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Literature summary for 2.7.1.158 extracted from

  • Gosein, V.; Miller, G.J.
    Conformational stability of inositol 1,3,4,5,6-pentakisphosphate 2-kinase (IPK1) dictates its substrate selectivity (2013), J. Biol. Chem., 288, 36788-36795.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
E82C/S142C the mutant exhibits a dithiothreitol-sensitive 5fold increase in kcat for 3,4,5,6-inositol tetrakisphosphate compared to the wild type enzyme Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04453
-
1D-myo-inositol 1,3,4,5,6-pentakisphosphate mutant enzyme E82C/S142C, at pH 7.5 and 25°C Arabidopsis thaliana
0.04754
-
1D-myo-inositol 3,4,5,6-tetrakisphosphate wild type enzyme, at pH 7.5 and 25°C Arabidopsis thaliana
0.06305
-
1D-myo-inositol 1,3,4,5,6-pentakisphosphate wild type enzyme, at pH 7.5 and 25°C Arabidopsis thaliana
0.07917
-
1D-myo-inositol 3,4,5,6-tetrakisphosphate mutant enzyme E82C/S142C, at pH 7.5 and 25°C Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate Arabidopsis thaliana
-
ADP + 1D-myo-inositol hexakisphosphate
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
Arabidopsis thaliana ADP + 1D-myo-inositol hexakisphosphate
-
?
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate low activity Arabidopsis thaliana ?
-
?

Synonyms

Synonyms Comment Organism
inositol 1,3,4,5,6-pentakisphosphate 2-kinase
-
Arabidopsis thaliana
Ipk1
-
Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.105
-
1D-myo-inositol 3,4,5,6-tetrakisphosphate wild type enzyme, at pH 7.5 and 25°C Arabidopsis thaliana
0.549
-
1D-myo-inositol 3,4,5,6-tetrakisphosphate mutant enzyme E82C/S142C, at pH 7.5 and 25°C Arabidopsis thaliana
0.734
-
1D-myo-inositol 1,3,4,5,6-pentakisphosphate wild type enzyme, at pH 7.5 and 25°C Arabidopsis thaliana
0.736
-
1D-myo-inositol 1,3,4,5,6-pentakisphosphate mutant enzyme E82C/S142C, at pH 7.5 and 25°C Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.2
-
1D-myo-inositol 3,4,5,6-tetrakisphosphate wild type enzyme, at pH 7.5 and 25°C Arabidopsis thaliana
6.8
-
1D-myo-inositol 3,4,5,6-tetrakisphosphate mutant enzyme E82C/S142C, at pH 7.5 and 25°C Arabidopsis thaliana
11.7
-
1D-myo-inositol 1,3,4,5,6-pentakisphosphate wild type enzyme, at pH 7.5 and 25°C Arabidopsis thaliana
16.5
-
1D-myo-inositol 1,3,4,5,6-pentakisphosphate mutant enzyme E82C/S142C, at pH 7.5 and 25°C Arabidopsis thaliana