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Literature summary for 2.7.1.154 extracted from

  • Mountford, J.K.; Petitjean, C.; Putra, H.W.; McCafferty, J.A.; Setiabakti, N.M.; Lee, H.; T?nnesen, L.L.; McFadyen, J.D.; Schoenwaelder, S.M.; Eckly, A.; Gachet, C.; Ellis, S.; Voss, A.K.; Dickins, R.A.; Hamilton, J.R.; Jackson, S.P.
    The class II PI 3-kinase, PI3KC2alpha, links platelet internal membrane structure to shear-dependent adhesive function (2015), Nat. Commun., 6, 6535.
    View publication on PubMed
Search for more literature for 2.7.1.154

Cloned(Commentary)

Cloned (Comment) Organism
gene Pik3c2alpha, expression analysis Mus musculus

Protein Variants

Protein Variants Comment Organism
additional information disruption of gene Pik3c2alpha. Insertion of a gene trap vector encoding a splice acceptor beta-galactosidase (trap) and a polyA stop site into the Pik3c2alpha locus occur either between exons 19 and 20 (Pik3c2alphagt1) or exons 23 and 24 (Pik3c2alphagt2), and is predicted to yield a truncated fusion protein of the first 837 or 1029 amino acids of PI3KC2alpha-linked to beta-galactosidase, respectively. shRNA expression using the cytomegalovirus (CMV) promoter Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Mus musculus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate Mus musculus
-
 ADP + 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus Q61194 gene Pik3c2alpha
-

Source Tissue

Source Tissue Comment Organism Textmining
blood platelet
-
 Mus musculus
-
megakaryocyte
-
 Mus musculus
-
additional information isozyme PI3KC2alpha is widely expressed in mammalian cells Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol 4-phosphate
-
 Mus musculus ADP + 1-phosphatidyl-1D-myo-inositol 3,4-bisphosphate
-
 ?

Synonyms

Synonyms Comment Organism
class II PI3K
-
 Mus musculus
phosphoinositide 3-kinases
-
 Mus musculus
PI3KC2alpha
-
 Mus musculus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Mus musculus

General Information

General Information Comment Organism
malfunction agonist-induced 3-phosphorylated phosphoinositide production and cellular activation are normal in PI3KC2alpha-deficient platelets. PI3KC2alpha deficiency exacerbates bleeding and impairs thrombosis. Phenotype of Pik3c2alpha-/- mice. PI3KC2alpha deficiency alters platelet and megakaryocyte internal membrane structures, overview. PI3KC2alpha deficiency causes unstable platelet thrombi and enhances shear-dependent platelet adhesion Mus musculus
physiological function PI3KC2alpha is important for mouse platelet function in vivo and plays an important role in regulating endothelial cell barrier function and vasculogenesis. Role for PI3KC2alpha in regulating the internal membrane reserve structure of megakaryocytes (demarcation membrane system) and platelets (open canalicular system) that results in dysregulated platelet adhesion under haemodynamic shear stress. Important role for PI3KC2alpha in regulating shear-dependent platelet adhesion via regulation of membrane structure, rather than acute signalling Mus musculus