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Literature summary for 2.7.1.162 extracted from
- Identification of N-acetylhexosamine 1-kinase in the complete lacto-N-biose I/galacto-N-biose metabolic pathway in Bifidobacterium longum (2007), Appl. Environ. Microbiol., 73, 6444-6449.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| isopropyl-1-thio-beta-D-galactoside | induction of protein expression at a final concentration of 0.5 mM | Bifidobacterium longum |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| Escherichia coli strains select96 and BL21(DE3) are used as hosts for cloning and expression. | Bifidobacterium longum |
| expression in Escherichia coli | Bifidobacterium longum |
General Stability
| General Stability | Organism |
|---|---|
| loses half of enzyme activity during incubation for 30 min at 50°C in 0.1 M Tris buffer (pH 8.5) | Bifidobacterium longum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.065 | - |
N-acetyl-alpha-D-galactosamine | - |
Bifidobacterium longum | |
| 0.065 | - |
N-acetyl-alpha-D-galactosamine | pH 8.5, 30°C | Bifidobacterium longum | |
| 0.118 | - |
N-acetyl-alpha-D-glucosamine | - |
Bifidobacterium longum | |
| 0.118 | - |
N-acetyl-alpha-D-glucosamine | pH 8.5, 30°C | Bifidobacterium longum | |
| 0.172 | - |
ATP | cosubstrate: N-acetyl-alpha-D-glucosamine, pH 8.5, 30°C | Bifidobacterium longum | |
| 0.172 | - |
ATP | at N-acetyl-alpha-D-glucosamine saturation | Bifidobacterium longum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM, 1% relative activity compared to Mg2+ | Bifidobacterium longum | |
| Co2+ | 21% of the activity with Mg2+ | Bifidobacterium longum | |
| Co2+ | 1 mM, 21% relative activity compared to Mg2+ | Bifidobacterium longum | |
| Mg2+ | requires a divalent cation, with Mg2+ resulting in the greatest stimulation of enzyme activity | Bifidobacterium longum | |
| Mg2+ | 1 mM, 100% relative activity | Bifidobacterium longum | |
| Mn2+ | 1 mM, 7% relative activity compared to Mg2+ | Bifidobacterium longum | |
| Ni2+ | 1 mM, 4% relative activity compared to Mg2+ | Bifidobacterium longum | |
| Zn2+ | 1 mM, 8% relative activity compared to Mg2+ | Bifidobacterium longum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + N-acetyl-alpha-D-galactosamine | Bifidobacterium longum | the enzyme is involved in the lacto-N-biose I/galacto-N-biose degradation pathway | ADP + N-acetyl-alpha-D-galactosamine 1-phosphate | - |
? | |
| ATP + N-acetyl-alpha-D-galactosamine | Bifidobacterium longum | lacto-N-biose I/galacto-N-biose metabolic pathway | ADP + N-acetyl-alpha-D-galactosamine 1-phosphate | - |
? | |
| ATP + N-acetyl-alpha-D-galactosamine | Bifidobacterium longum JCM 1217 | the enzyme is involved in the lacto-N-biose I/galacto-N-biose degradation pathway | ADP + N-acetyl-alpha-D-galactosamine 1-phosphate | - |
? | |
| ATP + N-acetyl-alpha-D-galactosamine | Bifidobacterium longum JCM 1217 | lacto-N-biose I/galacto-N-biose metabolic pathway | ADP + N-acetyl-alpha-D-galactosamine 1-phosphate | - |
? | |
| ATP + N-acetyl-alpha-D-glucosamine | Bifidobacterium longum | - |
ADP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? | |
| ATP + N-acetyl-alpha-D-glucosamine | Bifidobacterium longum | the enzyme is involved in the lacto-N-biose I/galacto-N-biose degradation pathway | ADP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bifidobacterium longum | E8MF12 | strain JCM1217 | - |
| Bifidobacterium longum JCM 1217 | E8MF12 | strain JCM1217 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bifidobacterium longum |
| The transformant is cultivated and protein expression is induced by the addition of isopropyl-1-thio-beta-D-galactoside. The cells are harvested by centrifugation and sonicated. Cell debris is removed by centrifugation and enzyme is purified on an Ni-nitrilotriacetic acid agarose gel. | Bifidobacterium longum |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + N-acetyl-D-hexosamine = ADP + N-acetyl-alpha-D-hexosamine 1-phosphate | enzyme acts by a sequential bi bi (two substrates-two products) mechanism, with the reaction occurring after the binding of both ATP and N-acetylhexosamine | Bifidobacterium longum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | strain JCM1217 | Bifidobacterium longum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
Incubation of 4 microM LnpB protein with 10 mM N-acetyl-alpha-D-glucosamine 6-sulfate in 0.1 M MOPS buffer (pH 7.5) at 30°C for 30 min shows that the protein have no hydrolytic activity | Bifidobacterium longum |
| 0.001 | - |
D-glucose, substrate donor specificity | Bifidobacterium longum |
| 0.002 | - |
D-mannosamine, substrate donor specificity | Bifidobacterium longum |
| 0.004 | - |
D-glucosamine, substrate donor specificity | Bifidobacterium longum |
