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Literature summary for 2.7.1.158 extracted from

  • Whitfield, H.; Gilmartin, M.; Baker, K.; Riley, A.M.; Godage, H.Y.; Potter, B.V.L.; Hemmings, A.M.; Brearley, C.A.
    A fluorescent probe identifies active site ligands of inositol pentakisphosphate 2-kinase (2018), J. Med. Chem., 61, 8838-8846 .
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme in complexes with ADP and myo-Ins(1,3,4,5,6)P5, myo-InsP6, neo-InsP6, D-chiro-InsP6, or purpurogallin, sitting drop method, mixing of 5.0-7.5 mg/ml protein solution containing 2 mM ADP and 2 mM ligand with reservoir solution, containing 18 % w/v PEG 3350, 0.1 M bis-Tris propane, pH 6.5, and 2 mM MgCl2, in a 1:1 ratio, and equilibration against 0.05 ml of reservoir solution, 16°C, microseeding X-ray diffraction structure determination and analysis, molecular replacement and modeling Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
5,6,7,8,4'-pentahydroxyflavone
-
Arabidopsis thaliana
chaetochromin
-
Arabidopsis thaliana
additional information high throughput screening of Arabidopsis thaliana inositol pentakisphosphate 2-kinase using an active site fluorescent probe, 2-FAM-InsP5 (2-O-(2-(5-fluoresceinylcarboxy)aminoethyl)-myo-inositol 1,3,4,5,6-pentakisphosphate (triethylammonium salt)), molecular docking calculations, overview Arabidopsis thaliana
purpurogallin determination of ternary structure of AtIP5 2-K with purpurogallin and ADP, overview Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate Arabidopsis thaliana
-
ADP + 1D-myo-inositol hexakisphosphate
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q93YN9
-
-

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
Arabidopsis thaliana ADP + 1D-myo-inositol hexakisphosphate
-
?
additional information phosphotransfer from 2-FAM-InsP5 (2-O-(2-(5-fluoresceinylcarboxy)aminoethyl)-myo-inositol 1,3,4,5,6-pentakisphosphate) to ADP by AtIP5 2-K Arabidopsis thaliana ?
-
-

Synonyms

Synonyms Comment Organism
AtIP5 2-K
-
Arabidopsis thaliana
inositol pentakisphosphate 2-kinase
-
Arabidopsis thaliana
IP5 2-K
-
Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
assay at Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
ATP
-
Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction disruption of inositol pentakisphosphate 2-kinase profoundly influences cellular processes Arabidopsis thaliana
physiological function inositol pentakisphosphate 2-kinase catalyzes the phosphorylation of the axial 2-OH of myo-inositol 1,3,4,5,6-pentakisphosphate for de novo synthesis of myo-inositol hexakisphosphate Arabidopsis thaliana