    D-Xylulokinase (1966), Methods Enzymol., 9, 454-458.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
DTT enzyme is inactive in absence either EDTA or a thiol such as reduced monosodium glutathione or dithiothreitol Klebsiella aerogenes
EDTA enzyme is inactive in absence either EDTA or a thiol such as reduced monosodium glutathione or dithiothreitol Klebsiella aerogenes
Monosodium glutathione enzyme is inactive in absence either EDTA or a thiol such as reduced monosodium glutathione or dithiothreitol Klebsiella aerogenes

General Stability

General Stability Organism
D-xylulose, ATP, glycerol and EDTA stabilize Klebsiella aerogenes

Inhibitors

Inhibitors Comment Organism Structure
Mg2+ above 0.08 mM Klebsiella aerogenes

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ MgCl2 required, optimal concentration: 0.05 mM Klebsiella aerogenes
Mn2+ 90% as effective as Mg2+ in activation Klebsiella aerogenes
Ni2+ activation, half as effective as Mg2+ Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-xylulose Klebsiella aerogenes enzyme of ribitol catabolism, inducible by ribitol ?
-
?

Organism

Organism UniProt Comment Textmining
Klebsiella aerogenes
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Klebsiella aerogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
95
-
-
Klebsiella aerogenes

Storage Stability

Storage Stability Organism
-20°C, about 30% of original activity retained after 4 years Klebsiella aerogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-xylulose enzyme of ribitol catabolism, inducible by ribitol Klebsiella aerogenes ?
-
?
ATP + D-xylulose specific for D-isomer Klebsiella aerogenes ADP + D-xylulose 5-phosphate
-
r
ATP + xylitol poor substrate Klebsiella aerogenes ADP + ?
-
?
GTP + D-xylulose phosphorylation at 25% the rate of ATP Klebsiella aerogenes GDP + D-xylulose 5-phosphate
-
?
ITP + D-xylulose phosphorylation at 25% the rate of ATP Klebsiella aerogenes IDP + D-xylulose 5-phosphate
-
?
UTP + D-xylulose phosphorylation at 14% the rate of ATP Klebsiella aerogenes UDP + D-xylulose 5-phosphate
-
?