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Literature summary for 2.7.1.165 extracted from

  • Liu, B.; Wu, L.; Liu, T.; Hong, Y.; Shen, Y.; Ni, J.
    A MOFRL family glycerate kinase from the thermophilic crenarchaeon, Sulfolobus tokodaii, with unique enzymatic properties (2009), Biotechnol. Lett., 31, 1937-1941.
    View publication on PubMed
Search for more literature for 2.7.1.165

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Sulfurisphaera tokodaii
expressed in Escherichia coli BL21(DE3) cells Sulfurisphaera tokodaii

General Stability

General Stability Organism
resistant to the 1 mM guanidine hydrochloride (85% of the original activity) and 1 mM NaF (65% of original activity) relatively stable in 10 mM MnCl2, CoSO4, CaCl2, SrCl2, NiCl2, CuCl2, HgCl2 Sulfurisphaera tokodaii

Inhibitors

Inhibitors Comment Organism Structure
2-propanol 10% v/v, less than 65% inhibition; less than 65% activity in the presence of 10% (v/v) Sulfurisphaera tokodaii
Ca2+ 85% activity in the presence of 10 mM Ca2+ compared to Mg2+ Sulfurisphaera tokodaii
ethanol 10% v/v, less than 65% inhibition; less than 65% activity in the presence of 10% (v/v) Sulfurisphaera tokodaii
guanidine hydrochloride 85% residual activity at 1 M guanidine hydrochloride Sulfurisphaera tokodaii
Mn2+ 93% activity in the presence of 10 mM Mn2+ compared to Mg2+ Sulfurisphaera tokodaii
n-butanol 10% v/v, less than 65% inhibition; less than 65% activity in the presence of 10% (v/v) Sulfurisphaera tokodaii
Ni2+ 68% activity in the presence of 10 mM Ni2+ compared to Mg2+ Sulfurisphaera tokodaii
Phenylmethylsulfonylfluoride less than 30% residual activity at 10 mM Sulfurisphaera tokodaii
PMSF 10 mM, more than 70% inhibition Sulfurisphaera tokodaii
SDS 10 mM, more than 60% inhibition; less than 40% residual activity at 10 mM Sulfurisphaera tokodaii
Sodium fluoride 65% residual activity at 1 M sodium fluoride Sulfurisphaera tokodaii
Sr2+ 79% activity in the presence of 10 mM Sr2+ compared to Mg2+ Sulfurisphaera tokodaii
Zn2+ 84% activity in the presence of 10 mM Zn2+ compared to Mg2+ Sulfurisphaera tokodaii

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ divalent metal ion required, Ca2+ (10 mM) shows 85% of the activity compared to Mg2+ Sulfurisphaera tokodaii
Co2+ divalent metal ion required, maximal activity in presence of Co2+ or Mg2+ (10 mM) Sulfurisphaera tokodaii
Co2+ 106% activity in the presence of 10 mM Co2+ compared to Mg2+ Sulfurisphaera tokodaii
Mg2+ divalent metal ion required, maximal activity in presence of Co2+ or Mg2+ (10 mM) Sulfurisphaera tokodaii
Mg2+ 100% activity in the presence of 10 mM Sulfurisphaera tokodaii
Mn2+ divalent metal ion required, Mn2+ (10 mM) shows 93% of the activity compared to Mg2+ Sulfurisphaera tokodaii
additional information monovalent metal ions have no effect on the enzyme activity Sulfurisphaera tokodaii
Ni2+ divalent metal ion required, Ni2+ (10 mM) shows 68% of the activity compared to Mg2+ Sulfurisphaera tokodaii
Sr2+ divalent metal ion required, Sr2+ (10 mM) shows 79% of the activity compared to Mg2+ Sulfurisphaera tokodaii
Zn2+ divalent metal ion required, Zn2+ (10 mM) shows 84% of the activity compared to Mg2+ Sulfurisphaera tokodaii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 2 * 45000, SDS-PAGE Sulfurisphaera tokodaii
80000
-
 gel filtration Sulfurisphaera tokodaii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (R)-glycerate Sulfurisphaera tokodaii key enzyme in the Entner-Doudoroff pathway in archaea ADP + 2-phospho-(R)-glycerate
-
 ?
ATP + (R)-glycerate Sulfurisphaera tokodaii 7 key enzyme in the Entner-Doudoroff pathway in archaea ADP + 2-phospho-(R)-glycerate
-
 ?

