Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme in complexes with ADP and myo-Ins(1,3,4,5,6)P5, myo-InsP6, neo-InsP6, D-chiro-InsP6, or purpurogallin, sitting drop method, mixing of 5.0-7.5 mg/ml protein solution containing 2 mM ADP and 2 mM ligand with reservoir solution, containing 18 % w/v PEG 3350, 0.1 M bis-Tris propane, pH 6.5, and 2 mM MgCl2, in a 1:1 ratio, and equilibration against 0.05 ml of reservoir solution, 16°C, microseeding X-ray diffraction structure determination and analysis, molecular replacement and modeling | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,6,7,8,4'-pentahydroxyflavone | - |
Arabidopsis thaliana | |
chaetochromin | - |
Arabidopsis thaliana | |
additional information | high throughput screening of Arabidopsis thaliana inositol pentakisphosphate 2-kinase using an active site fluorescent probe, 2-FAM-InsP5 (2-O-(2-(5-fluoresceinylcarboxy)aminoethyl)-myo-inositol 1,3,4,5,6-pentakisphosphate (triethylammonium salt)), molecular docking calculations, overview | Arabidopsis thaliana | |
purpurogallin | determination of ternary structure of AtIP5 2-K with purpurogallin and ADP, overview | Arabidopsis thaliana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate | Arabidopsis thaliana | - |
ADP + 1D-myo-inositol hexakisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q93YN9 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate | - |
Arabidopsis thaliana | ADP + 1D-myo-inositol hexakisphosphate | - |
? | |
additional information | phosphotransfer from 2-FAM-InsP5 (2-O-(2-(5-fluoresceinylcarboxy)aminoethyl)-myo-inositol 1,3,4,5,6-pentakisphosphate) to ADP by AtIP5 2-K | Arabidopsis thaliana | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
AtIP5 2-K | - |
Arabidopsis thaliana |
inositol pentakisphosphate 2-kinase | - |
Arabidopsis thaliana |
IP5 2-K | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
assay at | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | disruption of inositol pentakisphosphate 2-kinase profoundly influences cellular processes | Arabidopsis thaliana |
physiological function | inositol pentakisphosphate 2-kinase catalyzes the phosphorylation of the axial 2-OH of myo-inositol 1,3,4,5,6-pentakisphosphate for de novo synthesis of myo-inositol hexakisphosphate | Arabidopsis thaliana |