| 0.007 | - |
2-deoxyglucose, substrate donor specificity | Bifidobacterium longum |
| 0.024 | - |
D-mannose, substrate donor specificity | Bifidobacterium longum |
| 0.049 | - |
D-talose, substrate donor specificity | Bifidobacterium longum |
| 0.22 | - |
N-acetyl-D-mannosamine, substrate donor specificity | Bifidobacterium longum |
| 0.48 | - |
ITP, substrate donor specificity | Bifidobacterium longum |
| 0.65 | - |
GTP, substrate donor specificity | Bifidobacterium longum |
| 0.89 | - |
N-acetyl-D-galactosamine, substrate donor specificity | Bifidobacterium longum |
| 1.48 | - |
ATP, substrate donor specificity | Bifidobacterium longum |
| 1.49 | - |
N-acetyl-D-glucosamine, substrate donor specificity | Bifidobacterium longum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-mannose | phosphorylation at 1.6% of the activity with N-acetylglucosamine | Bifidobacterium longum | ? | - |
? | |
| ATP + D-mannose | phosphorylation at 1.6% of the activity with N-acetylglucosamine | Bifidobacterium longum JCM 1217 | ? | - |
? | |
| ATP + D-talose | phosphorylation at 3% of the activity with N-acetylglucosamine | Bifidobacterium longum | ? | - |
? | |
| ATP + N-acetyl-alpha-D-galactosamine | the enzyme is involved in the lacto-N-biose I/galacto-N-biose degradation pathway | Bifidobacterium longum | ADP + N-acetyl-alpha-D-galactosamine 1-phosphate | - |
? | |
| ATP + N-acetyl-alpha-D-galactosamine | phosphorylation at 60% of the activity with N-acetylglucosamine | Bifidobacterium longum | ADP + N-acetyl-alpha-D-galactosamine 1-phosphate | - |
? | |
| ATP + N-acetyl-alpha-D-galactosamine | lacto-N-biose I/galacto-N-biose metabolic pathway | Bifidobacterium longum | ADP + N-acetyl-alpha-D-galactosamine 1-phosphate | - |
? | |
| ATP + N-acetyl-alpha-D-galactosamine | the enzyme is involved in the lacto-N-biose I/galacto-N-biose degradation pathway | Bifidobacterium longum JCM 1217 | ADP + N-acetyl-alpha-D-galactosamine 1-phosphate | - |
? | |
| ATP + N-acetyl-alpha-D-galactosamine | phosphorylation at 60% of the activity with N-acetylglucosamine | Bifidobacterium longum JCM 1217 | ADP + N-acetyl-alpha-D-galactosamine 1-phosphate | - |
? | |
| ATP + N-acetyl-alpha-D-galactosamine | lacto-N-biose I/galacto-N-biose metabolic pathway | Bifidobacterium longum JCM 1217 | ADP + N-acetyl-alpha-D-galactosamine 1-phosphate | - |
? | |
| ATP + N-acetyl-alpha-D-glucosamine | - |
Bifidobacterium longum | ADP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? | |
| ATP + N-acetyl-alpha-D-glucosamine | the enzyme is involved in the lacto-N-biose I/galacto-N-biose degradation pathway | Bifidobacterium longum | ADP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? | |
| ATP + N-acetyl-D-mannosamine | phosphorylation at 15% of the activity with N-acetylglucosamine | Bifidobacterium longum | ? | - |
? | |
| ATP + N-acetyl-D-mannosamine | - |
Bifidobacterium longum | ADP + ? | - |
? | |
| GTP + N-acetyl-alpha-D-glucosamine | GTP shows about 45% of the activity with ATP | Bifidobacterium longum | GDP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? | |
| ITP + N-acetyl-alpha-D-glucosamine | ITP shows about 30% of the activity with ATP | Bifidobacterium longum | IDP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? | |
| additional information | slightly active with D-talose, D-mannose, GTP, ITP | Bifidobacterium longum | ? | - |
? | |
| additional information | slightly active with D-talose, D-mannose, GTP, ITP | Bifidobacterium longum JCM 1217 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| lnpB | - |
Bifidobacterium longum |
| lnpB | protein | Bifidobacterium longum |
| N-acetylhexosamine 1-kinase | - |
Bifidobacterium longum |
| NahK | - |
Bifidobacterium longum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Bifidobacterium longum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
pH 8.5, 30 min, 50% loss of activity | Bifidobacterium longum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.752 | - |
N-acetyl-alpha-D-galactosamine | - |
Bifidobacterium longum | |
| 1.21 | - |
N-acetyl-alpha-D-glucosamine | - |
Bifidobacterium longum | |
| 1.21 | - |
N-acetyl-alpha-D-glucosamine | pH 8.5, 30°C | Bifidobacterium longum | |
| 6.08 | - |
N-acetyl-alpha-D-galactosamine | pH 8.5, 30°C | Bifidobacterium longum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Bifidobacterium longum |
| 8.5 | - |
assay at | Bifidobacterium longum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9.5 | stable | Bifidobacterium longum |
| 5 | 9.5 | stability at 30°C | Bifidobacterium longum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Bifidobacterium longum | |
| GTP | - |
Bifidobacterium longum | |
| ITP | - |
Bifidobacterium longum | |
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