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Literature summary for 2.7.1.164 extracted from

  • Sherrer, R.L.; O'Donoghue, P.; Söll D.
    Characterization and evolutionary history of an archaeal kinase involved in selenocysteinyl-tRNA formation (2008), Nucleic Acids Res., 36, 1247-1259.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Methanocaldococcus jannaschii

Protein Variants

Protein Variants Comment Organism
D41A strongly reduced activity Methanocaldococcus jannaschii
G14W strongly reduced activity Methanocaldococcus jannaschii
K17A strongly reduced activity Methanocaldococcus jannaschii
R116A mutant enzyme is 23.5fold less active than wild-type enzyme Methanocaldococcus jannaschii
R120A strongly reduced activity Methanocaldococcus jannaschii
S18A strongly reduced activity Methanocaldococcus jannaschii
T19W mutant enzyme is 2.8fold less active than wild-type enzyme Methanocaldococcus jannaschii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00004
-
L-seryl-tRNASec 37°C Methanocaldococcus jannaschii
2.6
-
ATP 37°C Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-seryl-tRNASec Methanocaldococcus jannaschii
-
ADP + O-phospho-L-seryl-tRNASec
-
?
ATP + L-seryl-tRNASec Methanocaldococcus jannaschii DSM 2661
-
ADP + O-phospho-L-seryl-tRNASec
-
?

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q58933 MJ1538
-
Methanocaldococcus jannaschii DSM 2661 Q58933 MJ1538
-

Purification (Commentary)

Purification (Comment) Organism
-
Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-seryl-tRNASec
-
Methanocaldococcus jannaschii ADP + O-phospho-L-seryl-tRNASec
-
?
ATP + L-seryl-tRNASec the enzyme does not recognize free Ser as a substrate for phosphate transfer. Ser attached to tRNASec is its obligate substrate. Neither tRNASer nor L-seryl-tRNASer is a substrate for phosphorylation Methanocaldococcus jannaschii ADP + O-phospho-L-seryl-tRNASec
-
?
ATP + L-seryl-tRNASec
-
Methanocaldococcus jannaschii DSM 2661 ADP + O-phospho-L-seryl-tRNASec
-
?
ATP + L-seryl-tRNASec the enzyme does not recognize free Ser as a substrate for phosphate transfer. Ser attached to tRNASec is its obligate substrate. Neither tRNASer nor L-seryl-tRNASer is a substrate for phosphorylation Methanocaldococcus jannaschii DSM 2661 ADP + O-phospho-L-seryl-tRNASec
-
?
CTP + L-seryl-tRNASec phosphorylation at about 60% the activity of ATP Methanocaldococcus jannaschii CDP + O-phospho-L-seryl-tRNASec
-
?
CTP + L-seryl-tRNASec phosphorylation at about 60% the activity of ATP Methanocaldococcus jannaschii DSM 2661 CDP + O-phospho-L-seryl-tRNASec
-
?
dATP + L-seryl-tRNASec phosphorylation at about 65% the activity of ATP Methanocaldococcus jannaschii dADP + O-phospho-L-seryl-tRNASec
-
?
dATP + L-seryl-tRNASec phosphorylation at about 65% the activity of ATP Methanocaldococcus jannaschii DSM 2661 dADP + O-phospho-L-seryl-tRNASec
-
?
GTP + L-seryl-tRNASec phosphorylation at about 40% the activity of ATP Methanocaldococcus jannaschii GDP + O-phospho-L-seryl-tRNASec
-
?
ITP + L-seryl-tRNASec phosphorylation at about 85% the activity of ATP Methanocaldococcus jannaschii IDP + O-phospho-L-seryl-tRNASec
-
?
additional information no phosphorylation activity with 5'-adenylyl (beta,gamma-methylene)diphosphonate, very low phosphorylation activity with alpha,beta-methyleneadenosine 5'-triphosphate Methanocaldococcus jannaschii ?
-
?
additional information no phosphorylation activity with 5'-adenylyl (beta,gamma-methylene)diphosphonate, very low phosphorylation activity with alpha,beta-methyleneadenosine 5'-triphosphate Methanocaldococcus jannaschii DSM 2661 ?
-
?
UTP + L-seryl-tRNASec phosphorylation at about 40% the activity of ATP Methanocaldococcus jannaschii UDP + O-phospho-L-seryl-tRNASec
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.069
-
ATP 37°C Methanocaldococcus jannaschii
0.098
-
L-seryl-tRNASec 37°C Methanocaldococcus jannaschii