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Literature summary for 2.7.1.17 extracted from

  • Neuberger, M.S.; Hartley, B.S.; Walker, J.E.
    Purification and properties of D-ribulokinase and D-xylulokinase from Klebsiella aerogenes (1981), Biochem. J., 193, 513-524.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli K12 strain NC260 harbouring multi-copy lambdaprbt-101 dal-201 prophage Klebsiella aerogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
D-xylulose
-
Klebsiella aerogenes

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Klebsiella aerogenes 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
2 * 54000, SDS-PAGE Klebsiella aerogenes
110000
-
gel filtration Klebsiella aerogenes

Organism

Organism UniProt Comment Textmining
Klebsiella aerogenes
-
expressed as constitutive enzyme in Escherichia coli K12 construct strain NC260
-

Purification (Commentary)

Purification (Comment) Organism
-
Klebsiella aerogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-xylulose
-
Klebsiella aerogenes ADP + D-xylulose 5-phosphate
-
r

Subunits

Subunits Comment Organism
dimer 2 * 54000, SDS-PAGE Klebsiella aerogenes

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
inactivation, only partially reversible by incubation at 37°C Klebsiella aerogenes