Cloned (Comment) | Organism |
---|---|
cloning and expression in Escherichia coli | Zea mays |
expression in Escherichia coli | Zea mays |
General Stability | Organism |
---|---|
accumulation of soluble ZmIPK1 protein is very low under various growing temperatures and induction conditions, and the majority of the fusion protein is detected in inclusion bodies. Therefore, an alternative strategy using chaperone protein coexpression to enhance ZmIPK1 protein solubility is exploited. | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.119 | - |
1D-myo-inositol 1,3,4,5,6-pentakisphosphate | - |
Zea mays | |
0.119 | - |
myo-inositol 1,3,4,5,6-pentakisphosphate | - |
Zea mays | |
0.119 | - |
1D-myo-inositol 1,3,4,5,6-pentakisphosphate | 400 microM of substrate ATP is used | Zea mays |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
inclusion body | The majority of fusion protein is detected. | Zea mays | 16234 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48850 | - |
predicted | Zea mays |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1D-myo-inositol 1,3,4,5,6-pentakisphosphate + ATP | Zea mays | intermediate in both lipid-dependent and lipid-independent phytic acid biosynthetic pathways | 1D-myo-inositol hexakisphosphate + ADP | phytic acid, in higher plants, phytic acid can mediate abscisic acid-induced guard cell closure by inactivating plasma membrane inward K+ conductance. During this process, phytic acid appears to act as an endomembrane calcium-releasing signal. | ir | |
1D-myo-inositol 1,4,6-trisphosphate + ATP | Zea mays | relative substrate specificity 93.9% | 1D-myo-inositol 1,2,4,6-tetrakisphosphate + ADP | - |
? | |
additional information | Zea mays | capable to catalyze the phosphorylation of myo-inositol 1,4,5,6-tetrakisphosphate (relative substrate specificity 76.8%), and myo-inositol 3,4,5,6-tetrakisphosphate (relative substrate specificity 32.6%) | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
- |
- |
Zea mays | A4GWX8 | - |
- |
Zea mays | A4GWX8 | Alignments of the leaf- and seed-derived cDNA sequences with ZmIPK1A define nine predicted exons and eight predicted introns in theZmIPK1 genomic sequence | - |
Zea mays | A6YH13 | - |
- |
Zea mays | A6YH13 | contains a 31 bp deletion and a 7 bp polymorphism when compared to ZmIPK1A | - |
Purification (Comment) | Organism |
---|---|
a glutathione affinity column is used to capture intact GST-ZmIPK1. The purified GST-ZmIPK1 fusion is cleaved with thrombin and further purified by a sequential affinity and ion-exchange chromatography. ZmIPK1 protein is eluted at 0.1 M NaCl on a high resolution Mono Q column with an estimated purity of 95%. Identity of the purified protein is confirmed via trypsin digestion followed by matrix-assisted laser deposition/ionization (MALDI) time-of-flight mass spectrometry analysis and comparison of the MALDI fingerprint to the amino acid sequences deduced from ZmIPK1. | Zea mays |
expression in Escherichia coli | Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cob | immature ear | Zea mays | - |
corn silk | - |
Zea mays | - |
embryo | mature | Zea mays | - |
embryo | maturing, ZmIPK1A | Zea mays | - |
endosperm | midstage, ZmIPK1A | Zea mays | - |
endosperm | midstage | Zea mays | - |
leaf | - |
Zea mays | - |
leaf | a variety of alternative splicing products of ZmIPK1A are discovered in maize leaves and seeds | Zea mays | - |
leaf | A variety of alternative splicing products of ZmIPK1A are discovered in maize leaves and seeds. The products are derived from alternative acceptor sites, alternative donor sites, and retained introns in the transcripts. Up to 50% of the ZmIPK1A transcripts in maize seeds and leaves have an interrupted open reading frame. One type of splicing product of ZmIPK1B is detected in roots. | Zea mays | - |
additional information | A variety of alternative splicing products of ZmIPK1A are discovered in maize leaves and seeds. Products are derived from alternative acceptor sites, alternative donor sites, and retained introns in the transcripts. Up to 50% of the ZmIPK1A transcripts in maize seeds and leaves have an interrupted open reading frame. | Zea mays | - |
root | - |
Zea mays | - |
root | transcripts of ZmIPK1B were exclusively detected in roots | Zea mays | - |
root | transcripts detected in ZmIPK1B | Zea mays | - |
seed | - |
Zea mays | - |
seed | a variety of alternative splicing products of ZmIPK1A are discovered in maize leaves and seeds, ZmIPK1A | Zea mays | - |
seed | at 12 d after pollination | Zea mays | - |
silk | ZmIPK1A | Zea mays | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.625 | - |
- |
Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1D-myo-inositol 1,3,4,5,6-pentakisphosphate + ATP | intermediate in both lipid-dependent and lipid-independent phytic acid biosynthetic pathways | Zea mays | 1D-myo-inositol hexakisphosphate + ADP | phytic acid, in higher plants, phytic acid can mediate abscisic acid-induced guard cell closure by inactivating plasma membrane inward K+ conductance. During this process, phytic acid appears to act as an endomembrane calcium-releasing signal. | ir | |
1D-myo-inositol 1,4,6-trisphosphate + ATP | relative substrate specificity 93.9% | Zea mays | 1D-myo-inositol 1,2,4,6-tetrakisphosphate + ADP | - |
? | |
ATP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate | - |
Zea mays | ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate | - |
? | |
ATP + 1D-myo-inositol 3,4,5,6-tetrakisphosphate | - |
Zea mays | ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate | - |
? | |
ATP + myo-inositol 1,3,4,5,6-pentakisphosphate | - |
Zea mays | ADP + myo-inositol hexakisphosphate | - |
? | |
ATP + myo-inositol 1,4,6-trisphosphate | - |
Zea mays | ADP + inositol 1,2,4,6-tetrakisphosphate | - |
? | |
additional information | capable to catalyze the phosphorylation of myo-inositol 1,4,5,6-tetrakisphosphate (relative substrate specificity 76.8%), and myo-inositol 3,4,5,6-tetrakisphosphate (relative substrate specificity 32.6%) | Zea mays | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
inositol 1,3,4,5,6-pentakisphosphate 2-kinase | - |
Zea mays |
Ipk1 | - |
Zea mays |
ZmIPK1 | - |
Zea mays |
ZmIPK1A | - |
Zea mays |
ZmIPK1A | nearly identical paralog | Zea mays |
ZmIPK1A | nearly identical paralog to ZmIPK1, genomic ZmIPK1A sequence contains only one EcoRI site | Zea mays |
ZmIPK1B | - |
Zea mays |
ZmIPK1B | nearly identical paralog | Zea mays |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Zea mays |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Zea mays |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Zea mays | predicted | - |
7.8 |