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Literature summary for 2.7.1.151 extracted from

  • Yang, L.; Tang, R.; Zhu, J.; Liu, H.; Mueller-Roeber, B.; Xia, H.; Zhang, H.
    Enhancement of stress tolerance in transgenic tobacco plants constitutively expressing AtIpk2beta, an inositol polyphosphate 6-/3-kinase from Arabidopsis thaliana (2008), Plant Mol. Biol., 66, 329-343.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information Expression of AtIpk2b increases salt tolerance of transgenic tobacco plants Arabidopsis thaliana
additional information In ten-day-old seedlings grown on MS medium, AtIpk2b transcript level increases slightly upon exposure to cold or drought stress Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
Expression of AtIpk2b in yeast mutant arg82D, quantitative real-time PCR analysis of AtIpk2b expression in Arabidopsis. AtIpk2b cDNA containing the complete open reading frame is inserted via XbaI-SalI sites into the binary vector pBinAR-HPT. Resulting construct is introduced into Agrobacterium tumefaciens (strain LBA4404). Tobacco leaf disks are infected with the transformed Agrobacteria. Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
abscisic acid In ten-day-old seedlings grown on MS medium, AtIpk2b transcript level decreased. Arabidopsis thaliana
NaCl In ten-day-old seedlings grown on MS medium, AtIpk2b transcript level decreases in response to salt Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
calculated and confirmed by Western Blot analysis Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
enzyme expressed in Saccharomyces cerevisiae and in Nicotiana tabacum. Transgenic plants show increased tolerance to osmotic, drought, freezing temperature and oxidative stress.
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Source Tissue

Source Tissue Comment Organism Textmining
leaf used for western blot analysis Arabidopsis thaliana
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seedling Ten-day-old Arabidopsis seedlings grown on MS medium are used for different treatments. RNA is extracted and reverse transcription of RNA is carried out using M-MLV RTase Synthesis Kit. Arabidopsis thaliana
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Synonyms

Synonyms Comment Organism
AtIpk2beta
-
Arabidopsis thaliana
inositol 1,4,5-trisphosphate 3-kinase
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Arabidopsis thaliana
inositol polyphosphate 6-/3-kinase
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Arabidopsis thaliana
IP3K
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Arabidopsis thaliana