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Literature summary for 2.7.1.151 extracted from

  • Nalaskowski, M.M.; Deschermeier, C.; Fanick, W.; Mayr, G.W.
    The human homologue of yeast ArgRIII protein is an inositol phosphate multikinase with predominantly nuclear localization (2002), Biochem. J., 366, 549-556.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.151

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, overexpression as C-terminally GFP-tagged fusion protein in Escherichia coli strain BL21(DE3), transient expression in NRK 52E cells as C- or N-terminally GFP-tagged fusion protein Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00028
-
 1D-myo-inositol 1,4,5-trisphosphate pH 7.8, 30°C, 0.5 mM ATP, recombinant enzyme Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus predominantly, a basic cluster at the enzyme's C-terminus is involved in nuclear targeting Homo sapiens 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
KCl
-
 Homo sapiens
Mg2+
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47219
-
 x * 47219, amino acid sequence calculation Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8NFU5
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL21(DE3) to near homogeneity by ion exchange and phosphocellulose resin chromatography Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
ATP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate = ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate the catalytic site contains an important Ser-Ser-Leu-Leu motif Homo sapiens
a
ATP + 1D-myo-inositol 1,4,5-trisphosphate = ADP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate the catalytic site contains an important Ser-Ser-Leu-Leu motif Homo sapiens
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.45
-
 purified recombinant enzyme, 1D-myo-inositol 1,3,4,5,6-pentakisphosphate formation from 1D-myo-inositol 1,4,5-trisphosphate, 0.5 mM ATP Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ATP + 1D-myo-inositol 1,4,5-trisphosphate
-
 Homo sapiens 2 ADP + 1D-myo-inositol 1,3,4,5,6-pentakisphosphate
-
 ?
ATP + 1D-myo-inositol 1,4,5-trisphosphate
-
 Homo sapiens ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate
-
 ir
ATP + 1D-myo-inositol 1,4,5-trisphosphate
-
 Homo sapiens ADP + 1D-myo-inositol 1,4,5,6-tetrakisphosphate
-
 ir

Subunits

Subunits Comment Organism
? x * 47219, amino acid sequence calculation Homo sapiens

Synonyms

Synonyms Comment Organism
inositol phosphate multikinase
-
 Homo sapiens
IPMK
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens