Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | Expression of AtIpk2b increases salt tolerance of transgenic tobacco plants | Arabidopsis thaliana | |
additional information | In ten-day-old seedlings grown on MS medium, AtIpk2b transcript level increases slightly upon exposure to cold or drought stress | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
Expression of AtIpk2b in yeast mutant arg82D, quantitative real-time PCR analysis of AtIpk2b expression in Arabidopsis. AtIpk2b cDNA containing the complete open reading frame is inserted via XbaI-SalI sites into the binary vector pBinAR-HPT. Resulting construct is introduced into Agrobacterium tumefaciens (strain LBA4404). Tobacco leaf disks are infected with the transformed Agrobacteria. | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
abscisic acid | In ten-day-old seedlings grown on MS medium, AtIpk2b transcript level decreased. | Arabidopsis thaliana | |
NaCl | In ten-day-old seedlings grown on MS medium, AtIpk2b transcript level decreases in response to salt | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
calculated and confirmed by Western Blot analysis | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
enzyme expressed in Saccharomyces cerevisiae and in Nicotiana tabacum. Transgenic plants show increased tolerance to osmotic, drought, freezing temperature and oxidative stress. | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | used for western blot analysis | Arabidopsis thaliana | - |
seedling | Ten-day-old Arabidopsis seedlings grown on MS medium are used for different treatments. RNA is extracted and reverse transcription of RNA is carried out using M-MLV RTase Synthesis Kit. | Arabidopsis thaliana | - |
Synonyms | Comment | Organism |
---|---|---|
AtIpk2beta | - |
Arabidopsis thaliana |
inositol 1,4,5-trisphosphate 3-kinase | - |
Arabidopsis thaliana |
inositol polyphosphate 6-/3-kinase | - |
Arabidopsis thaliana |
IP3K | - |
Arabidopsis thaliana |