Activating Compound | Comment | Organism | Structure |
---|---|---|---|
isopropyl-1-thio-beta-D-galactoside | induction of protein expression at a final concentration of 0.5 mM | Bifidobacterium longum |
Cloned (Comment) | Organism |
---|---|
Escherichia coli strains select96 and BL21(DE3) are used as hosts for cloning and expression. | Bifidobacterium longum |
expression in Escherichia coli | Bifidobacterium longum |
General Stability | Organism |
---|---|
loses half of enzyme activity during incubation for 30 min at 50°C in 0.1 M Tris buffer (pH 8.5) | Bifidobacterium longum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.065 | - |
N-acetyl-alpha-D-galactosamine | - |
Bifidobacterium longum | |
0.065 | - |
N-acetyl-alpha-D-galactosamine | pH 8.5, 30°C | Bifidobacterium longum | |
0.118 | - |
N-acetyl-alpha-D-glucosamine | - |
Bifidobacterium longum | |
0.118 | - |
N-acetyl-alpha-D-glucosamine | pH 8.5, 30°C | Bifidobacterium longum | |
0.172 | - |
ATP | cosubstrate: N-acetyl-alpha-D-glucosamine, pH 8.5, 30°C | Bifidobacterium longum | |
0.172 | - |
ATP | at N-acetyl-alpha-D-glucosamine saturation | Bifidobacterium longum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM, 1% relative activity compared to Mg2+ | Bifidobacterium longum | |
Co2+ | 21% of the activity with Mg2+ | Bifidobacterium longum | |
Co2+ | 1 mM, 21% relative activity compared to Mg2+ | Bifidobacterium longum | |
Mg2+ | requires a divalent cation, with Mg2+ resulting in the greatest stimulation of enzyme activity | Bifidobacterium longum | |
Mg2+ | 1 mM, 100% relative activity | Bifidobacterium longum | |
Mn2+ | 1 mM, 7% relative activity compared to Mg2+ | Bifidobacterium longum | |
Ni2+ | 1 mM, 4% relative activity compared to Mg2+ | Bifidobacterium longum | |
Zn2+ | 1 mM, 8% relative activity compared to Mg2+ | Bifidobacterium longum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + N-acetyl-alpha-D-galactosamine | Bifidobacterium longum | the enzyme is involved in the lacto-N-biose I/galacto-N-biose degradation pathway | ADP + N-acetyl-alpha-D-galactosamine 1-phosphate | - |
? | |
ATP + N-acetyl-alpha-D-galactosamine | Bifidobacterium longum | lacto-N-biose I/galacto-N-biose metabolic pathway | ADP + N-acetyl-alpha-D-galactosamine 1-phosphate | - |
? | |
ATP + N-acetyl-alpha-D-galactosamine | Bifidobacterium longum JCM 1217 | the enzyme is involved in the lacto-N-biose I/galacto-N-biose degradation pathway | ADP + N-acetyl-alpha-D-galactosamine 1-phosphate | - |
? | |
ATP + N-acetyl-alpha-D-galactosamine | Bifidobacterium longum JCM 1217 | lacto-N-biose I/galacto-N-biose metabolic pathway | ADP + N-acetyl-alpha-D-galactosamine 1-phosphate | - |
? | |
ATP + N-acetyl-alpha-D-glucosamine | Bifidobacterium longum | - |
ADP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? | |
ATP + N-acetyl-alpha-D-glucosamine | Bifidobacterium longum | the enzyme is involved in the lacto-N-biose I/galacto-N-biose degradation pathway | ADP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bifidobacterium longum | E8MF12 | strain JCM1217 | - |
Bifidobacterium longum JCM 1217 | E8MF12 | strain JCM1217 | - |
Purification (Comment) | Organism |
---|---|
- |
Bifidobacterium longum |
The transformant is cultivated and protein expression is induced by the addition of isopropyl-1-thio-beta-D-galactoside. The cells are harvested by centrifugation and sonicated. Cell debris is removed by centrifugation and enzyme is purified on an Ni-nitrilotriacetic acid agarose gel. | Bifidobacterium longum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + N-acetyl-D-hexosamine = ADP + N-acetyl-alpha-D-hexosamine 1-phosphate | enzyme acts by a sequential bi bi (two substrates-two products) mechanism, with the reaction occurring after the binding of both ATP and N-acetylhexosamine | Bifidobacterium longum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | strain JCM1217 | Bifidobacterium longum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
Incubation of 4 microM LnpB protein with 10 mM N-acetyl-alpha-D-glucosamine 6-sulfate in 0.1 M MOPS buffer (pH 7.5) at 30°C for 30 min shows that the protein have no hydrolytic activity | Bifidobacterium longum |
0.001 | - |
D-glucose, substrate donor specificity | Bifidobacterium longum |
0.002 | - |
D-mannosamine, substrate donor specificity | Bifidobacterium longum |
0.004 | - |
D-glucosamine, substrate donor specificity | Bifidobacterium longum |
0.007 | - |
2-deoxyglucose, substrate donor specificity | Bifidobacterium longum |
0.024 | - |
D-mannose, substrate donor specificity | Bifidobacterium longum |
0.049 | - |
D-talose, substrate donor specificity | Bifidobacterium longum |
0.22 | - |
N-acetyl-D-mannosamine, substrate donor specificity | Bifidobacterium longum |
0.48 | - |
ITP, substrate donor specificity | Bifidobacterium longum |
0.65 | - |
GTP, substrate donor specificity | Bifidobacterium longum |
0.89 | - |
N-acetyl-D-galactosamine, substrate donor specificity | Bifidobacterium longum |
1.48 | - |
ATP, substrate donor specificity | Bifidobacterium longum |
1.49 | - |
N-acetyl-D-glucosamine, substrate donor specificity | Bifidobacterium longum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-mannose | phosphorylation at 1.6% of the activity with N-acetylglucosamine | Bifidobacterium longum | ? | - |
? | |
ATP + D-mannose | phosphorylation at 1.6% of the activity with N-acetylglucosamine | Bifidobacterium longum JCM 1217 | ? | - |
? | |
ATP + D-talose | phosphorylation at 3% of the activity with N-acetylglucosamine | Bifidobacterium longum | ? | - |
? | |
ATP + N-acetyl-alpha-D-galactosamine | the enzyme is involved in the lacto-N-biose I/galacto-N-biose degradation pathway | Bifidobacterium longum | ADP + N-acetyl-alpha-D-galactosamine 1-phosphate | - |
? | |
ATP + N-acetyl-alpha-D-galactosamine | phosphorylation at 60% of the activity with N-acetylglucosamine | Bifidobacterium longum | ADP + N-acetyl-alpha-D-galactosamine 1-phosphate | - |
? | |
ATP + N-acetyl-alpha-D-galactosamine | lacto-N-biose I/galacto-N-biose metabolic pathway | Bifidobacterium longum | ADP + N-acetyl-alpha-D-galactosamine 1-phosphate | - |
? | |
ATP + N-acetyl-alpha-D-galactosamine | the enzyme is involved in the lacto-N-biose I/galacto-N-biose degradation pathway | Bifidobacterium longum JCM 1217 | ADP + N-acetyl-alpha-D-galactosamine 1-phosphate | - |
? | |
ATP + N-acetyl-alpha-D-galactosamine | phosphorylation at 60% of the activity with N-acetylglucosamine | Bifidobacterium longum JCM 1217 | ADP + N-acetyl-alpha-D-galactosamine 1-phosphate | - |
? | |
ATP + N-acetyl-alpha-D-galactosamine | lacto-N-biose I/galacto-N-biose metabolic pathway | Bifidobacterium longum JCM 1217 | ADP + N-acetyl-alpha-D-galactosamine 1-phosphate | - |
? | |
ATP + N-acetyl-alpha-D-glucosamine | - |
Bifidobacterium longum | ADP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? | |
ATP + N-acetyl-alpha-D-glucosamine | the enzyme is involved in the lacto-N-biose I/galacto-N-biose degradation pathway | Bifidobacterium longum | ADP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? | |
ATP + N-acetyl-D-mannosamine | phosphorylation at 15% of the activity with N-acetylglucosamine | Bifidobacterium longum | ? | - |
? | |
ATP + N-acetyl-D-mannosamine | - |
Bifidobacterium longum | ADP + ? | - |
? | |
GTP + N-acetyl-alpha-D-glucosamine | GTP shows about 45% of the activity with ATP | Bifidobacterium longum | GDP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? | |
ITP + N-acetyl-alpha-D-glucosamine | ITP shows about 30% of the activity with ATP | Bifidobacterium longum | IDP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
? | |
additional information | slightly active with D-talose, D-mannose, GTP, ITP | Bifidobacterium longum | ? | - |
? | |
additional information | slightly active with D-talose, D-mannose, GTP, ITP | Bifidobacterium longum JCM 1217 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lnpB | - |
Bifidobacterium longum |
lnpB | protein | Bifidobacterium longum |
N-acetylhexosamine 1-kinase | - |
Bifidobacterium longum |
NahK | - |
Bifidobacterium longum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Bifidobacterium longum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
pH 8.5, 30 min, 50% loss of activity | Bifidobacterium longum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.752 | - |
N-acetyl-alpha-D-galactosamine | - |
Bifidobacterium longum | |
1.21 | - |
N-acetyl-alpha-D-glucosamine | - |
Bifidobacterium longum | |
1.21 | - |
N-acetyl-alpha-D-glucosamine | pH 8.5, 30°C | Bifidobacterium longum | |
6.08 | - |
N-acetyl-alpha-D-galactosamine | pH 8.5, 30°C | Bifidobacterium longum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Bifidobacterium longum |
8.5 | - |
assay at | Bifidobacterium longum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9.5 | stable | Bifidobacterium longum |
5 | 9.5 | stability at 30°C | Bifidobacterium longum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Bifidobacterium longum | |
GTP | - |
Bifidobacterium longum | |
ITP | - |
Bifidobacterium longum |