Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.150 extracted from

  • Bhakt, P.; Shivarathri, R.; Choudhary, D.K.; Borah, S.; Kaur, R.
    Fluconazole-induced actin cytoskeleton remodeling requires phosphatidylinositol 3-phosphate 5-kinase in the pathogenic yeast Candida glabrata (2018), Mol. Microbiol., 110, 425-443 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.150

Cloned(Commentary)

Cloned (Comment) Organism
gene CgFAB1, quantitative real-time PCR expression analysis, recombinant expression of C-terminally GFP-tagged enzyme [Candida] glabrata

Protein Variants

Protein Variants Comment Organism
G1867/1870V site-directed mutagenesis, the Cgfab1DELTA mutant carrying CgFab1G1867/1870V is sensitive to both azole and metal ion stress [Candida] glabrata
additional information generation of a deletion strain for the CgFAB1 gene, using the homologous recombination-based strategy. The mutant is sensitive to azoles (fluconazole, clotrimazole and ketoconazole), and cell membrane (SDS) and cell wall (caffeine) stressors. The growth of the Cgfab1DELTA mutant is also slightly impaired in the presence of the oxidative stressor, hydrogen peroxide, and at high temperature. MDR genes are activated in the Cgfab1DELTA mutant upon fluconazole exposure [Candida] glabrata

Localization

Localization Comment Organism GeneOntology No. Textmining
vacuolar membrane
-
 [Candida] glabrata 5774
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required [Candida] glabrata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol 3-phosphate [Candida] glabrata
-
 ADP + 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
[Candida] glabrata
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
-
 [Candida] glabrata ADP + 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphate
-
 ?

Synonyms

Synonyms Comment Organism
CgFab1
-
 [Candida] glabrata
phosphatidylinositol 3-phosphate 5-kinase
-
 [Candida] glabrata
PI3P-5 kinase
-
 [Candida] glabrata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at [Candida] glabrata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at [Candida] glabrata

Cofactor

Cofactor Comment Organism Structure
ATP
-
 [Candida] glabrata

General Information

General Information Comment Organism
malfunction CgFAB1 disruption impaires vacuole homeostasis and actin organization, and the F-actin stabilizing compound jasplakinolide rescues azole toxicity in cytoskeleton defective-mutants including the Cgfab1DELTA mutant. The actin depolymerization factor CgCof1 binds to multiple lipids including phosphatidylinositol 3,5-bisphosphate. CgCof1 distribution along with the actin filament-capping protein CgCap2 is altered upon both CgFAB1 disruption and fluconazole exposure. Actin polymerization inhibition renders fluconazole fully and partially fungicidal in azole-susceptible and azole-resistant Candida glabrata clinical isolates, respectively, thereby, underscoring the role of fluconazole-effectuated actin remodeling in azole resistance. MDR genes are activated in the Cgfab1DELTA mutant upon fluconazole exposure. Fluconazole-treated wild-type and Cgfab1DELTA cells display a 1.5fold increased efflux of the MDR pump substrate rhodamine 6G [Candida] glabrata
additional information Gly867 and Gly1870 are required for the functioning of CgFab1 [Candida] glabrata
physiological function enzyme CgFab1 is implicated in azole tolerance through actin network remodeling. CgFab1 is required for vacuole homeostasis. Fluconazole induces actin cytoskeletal depolarization in a CgFab1-dependent manner, in part, through the PI 3,5-bisphosphate [PI(3,5)P2]-binding protein, CgCof1. CgFab1 is involved in response to azole, cell membrane and cell wall stress. Fluconazole-treated wild-type and Cgfab1DELTA cells display a 1.5fold increased efflux of the MDR pump substrate rhodamine 6G [Candida] glabrata