BRENDA - Enzyme Database show
show all sequences of 2.7.1.165

A MOFRL family glycerate kinase from the thermophilic crenarchaeon, Sulfolobus tokodaii, with unique enzymatic properties

Liu, B.; Wu, L.; Liu, T.; Hong, Y.; Shen, Y.; Ni, J.; Biotechnol. Lett. 31, 1937-1941 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli; expressed in Escherichia coli BL21(DE3) cells
Sulfurisphaera tokodaii
General Stability
General Stability
Organism
resistant to the 1 mM guanidine hydrochloride (85% of the original activity) and 1 mM NaF (65% of original activity) relatively stable in 10 mM MnCl2, CoSO4, CaCl2, SrCl2, NiCl2, CuCl2, HgCl2
Sulfurisphaera tokodaii
Inhibitors
Inhibitors
Commentary
Organism
Structure
2-propanol
10% v/v, less than 65% inhibition; less than 65% activity in the presence of 10% (v/v)
Sulfurisphaera tokodaii
Ca2+
85% activity in the presence of 10 mM Ca2+ compared to Mg2+
Sulfurisphaera tokodaii
ethanol
10% v/v, less than 65% inhibition; less than 65% activity in the presence of 10% (v/v)
Sulfurisphaera tokodaii
guanidine hydrochloride
85% residual activity at 1 M guanidine hydrochloride
Sulfurisphaera tokodaii
Mn2+
93% activity in the presence of 10 mM Mn2+ compared to Mg2+
Sulfurisphaera tokodaii
n-butanol
10% v/v, less than 65% inhibition; less than 65% activity in the presence of 10% (v/v)
Sulfurisphaera tokodaii
Ni2+
68% activity in the presence of 10 mM Ni2+ compared to Mg2+
Sulfurisphaera tokodaii
Phenylmethylsulfonylfluoride
less than 30% residual activity at 10 mM
Sulfurisphaera tokodaii
PMSF
10 mM, more than 70% inhibition
Sulfurisphaera tokodaii
SDS
10 mM, more than 60% inhibition; less than 40% residual activity at 10 mM
Sulfurisphaera tokodaii
Sodium fluoride
65% residual activity at 1 M sodium fluoride
Sulfurisphaera tokodaii
Sr2+
79% activity in the presence of 10 mM Sr2+ compared to Mg2+
Sulfurisphaera tokodaii
Zn2+
84% activity in the presence of 10 mM Zn2+ compared to Mg2+
Sulfurisphaera tokodaii
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
divalent metal ion required, Ca2+ (10 mM) shows 85% of the activity compared to Mg2+
Sulfurisphaera tokodaii
Co2+
106% activity in the presence of 10 mM Co2+ compared to Mg2+; divalent metal ion required, maximal activity in presence of Co2+ or Mg2+ (10 mM)
Sulfurisphaera tokodaii
Mg2+
100% activity in the presence of 10 mM; divalent metal ion required, maximal activity in presence of Co2+ or Mg2+ (10 mM)
Sulfurisphaera tokodaii
Mn2+
divalent metal ion required, Mn2+ (10 mM) shows 93% of the activity compared to Mg2+
Sulfurisphaera tokodaii
additional information
monovalent metal ions have no effect on the enzyme activity
Sulfurisphaera tokodaii
Ni2+
divalent metal ion required, Ni2+ (10 mM) shows 68% of the activity compared to Mg2+
Sulfurisphaera tokodaii
Sr2+
divalent metal ion required, Sr2+ (10 mM) shows 79% of the activity compared to Mg2+
Sulfurisphaera tokodaii
Zn2+
divalent metal ion required, Zn2+ (10 mM) shows 84% of the activity compared to Mg2+
Sulfurisphaera tokodaii
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
2 * 45000, SDS-PAGE
Sulfurisphaera tokodaii
80000
-
gel filtration
Sulfurisphaera tokodaii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + (R)-glycerate
Sulfurisphaera tokodaii
key enzyme in the Entner-Doudoroff pathway in archaea
ADP + 2-phospho-(R)-glycerate
-
-
?
ATP + (R)-glycerate
Sulfurisphaera tokodaii 7
key enzyme in the Entner-Doudoroff pathway in archaea
ADP + 2-phospho-(R)-glycerate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sulfurisphaera tokodaii
Q96YZ3
-
-
Sulfurisphaera tokodaii 7
Q96YZ3
-
-
Purification (Commentary)
Commentary
Organism
Ni-NTA column chromatography; recombinant enzyme
Sulfurisphaera tokodaii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ADP + (R)-glycerate
76% of the activity with GTP
696906
Sulfurisphaera tokodaii
AMP + 2-phospho-(R)-glycerate
-
-
-
?
