Cloned (Comment) | Organism |
---|---|
expression of wild-type enzyme and deletion mutant in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of an N-terminal deletion mutant comprising residues 266-371, the mutant enzyme is inhibited by aurintricarboxylic acid, gossypol, 3',4',7,8-tetrahydroxyflavone, epigallocatechin-3-gallate, chlorogenic acid, and rose bengal, but not by quercetin, epicatechin-3-gallate, ellagic acid, hypericin, and myricetin in contrary to the wild-type | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
aurintricarboxylic acid | mixed-type versus ATP, noncompetitive versus 1D-myo-inositol 1,4,5-trisphosphate, strong inhibition, IC50 is 44 nM | Homo sapiens | |
chlorogenic acid | mixed-type versus ATP, noncompetitive versus 1D-myo-inositol 1,4,5-trisphosphate, slight inhibition, IC50 is 0.0012 mM | Homo sapiens | |
ellagic acid | mixed-type versus ATP, noncompetitive versus 1D-myo-inositol 1,4,5-trisphosphate, slight inhibition, IC50 is 0.0014 mM | Homo sapiens | |
gossypol | mixed-type versus ATP, noncompetitive versus 1D-myo-inositol 1,4,5-trisphosphate, slight inhibition, IC50 is 0.0034 mM | Homo sapiens | |
additional information | inhibitory effects are not due to inactivating aggregation of the enzyme, no inhibition by quercetin, 3',4',7,8-tetrahydroxyflavone, myricetin, hypericin, epigallocatechin-3-gallate, and epicatechin-3-gallate, the enzyme contains a unique basic segment of 105 amino acids, which is inserted between the SSLL motif and the DFG motif, being probably involved in inhibition by acidic polyphenols | Homo sapiens | |
Rose bengal | mixed-type versus ATP, noncompetitive versus 1D-myo-inositol 1,4,5-trisphosphate, slight inhibition, IC50 is 620 nM | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00035 | - |
1D-myo-inositol 1,4,5-trisphosphate | recombinant wild-type enzyme, pH 7.5, 30°C | Homo sapiens | |
0.0004 | - |
1D-myo-inositol 1,4,5-trisphosphate | recombinant deletion mutant enzyme, pH 7.5, 30°C | Homo sapiens | |
0.017 | - |
ATP | recombinant wild-type enzyme, pH 7.5, 30°C | Homo sapiens | |
0.039 | - |
ATP | recombinant deletion mutant enzyme, pH 7.5, 30°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | the enzyme contains a unique basic segment of 105 amino acids, which is inserted between the SSLL motif and the DFG motif, being responsible for nuclear localization and is probably also involved in inhibition by acidic polyphenols | Homo sapiens | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens | |
additional information | free enzyme and inhibited enzyme are not affected by Ca2+ | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol 1,4,5-trisphosphate | Homo sapiens | - |
ADP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate | - |
? | |
additional information | Homo sapiens | the enzyme might be involved in regulation of cell growth | ? | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
additional information | enzyme is not affected by 3% v/v DMSO | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type enzyme and deletion mutant from Escherichia coli by ion exchange and phosphocellulose resin chromatography | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.5 | - |
recombinant wild-type enzyme, substrate 1D-myo-inositol 1,4,5-trisphosphate | Homo sapiens |
5.5 | - |
recombinant deletion mutant enzyme, substrate 1D-myo-inositol 1,4,5-trisphosphate | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1D-myo-inositol 1,4,5-trisphosphate | - |
Homo sapiens | ADP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate | - |
? | |
additional information | the enzyme might be involved in regulation of cell growth | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
inositol polyphosphate multikinase | - |
Homo sapiens |
IPMK | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000044 | - |
mixed-type versus ATP, noncompetitive versus 1D-myo-inositol 1,4,5-trisphosphate, strong inhibition, IC50 is 44 nM | Homo sapiens | aurintricarboxylic acid | |
0.00062 | - |
mixed-type versus ATP, noncompetitive versus 1D-myo-inositol 1,4,5-trisphosphate, slight inhibition, IC50 is 620 nM | Homo sapiens | Rose bengal | |
0.0012 | - |
mixed-type versus ATP, noncompetitive versus 1D-myo-inositol 1,4,5-trisphosphate, slight inhibition, IC50 is 0.0012 mM | Homo sapiens | chlorogenic acid | |
0.0014 | - |
mixed-type versus ATP, noncompetitive versus 1D-myo-inositol 1,4,5-trisphosphate, slight inhibition, IC50 is 0.0014 mM | Homo sapiens | ellagic acid | |
0.0034 | - |
mixed-type versus ATP, noncompetitive versus 1D-myo-inositol 1,4,5-trisphosphate, slight inhibition, IC50 is 0.0034 mM | Homo sapiens | gossypol |