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show all sequences of 2.7.1.157

Mechanistic studies on human N-acetylgalactosamine kinase

Agnew, A.; Timson, D.; J. Enzyme Inhib. Med. Chem. 25, 370-376 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli; GALK2, functional expression in Escherichia coli
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
alpha-methylgalactopyranoside
uncompetitive inhibition with respect to ATP; uncompetitive with respect to ATP, poor inhibitor
Homo sapiens
N-acetyl-D-galactosamine
substrate inhibition
Homo sapiens
N-acetylgalactosamine
substrate inhibition
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.014
-
ATP
pH 8.0, 37°C; recombinant enzyme
Homo sapiens
0.04
-
N-acetyl-alpha-D-galactosamine
pH 8.0, 37°C
Homo sapiens
0.04
-
N-acetyl-D-galactosamine
recombinant enzyme
Homo sapiens
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + N-acetyl-D-galactosamine
Homo sapiens
-
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Homo sapiens
Q01415
isoform GALK2
-
Purification (Commentary)
Commentary
Organism
recombinant GALK2 from Escherichia coli
Homo sapiens
Reaction
Reaction
Commentary
Organism
ATP + N-acetyl-alpha-D-galactosamine = ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
ordered ternary complex reaction mechanism, in which ATP binds first, overview. Ionizable residues play a role in substrate binding and catalysis
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + N-acetyl-alpha-D-galactosamine
-
709300
Homo sapiens
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
ATP + N-acetyl-D-galactosamine
-
709300
Homo sapiens
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
additional information
ordered ternary complex mechanism in which ATP is the first substrate to bind
709300
Homo sapiens
?
-
-
-
-
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1
-
ATP
recombinant enzyme
Homo sapiens
1
-
N-acetyl-alpha-D-galactosamine
pH 8.0, 37°C
Homo sapiens
1
-
N-acetyl-D-galactosamine
recombinant enzyme
Homo sapiens
pH Range
pH Minimum
pH Maximum
Commentary
Organism
additional information
-
pH dependency of the reaction mechanism, overview
Homo sapiens
7
8
-
Homo sapiens
Cofactor
Cofactor
Commentary
Organism
Structure
ATP
-
Homo sapiens
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
13
-
N-acetylgalactosamine
pH 8.0, 37°C
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Homo sapiens
GALK2, functional expression in Escherichia coli
Homo sapiens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
ATP
-
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
alpha-methylgalactopyranoside
uncompetitive inhibition with respect to ATP
Homo sapiens
alpha-methylgalactopyranoside
uncompetitive with respect to ATP, poor inhibitor
Homo sapiens
N-acetyl-D-galactosamine
substrate inhibition
Homo sapiens
N-acetylgalactosamine
substrate inhibition
Homo sapiens
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
13
-
N-acetylgalactosamine
pH 8.0, 37°C
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.014
-
ATP
recombinant enzyme
Homo sapiens
0.014
-
ATP
pH 8.0, 37°C
Homo sapiens
0.04
-
N-acetyl-alpha-D-galactosamine
pH 8.0, 37°C
Homo sapiens
0.04
-
N-acetyl-D-galactosamine
recombinant enzyme
Homo sapiens
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
-
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ATP + N-acetyl-D-galactosamine
Homo sapiens
-
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant GALK2 from Escherichia coli
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + N-acetyl-alpha-D-galactosamine
-
709300
Homo sapiens
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
ATP + N-acetyl-D-galactosamine
-
709300
Homo sapiens
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
additional information
ordered ternary complex mechanism in which ATP is the first substrate to bind
709300
Homo sapiens
?
-
-
-
-
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1
-
ATP
recombinant enzyme
Homo sapiens
1
-
N-acetyl-alpha-D-galactosamine
pH 8.0, 37°C
Homo sapiens
1
-
N-acetyl-D-galactosamine
recombinant enzyme
Homo sapiens
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
additional information
-
pH dependency of the reaction mechanism, overview
Homo sapiens
7
8
-
Homo sapiens
Other publictions for EC 2.7.1.157
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721309
Kristiansson
N-acetylgalactosamine kinase: ...
Homo sapiens
Appl. Biochem. Biotechnol.
166
57-63
2012
-
-
1
-
7
-
-
36
-
-
-
-
-
2
-
-
-
-
-
-
-
-
3
-
-
-
-
21
-
-
-
-
-
-
-
-
-
1
-
-
7
-
-
-
-
36
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
21
-
-
-
-
-
-
-
-
-
-
704525
Stockbridge
The intrinsic reactivity of AT ...
Homo sapiens
J. Biol. Chem.
284
22747-22757
2009
-
-
-
-
-
-
-
3
-
1
-
1
-
2
-
-
-
1
-
-
-
-
1
-
1
-
-
2
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
3
-
1
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
2
1
1
-
-
-
-
-
-
-
-
709300
Agnew
Mechanistic studies on human N ...
Homo sapiens
J. Enzyme Inhib. Med. Chem.
25
370-376
2009
-
-
1
-
-
-
3
3
-
1
-
1
-
4
-
-
1
1
-
-
-
-
3
-
-
-
-
3
-
2
-
1
1
-
-
-
-
2
1
-
-
-
-
4
1
4
-
1
-
1
-
-
-
1
-
-
-
-
3
-
-
-
-
3
-
2
-
-
-
-
-
-
-
-
659494
Thoden
The molecular architecture of ...
Homo sapiens
J. Biol. Chem.
280
32784-32791
2005
-
-
1
1
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
672583
Bourgeaux
Two-step enzymatic synthesis o ...
Homo sapiens
Bioorg. Med. Chem. Lett.
15
5459-5462
2005
-
1
1
-
-
-
-
1
-
-
1
-
-
3
-
-
1
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
1
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659129
Pastuszak
Kidney N-acetylgalactosamine ( ...
Homo sapiens, Oryctolagus cuniculus, Sus scrofa
J. Biol. Chem.
271
20776-20782
1996
-
-
-
-
-
-
-
2
1
3
1
-
-
5
-
-
1
-
-
8
1
-
7
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
3
1
-
-
-
-
1
-
8
1
-
7
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
659130
Pastuszak
Identification of the GalNAc k ...
Homo sapiens, Sus scrofa
J. Biol. Chem.
271
23653-23656
1996
-
-
1
-
-
-
-
2
2
-
1
-
-
5
-
-
-
-
-
2
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
2
-
1
-
-
-
-
-
-
2
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-