ADP + (R)-glycerate
76% of the activity with GTP
696906
Sulfurisphaera tokodaii 7
AMP + 2-phospho-(R)-glycerate
-
-
-
?
ADP + D-glycerate
76% activity compared to GTP
696906
Sulfurisphaera tokodaii
AMP + 2-phospho-D-glycerate
-
-
-
?
AMP + (R)-glycerate
54% of the activity with GTP
696906
Sulfurisphaera tokodaii
adenosine + 2-phospho-(R)-glycerate
-
-
-
?
AMP + D-glycerate
54% activity compared to GTP
696906
Sulfurisphaera tokodaii
adenosine + 2-phospho-D-glycerate
-
-
-
?
ATP + (R)-glycerate
key enzyme in the Entner-Doudoroff pathway in archaea
696906
Sulfurisphaera tokodaii
ADP + 2-phospho-(R)-glycerate
-
-
-
?
ATP + (R)-glycerate
92% of the activity with GTP
696906
Sulfurisphaera tokodaii
ADP + 2-phospho-(R)-glycerate
-
-
-
?
ATP + (R)-glycerate
key enzyme in the Entner-Doudoroff pathway in archaea
696906
Sulfurisphaera tokodaii 7
ADP + 2-phospho-(R)-glycerate
-
-
-
?
ATP + (R)-glycerate
92% of the activity with GTP
696906
Sulfurisphaera tokodaii 7
ADP + 2-phospho-(R)-glycerate
-
-
-
?
ATP + D-glycerate
92% activity compared to GTP
696906
Sulfurisphaera tokodaii
ADP + 2-phospho-D-glycerate
-
-
-
?
CTP + (R)-glycerate
87% of the activity with GTP
696906
Sulfurisphaera tokodaii
CDP + 2-phospho-(R)-glycerate
-
-
-
?
CTP + (R)-glycerate
87% of the activity with GTP
696906
Sulfurisphaera tokodaii 7
CDP + 2-phospho-(R)-glycerate
-
-
-
?
CTP + D-glycerate
87% activity compared to GTP
696906
Sulfurisphaera tokodaii
CDP + 2-phospho-D-glycerate
-
-
-
?
diphosphate + (R)-glycerate
63% of the activity with ATP
696906
Sulfurisphaera tokodaii
phosphate + 2-phospho-(R)-glycerate
-
-
-
?
diphosphate + (R)-glycerate
63% of the activity with ATP
696906
Sulfurisphaera tokodaii 7
phosphate + 2-phospho-(R)-glycerate
-
-
-
?
diphosphate + D-glycerate
63% activity compared to GTP
696906
Sulfurisphaera tokodaii
phosphate + 2-phospho-D-glycerate
-
-
-
?
GTP + (R)-glycerate
-
696906
Sulfurisphaera tokodaii
GDP + 2-phospho-(R)-glycerate
-
-
-
?
GTP + D-glycerate
100% activity towards GTP
696906
Sulfurisphaera tokodaii
GDP + 2-phospho-D-glycerate
-
-
-
?
UTP + (R)-glycerate
81% of the activity with GTP
696906
Sulfurisphaera tokodaii
UDP + 2-phospho-(R)-glycerate
-
-
-
?
