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Literature summary for 2.7.1.153 extracted from

  • Chamberlain, M.D.; Chan, T.; Oberg, J.C.; Hawrysh, A.D.; James, K.M.; Saxena, A.; Xiang, J.; Anderson, D.H.
    Disrupted RabGAP function of the p85 subunit of phosphatidylinositol 3-kinase results in cell transformation (2008), J. Biol. Chem., 283, 15861-15868.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of HA-tagged p85alpha in NIH 3T3 cells in a mouse cell model Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information sequencing of cancer cell p85alpha subunits reveal four mutational alterations, three colonic and one ovarian: one with an early stop codon after Asp605 and three containing small in-frame deletions, DELTALeu570-Gln572, DELTALeu570-Asp578, and DELTAMet582-Asp605. These deletions are located near the negative regulatory phosphorylation site Ser608 within p85alpha. All mutations lead to increased enzyme activity Homo sapiens
R274A the GTPase-activating protein activity toward Rab5 and Rab4 of PI3K p85alpha subunit is abolished in the mutant. Expression of p85alpha-R274A results in increased platelet-derived growth factor receptor, PDGFR, activation and downstream signaling, via Akt and MAPK pathways, and in decreased PDGFR degradation. Disrupted RabGAP function of the p85 subunit of phosphatidylinositol 3-kinase results in cell transformation, co-expression of a dominant negative Rab5-S34N mutant attenuates these transformed properties Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the p85alpha subunit of phosphatidylinositol 3-kinase has GTPase-activating protein activity toward Rab5 and Rab4, small monomeric GTPases important in the regulation of RTK endocytosis, trafficking, and degradation pathways ?
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Organism

Organism UniProt Comment Textmining
Homo sapiens
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the p85alpha subunit of phosphatidylinositol 3-kinase has GTPase-activating protein activity toward Rab5 and Rab4, small monomeric GTPases important in the regulation of RTK endocytosis, trafficking, and degradation pathways Homo sapiens ?
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Synonyms

Synonyms Comment Organism
PI3K
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Homo sapiens