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Literature summary for 2.7.1.165 extracted from
- Production of d-Glyceric acid from d-Galacturonate in Escherichia coli (2020), J. Ind. Microbiol. Biotechnol., 47, 1075-1081 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | a microbial production platform in Escherichia coli to synthesize D-glyceric acid from D-galacturonate represents an alternative for the production of D-glyceric acid, an industrially relevant chemical, that addresses current challenges in using acetic acid bacteria for its synthesis: increasing yield, enantio-purity and biological stability, overview | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | development of a microbial production platform in Escherichia coli to synthesize D-glyceric acid from D-galacturonate. The expression of uronate dehydrogenase (udh) from Pseudomonas syringae and galactarolactone isomerase (gli) from Agrobacterium fabrum, along with the inactivation of garK, encoding for glycerate kinase, enables D-glyceric acid accumulation by utilizing the endogenous expression of galactarate dehydratase (garD), 5-keto-4-deoxy-D-glucarate aldolase (garL), and 2-hydroxy-3-oxopropionate reductase (garR). Optimization of carbon flux through the elimination of competing metabolic pathways leads to the development of a DELTAgarKDELTAhyiDELTAglxKDELTAuxaC mutant strain that produces 4.8 g/l of D-glyceric acid from D-galacturonate, with an 83% molar yield. Cultivation in a minimal medium produces similar yields and demonstrates that galactose or glycerol serve as possible carbon co-feeds for industrial production, method overview | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-glycerate | Escherichia coli | - |
ADP + 2-phospho-(R)-glycerate | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P23524 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-glycerate | - |
Escherichia coli | ADP + 2-phospho-(R)-glycerate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| garK | - |
Escherichia coli |
| glycerate kinase | - |
Escherichia coli |
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