Cloned (Comment) | Organism |
---|---|
A chromosomal in-frame deletion of the cobU gene is constructed in strain TR6583. | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenosylcobinamide + ATP | Salmonella enterica subsp. enterica serovar Typhimurium | - |
adenosylcobinamide phosphate + ADP | - |
ir | |
adenosylcobinamide + GTP | Salmonella enterica subsp. enterica serovar Typhimurium | - |
adenosylcobinamide phosphate + GDP | - |
ir | |
adenosylcobinamide phosphate + GTP | Salmonella enterica subsp. enterica serovar Typhimurium | - |
adenosylcobinamide-GDP + diphosphate | - |
ir | |
additional information | Salmonella enterica subsp. enterica serovar Typhimurium | CobU enzyme of the nucleotide loop assembly pathway is a bifunctional enzyme with adenosylcobinamide and adenosylcobinamide-phosphate guanylyltransferase activity, important for de novo synthesis and for cobinamide salvaging | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
Typhimurium LT2 | - |
Purification (Comment) | Organism |
---|---|
CobU protein is overproduced and purified. Cells from a 4-liter culture of the overproducing strain are lysed using a French press. After the addition of ammonium sulfate, fast protein liquid chromatography is used to isolate CobU protein from the clarified cell extract in two additional steps, hydrophobic interaction chromatography and dye ligand chromatography. Protein concentration was determined by the Bradford method. | Salmonella enterica subsp. enterica serovar Typhimurium |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenosylcobinamide + ATP | - |
Salmonella enterica subsp. enterica serovar Typhimurium | adenosylcobinamide phosphate + ADP | - |
ir | |
adenosylcobinamide + GTP | - |
Salmonella enterica subsp. enterica serovar Typhimurium | adenosylcobinamide phosphate + GDP | - |
ir | |
adenosylcobinamide phosphate + GTP | - |
Salmonella enterica subsp. enterica serovar Typhimurium | adenosylcobinamide-GDP + diphosphate | - |
ir | |
additional information | CobU enzyme of the nucleotide loop assembly pathway is a bifunctional enzyme with adenosylcobinamide and adenosylcobinamide-phosphate guanylyltransferase activity, important for de novo synthesis and for cobinamide salvaging | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ATP/GTP:AdoCbi kinase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
CobU | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
GTP:adenosylcobinamide-phosphate guanylyltransferase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
GTP:AdoCbi-P kinase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
GTP:AdoCbi-phosphate guanylyltransferase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
NTP:adenosylcobinamide kinase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
NTP:AdoCbi kinase | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
GTP | - |
Salmonella enterica subsp. enterica serovar Typhimurium |