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show all sequences of 2.7.1.157

Two-step enzymatic synthesis of UDP-N-acetylgalactosamine

Bourgeaux, V.; Piller, F.; Piller, V.; Bioorg. Med. Chem. Lett. 15, 5459-5462 (2005)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
synthesis of UDP-GalNAc with high yield from GalNAc, UTP and ATP using recombinant human GalNAc kinase GK2 and UDP-GalNAc pyrophosphorylase AGX1
Homo sapiens
Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
-
Homo sapiens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
57000
-
x * 57000, SDS-PAGE
Homo sapiens
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
recombinant
-
Purification (Commentary)
Commentary
Organism
-
Homo sapiens
Storage Stability
Storage Stability
Organism
-20C, 0.5 mg/ml in a buffered (pH 7.5) solution containing 30% glycerol, no loss of activity after one year
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + N-acetyl-D-galactosamine
-
672583
Homo sapiens
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 57000, SDS-PAGE
Homo sapiens
Application (protein specific)
Application
Commentary
Organism
synthesis
synthesis of UDP-GalNAc with high yield from GalNAc, UTP and ATP using recombinant human GalNAc kinase GK2 and UDP-GalNAc pyrophosphorylase AGX1
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
-
Homo sapiens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
57000
-
x * 57000, SDS-PAGE
Homo sapiens
Purification (Commentary) (protein specific)
Commentary
Organism
-
Homo sapiens
Storage Stability (protein specific)
Storage Stability
Organism
-20C, 0.5 mg/ml in a buffered (pH 7.5) solution containing 30% glycerol, no loss of activity after one year
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + N-acetyl-D-galactosamine
-
672583
Homo sapiens
ADP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 57000, SDS-PAGE
Homo sapiens
Other publictions for EC 2.7.1.157
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721309
Kristiansson
N-acetylgalactosamine kinase: ...
Homo sapiens
Appl. Biochem. Biotechnol.
166
57-63
2012
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1
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7
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36
-
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2
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3
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21
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1
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7
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36
-
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3
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21
-
-
-
-
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704525
Stockbridge
The intrinsic reactivity of AT ...
Homo sapiens
J. Biol. Chem.
284
22747-22757
2009
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3
-
1
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1
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2
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1
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1
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1
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2
1
1
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1
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1
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3
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1
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1
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1
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1
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2
1
1
-
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709300
Agnew
Mechanistic studies on human N ...
Homo sapiens
J. Enzyme Inhib. Med. Chem.
25
370-376
2009
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1
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3
3
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1
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1
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4
-
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1
1
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3
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3
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2
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1
1
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2
1
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4
1
4
-
1
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1
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1
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3
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3
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2
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659494
Thoden
The molecular architecture of ...
Homo sapiens
J. Biol. Chem.
280
32784-32791
2005
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1
1
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1
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2
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1
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2
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1
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1
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1
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1
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2
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-
-
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-
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672583
Bourgeaux
Two-step enzymatic synthesis o ...
Homo sapiens
Bioorg. Med. Chem. Lett.
15
5459-5462
2005
-
1
1
-
-
-
-
1
-
-
1
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3
-
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1
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1
1
1
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1
1
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1
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1
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1
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1
1
1
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659129
Pastuszak
Kidney N-acetylgalactosamine ( ...
Homo sapiens, Oryctolagus cuniculus, Sus scrofa
J. Biol. Chem.
271
20776-20782
1996
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-
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2
1
3
1
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5
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1
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8
1
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7
1
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1
1
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1
3
1
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1
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8
1
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7
1
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1
1
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659130
Pastuszak
Identification of the GalNAc k ...
Homo sapiens, Sus scrofa
J. Biol. Chem.
271
23653-23656
1996
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1
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2
2
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1
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5
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2
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4
1
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1
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2
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1
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4
1
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