UTP + D-glycerate
81% activity compared to GTP
696906
Sulfurisphaera tokodaii
UDP + 2-phospho-D-glycerate
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
2 * 45000, SDS-PAGE
Sulfurisphaera tokodaii
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
90
-
-
Sulfurisphaera tokodaii
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
70
100
; 70°C: about 60% of maximal activity, 100°C: about 85% of maximal activity
Sulfurisphaera tokodaii
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
100
-
12 h, more than 85% of the activity remains; the enzyme activity shows no obvious decrease even after heat-treatment at 100°C for 12 h and most of the optimum activity (more than 85%) remains
Sulfurisphaera tokodaii
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.5
-
-
Sulfurisphaera tokodaii
pH Range
pH Minimum
pH Maximum
Commentary
Organism
3
9
pH 3: about 85% of maximal activity, pH 9.0: about 50% of maximal activity
Sulfurisphaera tokodaii
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
-
Sulfurisphaera tokodaii
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli; expressed in Escherichia coli BL21(DE3) cells
Sulfurisphaera tokodaii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Sulfurisphaera tokodaii
General Stability (protein specific)
General Stability
Organism
resistant to the 1 mM guanidine hydrochloride (85% of the original activity) and 1 mM NaF (65% of original activity) relatively stable in 10 mM MnCl2, CoSO4, CaCl2, SrCl2, NiCl2, CuCl2, HgCl2
Sulfurisphaera tokodaii
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-propanol
10% v/v, less than 65% inhibition; less than 65% activity in the presence of 10% (v/v)
Sulfurisphaera tokodaii
Ca2+
85% activity in the presence of 10 mM Ca2+ compared to Mg2+
Sulfurisphaera tokodaii
ethanol
10% v/v, less than 65% inhibition; less than 65% activity in the presence of 10% (v/v)
Sulfurisphaera tokodaii
guanidine hydrochloride
85% residual activity at 1 M guanidine hydrochloride
Sulfurisphaera tokodaii
Mn2+
93% activity in the presence of 10 mM Mn2+ compared to Mg2+
Sulfurisphaera tokodaii
n-butanol
10% v/v, less than 65% inhibition; less than 65% activity in the presence of 10% (v/v)
Sulfurisphaera tokodaii
Ni2+
68% activity in the presence of 10 mM Ni2+ compared to Mg2+
Sulfurisphaera tokodaii
Phenylmethylsulfonylfluoride
less than 30% residual activity at 10 mM
Sulfurisphaera tokodaii
PMSF
10 mM, more than 70% inhibition
Sulfurisphaera tokodaii
SDS
10 mM, more than 60% inhibition; less than 40% residual activity at 10 mM
Sulfurisphaera tokodaii
Sodium fluoride
65% residual activity at 1 M sodium fluoride
Sulfurisphaera tokodaii
Sr2+
79% activity in the presence of 10 mM Sr2+ compared to Mg2+
Sulfurisphaera tokodaii
Zn2+
84% activity in the presence of 10 mM Zn2+ compared to Mg2+
Sulfurisphaera tokodaii
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
divalent metal ion required, Ca2+ (10 mM) shows 85% of the activity compared to Mg2+
Sulfurisphaera tokodaii
Co2+
106% activity in the presence of 10 mM Co2+ compared to Mg2+; divalent metal ion required, maximal activity in presence of Co2+ or Mg2+ (10 mM)
Sulfurisphaera tokodaii
Mg2+
100% activity in the presence of 10 mM; divalent metal ion required, maximal activity in presence of Co2+ or Mg2+ (10 mM)
Sulfurisphaera tokodaii
Mn2+
divalent metal ion required, Mn2+ (10 mM) shows 93% of the activity compared to Mg2+
Sulfurisphaera tokodaii
additional information
monovalent metal ions have no effect on the enzyme activity
Sulfurisphaera tokodaii
Ni2+
divalent metal ion required, Ni2+ (10 mM) shows 68% of the activity compared to Mg2+
Sulfurisphaera tokodaii
Sr2+
divalent metal ion required, Sr2+ (10 mM) shows 79% of the activity compared to Mg2+
Sulfurisphaera tokodaii
Zn2+
divalent metal ion required, Zn2+ (10 mM) shows 84% of the activity compared to Mg2+
Sulfurisphaera tokodaii
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
2 * 45000, SDS-PAGE
Sulfurisphaera tokodaii
80000
-
gel filtration
Sulfurisphaera tokodaii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + (R)-glycerate
Sulfurisphaera tokodaii
key enzyme in the Entner-Doudoroff pathway in archaea
ADP + 2-phospho-(R)-glycerate
-
-
?
ATP + (R)-glycerate
Sulfurisphaera tokodaii 7
key enzyme in the Entner-Doudoroff pathway in archaea
ADP + 2-phospho-(R)-glycerate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA column chromatography; recombinant enzyme
Sulfurisphaera tokodaii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ADP + (R)-glycerate
76% of the activity with GTP
696906
Sulfurisphaera tokodaii
AMP + 2-phospho-(R)-glycerate
-
-
-
?
ADP + (R)-glycerate
76% of the activity with GTP
696906
Sulfurisphaera tokodaii 7
AMP + 2-phospho-(R)-glycerate
-
-
-
?
ADP + D-glycerate
76% activity compared to GTP
696906
Sulfurisphaera tokodaii
AMP + 2-phospho-D-glycerate
-
-
-
?
AMP + (R)-glycerate
54% of the activity with GTP
696906
Sulfurisphaera tokodaii
adenosine + 2-phospho-(R)-glycerate
-
-
-
?