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii Q96YZ3
-
-
Sulfurisphaera tokodaii 7 Q96YZ3
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Sulfurisphaera tokodaii
recombinant enzyme Sulfurisphaera tokodaii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP + (R)-glycerate 76% of the activity with GTP Sulfurisphaera tokodaii AMP + 2-phospho-(R)-glycerate
-
 ?
ADP + (R)-glycerate 76% of the activity with GTP Sulfurisphaera tokodaii 7 AMP + 2-phospho-(R)-glycerate
-
 ?
ADP + D-glycerate 76% activity compared to GTP Sulfurisphaera tokodaii AMP + 2-phospho-D-glycerate
-
 ?
AMP + (R)-glycerate 54% of the activity with GTP Sulfurisphaera tokodaii adenosine + 2-phospho-(R)-glycerate
-
 ?
AMP + D-glycerate 54% activity compared to GTP Sulfurisphaera tokodaii adenosine + 2-phospho-D-glycerate
-
 ?
ATP + (R)-glycerate key enzyme in the Entner-Doudoroff pathway in archaea Sulfurisphaera tokodaii ADP + 2-phospho-(R)-glycerate
-
 ?
ATP + (R)-glycerate 92% of the activity with GTP Sulfurisphaera tokodaii ADP + 2-phospho-(R)-glycerate
-
 ?
ATP + (R)-glycerate key enzyme in the Entner-Doudoroff pathway in archaea Sulfurisphaera tokodaii 7 ADP + 2-phospho-(R)-glycerate
-
 ?
ATP + (R)-glycerate 92% of the activity with GTP Sulfurisphaera tokodaii 7 ADP + 2-phospho-(R)-glycerate
-
 ?
ATP + D-glycerate 92% activity compared to GTP Sulfurisphaera tokodaii ADP + 2-phospho-D-glycerate
-
 ?
CTP + (R)-glycerate 87% of the activity with GTP Sulfurisphaera tokodaii CDP + 2-phospho-(R)-glycerate
-
 ?
CTP + (R)-glycerate 87% of the activity with GTP Sulfurisphaera tokodaii 7 CDP + 2-phospho-(R)-glycerate
-
 ?
CTP + D-glycerate 87% activity compared to GTP Sulfurisphaera tokodaii CDP + 2-phospho-D-glycerate
-
 ?
diphosphate + (R)-glycerate 63% of the activity with ATP Sulfurisphaera tokodaii phosphate + 2-phospho-(R)-glycerate
-
 ?
diphosphate + (R)-glycerate 63% of the activity with ATP Sulfurisphaera tokodaii 7 phosphate + 2-phospho-(R)-glycerate
-
 ?
diphosphate + D-glycerate 63% activity compared to GTP Sulfurisphaera tokodaii phosphate + 2-phospho-D-glycerate
-
 ?
GTP + (R)-glycerate
-
 Sulfurisphaera tokodaii GDP + 2-phospho-(R)-glycerate
-
 ?
GTP + D-glycerate 100% activity towards GTP Sulfurisphaera tokodaii GDP + 2-phospho-D-glycerate
-
 ?
UTP + (R)-glycerate 81% of the activity with GTP Sulfurisphaera tokodaii UDP + 2-phospho-(R)-glycerate
-
 ?
UTP + D-glycerate 81% activity compared to GTP Sulfurisphaera tokodaii UDP + 2-phospho-D-glycerate
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 45000, SDS-PAGE Sulfurisphaera tokodaii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
-
 Sulfurisphaera tokodaii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70 100
-
 Sulfurisphaera tokodaii
70 100 70°C: about 60% of maximal activity, 100°C: about 85% of maximal activity Sulfurisphaera tokodaii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
 12 h, more than 85% of the activity remains Sulfurisphaera tokodaii
100
-
 the enzyme activity shows no obvious decrease even after heat-treatment at 100°C for 12 h and most of the optimum activity (more than 85%) remains Sulfurisphaera tokodaii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Sulfurisphaera tokodaii

pH Range

pH Minimum pH Maximum Comment Organism
3 9 pH 3: about 85% of maximal activity, pH 9.0: about 50% of maximal activity Sulfurisphaera tokodaii

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Sulfurisphaera tokodaii