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Literature summary for 2.7.1.156 extracted from

  • Otte, M.M.; Woodson, J.D.; Escalante-Semerena, J.C.
    The thiamine kinase (YcfN) enzyme plays a minor but significant role in cobinamide salvaging in Salmonella enterica (2007), J. Bacteriol., 189, 7310-7315.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
A chromosomal in-frame deletion of the cobU gene is constructed in strain TR6583. Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
adenosylcobinamide + ATP Salmonella enterica subsp. enterica serovar Typhimurium
-
adenosylcobinamide phosphate + ADP
-
ir
adenosylcobinamide + GTP Salmonella enterica subsp. enterica serovar Typhimurium
-
adenosylcobinamide phosphate + GDP
-
ir
adenosylcobinamide phosphate + GTP Salmonella enterica subsp. enterica serovar Typhimurium
-
adenosylcobinamide-GDP + diphosphate
-
ir
additional information Salmonella enterica subsp. enterica serovar Typhimurium CobU enzyme of the nucleotide loop assembly pathway is a bifunctional enzyme with adenosylcobinamide and adenosylcobinamide-phosphate guanylyltransferase activity, important for de novo synthesis and for cobinamide salvaging ?
-
?

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
Typhimurium LT2
-

Purification (Commentary)

Purification (Comment) Organism
CobU protein is overproduced and purified. Cells from a 4-liter culture of the overproducing strain are lysed using a French press. After the addition of ammonium sulfate, fast protein liquid chromatography is used to isolate CobU protein from the clarified cell extract in two additional steps, hydrophobic interaction chromatography and dye ligand chromatography. Protein concentration was determined by the Bradford method. Salmonella enterica subsp. enterica serovar Typhimurium

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Salmonella enterica subsp. enterica serovar Typhimurium
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
adenosylcobinamide + ATP
-
Salmonella enterica subsp. enterica serovar Typhimurium adenosylcobinamide phosphate + ADP
-
ir
adenosylcobinamide + GTP
-
Salmonella enterica subsp. enterica serovar Typhimurium adenosylcobinamide phosphate + GDP
-
ir
adenosylcobinamide phosphate + GTP
-
Salmonella enterica subsp. enterica serovar Typhimurium adenosylcobinamide-GDP + diphosphate
-
ir
additional information CobU enzyme of the nucleotide loop assembly pathway is a bifunctional enzyme with adenosylcobinamide and adenosylcobinamide-phosphate guanylyltransferase activity, important for de novo synthesis and for cobinamide salvaging Salmonella enterica subsp. enterica serovar Typhimurium ?
-
?

Synonyms

Synonyms Comment Organism
ATP/GTP:AdoCbi kinase
-
Salmonella enterica subsp. enterica serovar Typhimurium
CobU
-
Salmonella enterica subsp. enterica serovar Typhimurium
GTP:adenosylcobinamide-phosphate guanylyltransferase
-
Salmonella enterica subsp. enterica serovar Typhimurium
GTP:AdoCbi-P kinase
-
Salmonella enterica subsp. enterica serovar Typhimurium
GTP:AdoCbi-phosphate guanylyltransferase
-
Salmonella enterica subsp. enterica serovar Typhimurium
NTP:adenosylcobinamide kinase
-
Salmonella enterica subsp. enterica serovar Typhimurium
NTP:AdoCbi kinase
-
Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

Cofactor Comment Organism Structure
ATP
-
Salmonella enterica subsp. enterica serovar Typhimurium
GTP
-
Salmonella enterica subsp. enterica serovar Typhimurium