AMP + D-glycerate
54% activity compared to GTP
696906
Sulfurisphaera tokodaii
adenosine + 2-phospho-D-glycerate
-
-
-
?
ATP + (R)-glycerate
key enzyme in the Entner-Doudoroff pathway in archaea
696906
Sulfurisphaera tokodaii
ADP + 2-phospho-(R)-glycerate
-
-
-
?
ATP + (R)-glycerate
92% of the activity with GTP
696906
Sulfurisphaera tokodaii
ADP + 2-phospho-(R)-glycerate
-
-
-
?
ATP + (R)-glycerate
key enzyme in the Entner-Doudoroff pathway in archaea
696906
Sulfurisphaera tokodaii 7
ADP + 2-phospho-(R)-glycerate
-
-
-
?
ATP + (R)-glycerate
92% of the activity with GTP
696906
Sulfurisphaera tokodaii 7
ADP + 2-phospho-(R)-glycerate
-
-
-
?
ATP + D-glycerate
92% activity compared to GTP
696906
Sulfurisphaera tokodaii
ADP + 2-phospho-D-glycerate
-
-
-
?
CTP + (R)-glycerate
87% of the activity with GTP
696906
Sulfurisphaera tokodaii
CDP + 2-phospho-(R)-glycerate
-
-
-
?
CTP + (R)-glycerate
87% of the activity with GTP
696906
Sulfurisphaera tokodaii 7
CDP + 2-phospho-(R)-glycerate
-
-
-
?
CTP + D-glycerate
87% activity compared to GTP
696906
Sulfurisphaera tokodaii
CDP + 2-phospho-D-glycerate
-
-
-
?
diphosphate + (R)-glycerate
63% of the activity with ATP
696906
Sulfurisphaera tokodaii
phosphate + 2-phospho-(R)-glycerate
-
-
-
?
diphosphate + (R)-glycerate
63% of the activity with ATP
696906
Sulfurisphaera tokodaii 7
phosphate + 2-phospho-(R)-glycerate
-
-
-
?
diphosphate + D-glycerate
63% activity compared to GTP
696906
Sulfurisphaera tokodaii
phosphate + 2-phospho-D-glycerate
-
-
-
?
GTP + (R)-glycerate
-
696906
Sulfurisphaera tokodaii
GDP + 2-phospho-(R)-glycerate
-
-
-
?
GTP + D-glycerate
100% activity towards GTP
696906
Sulfurisphaera tokodaii
GDP + 2-phospho-D-glycerate
-
-
-
?
UTP + (R)-glycerate
81% of the activity with GTP
696906
Sulfurisphaera tokodaii
UDP + 2-phospho-(R)-glycerate
-
-
-
?
UTP + D-glycerate
81% activity compared to GTP
696906
Sulfurisphaera tokodaii
UDP + 2-phospho-D-glycerate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 45000, SDS-PAGE
Sulfurisphaera tokodaii
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
90
-
-
Sulfurisphaera tokodaii
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
70
100
; 70°C: about 60% of maximal activity, 100°C: about 85% of maximal activity
Sulfurisphaera tokodaii
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
100
-
12 h, more than 85% of the activity remains; the enzyme activity shows no obvious decrease even after heat-treatment at 100°C for 12 h and most of the optimum activity (more than 85%) remains
Sulfurisphaera tokodaii
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.5
-
-
Sulfurisphaera tokodaii
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
3
9
pH 3: about 85% of maximal activity, pH 9.0: about 50% of maximal activity
Sulfurisphaera tokodaii
Other publictions for EC 2.7.1.165
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739276
Keech
The redox control of photoresp ...
Arabidopsis thaliana, Zea mays
Plant Cell Environ.
40
553-569
2017
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739413
Zelcbuch
An in vivo metabolic approach ...
Escherichia coli
PLoS ONE
10
e0122957
2015
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722247
Kouril
Unraveling the function of the ...
Saccharolobus solfataricus, Saccharolobus solfataricus P2
FEBS J.
280
1126-1138
2013
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1
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2
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5
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1
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1
1
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1
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4
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1
1
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722379
Sass
D-glyceric aciduria is caused ...
Homo sapiens
Hum. Mutat.
31
1280-1285
2010
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1
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3
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696906
Liu
A MOFRL family glycerate kinas ...
Sulfurisphaera tokodaii, Sulfurisphaera tokodaii 7
Biotechnol. Lett.
31
1937-1941
2009
-
-
1
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