BRENDA - Enzyme Database
show all sequences of 2.7.1.15

Molecular cloning, expression and characterization of ribokinase of Leishmania major

Ogbunude, P.O.; Lamour, N.; Barrett, M.P.; Acta Biochim. Biophys. Sin. 39, 462-466 (2007)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
overexpression in Escherichia coli
Leishmania major
Inhibitors
Inhibitors
Commentary
Organism
Structure
D-arabinose
-
Leishmania major
D-erythrose
-
Leishmania major
D-fructose
-
Leishmania major
D-glucose
-
Leishmania major
D-ribose 5-phosphate
inhibits the phosphorylation of D-ribose
Leishmania major
D-ribulose
-
Leishmania major
D-threose
-
Leishmania major
D-xylulose
-
Leishmania major
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
ATP
-
Leishmania major
0.3
-
D-ribose
-
Leishmania major
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Leishmania major
-
-
-
Storage Stability
Storage Stability
Organism
-70°C, in buffer/glycerol (1/1), the recombinant enzyme retains more than 50% of its activity after 1 year
Leishmania major
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + D-ribose
-
671029
Leishmania major
ADP + D-ribose 5-phosphate
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
10.2
-
ATP
22°C, pH 8.5
Leishmania major
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.4
-
D-ribose 5-phosphate
22°C, pH 8.5
Leishmania major
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpression in Escherichia coli
Leishmania major
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
D-arabinose
-
Leishmania major
D-erythrose
-
Leishmania major
D-fructose
-
Leishmania major
D-glucose
-
Leishmania major
D-ribose 5-phosphate
inhibits the phosphorylation of D-ribose
Leishmania major
D-ribulose
-
Leishmania major
D-threose
-
Leishmania major
D-xylulose
-
Leishmania major
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.4
-
D-ribose 5-phosphate
22°C, pH 8.5
Leishmania major
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.2
-
ATP
-
Leishmania major
0.3
-
D-ribose
-
Leishmania major
Storage Stability (protein specific)
Storage Stability
Organism
-70°C, in buffer/glycerol (1/1), the recombinant enzyme retains more than 50% of its activity after 1 year
Leishmania major
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ATP + D-ribose
-
671029
Leishmania major
ADP + D-ribose 5-phosphate
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
10.2
-
ATP
22°C, pH 8.5
Leishmania major
Other publictions for EC 2.7.1.15
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737810
Quiroga-Roger
Role of monovalent and divalen ...
Homo sapiens
Biometals
28
401-413
2015
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6
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5
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2
6
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1
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7
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7
7
737373
Paul
Crystallization and preliminar ...
Vibrio cholerae, Vibrio cholerae O395
Acta Crystallogr. Sect. F
70
1098-1102
2014
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1
1
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721227
Kori
Expression, purification, crys ...
Halothermothrix orenii
Acta Crystallogr. Sect. F
68
240-243
2012
-
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1
1
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1
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723067
Li
Crystal structure of Sa239 rev ...
Staphylococcus aureus
J. Struct. Biol.
177
578-582
2012
-
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1
1
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2
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1
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3
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1
1
1
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1
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1
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1
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2
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1
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-
1
1
1
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-
2
1
-
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2
2
723785
Stepchenko
-
Enzymatic synthesis of 2-deoxy ...
Escherichia coli
Synlett
23
1541-1545
2012
-
-
-
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1
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-
713968
Yang
Physical and functional intera ...
Mycobacterium tuberculosis, Mycolicibacterium smegmatis
Arch. Biochem. Biophys.
512
135-142
2011
2
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2
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3
-
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2
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5
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2
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4
-
2
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2
2
-
-
-
695385
Wang
Cloning, purification, crystal ...
Staphylococcus aureus
Acta Crystallogr. Sect. F
65
574-576
2009
-
-
1
1
-
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-
-
-
1
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1
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2
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1
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1
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1
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1
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1
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1
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1
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-
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1
1
-
-
-
686025
Park
Adenosine kinase and ribokinas ...
Arabidopsis thaliana, Bos taurus, Burkholderia cepacia, Entamoeba histolytica, Escherichia coli, Homo sapiens, Leishmania major, Saccharomyces cerevisiae, Salmonella enterica subsp. enterica serovar Typhimurium, Trypanosoma cruzi
Cell. Mol. Life Sci.
65
2875-2896
2008
1
-
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5
-
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10
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12
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1
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5
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12
-
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-
-
-
-
-
-
-
-
-
-
-
-
700031
Brinkrolf
The LacI/GalR family transcrip ...
Corynebacterium glutamicum, Corynebacterium glutamicum DSM 20300
Microbiology
154
1068-1081
2008
-
-
1
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-
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1
2
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5
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2
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1
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1
-
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671029
Ogbunude
Molecular cloning, expression ...
Leishmania major
Acta Biochim. Biophys. Sin.
39
462-466
2007
-
-
1
-
-
-
8
2
-
-
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-
-
3
-
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-
-
1
1
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-
-
1
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1
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1
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8
1
2
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1
1
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1
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-
-
-
673692
Park
Identification and characteriz ...
Escherichia coli, Homo sapiens
FEBS Lett.
581
3211-3216
2007
1
-
2
-
-
-
16
3
-
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4
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2
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5
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4
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1
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16
4
3
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2
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5
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673772
Ulanova
Mutation of a gene encoding a ...
Bacillus subtilis
Folia Microbiol. (Praha)
52
203-208
2007
-
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1
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4
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684118
Ogbunude
Molecular cloning, expression ...
Leishmania major
Acta Biochim. Biophys. Sin. (Shanghai)
39
462-466
2007
-
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1
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1
2
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3
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1
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1
1
2
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1
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2
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672549
Chuvikovsky
Ribokinase from E. coli: expre ...
Escherichia coli
Bioorg. Med. Chem.
14
6327-6332
2006
-
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1
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5
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3
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1
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1
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6
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641006
Andersson
Activation of ribokinase by mo ...
Escherichia coli
J. Mol. Biol.
315
409-419
2002
-
-
1
1
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2
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641005
Maj
The effect of inorganic phosph ...
Escherichia coli
J. Protein Chem.
20
139-144
2001
-
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2
13
-
1
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2
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641007
Tourneux
Genetic and biochemical charac ...
Salmonella enterica
J. Bacteriol.
182
869-873
2000
-
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1
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1
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2
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1
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4
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641004
Sigrell
Induced fit on sugar binding a ...
Escherichia coli
J. Mol. Biol.
290
1009-1018
1999
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1
1
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641003
Sigrell
Structure of Escherichia coli ...
Escherichia coli
Structure
6
183-193
1998
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641002
Sigrell
Purification, characterization ...
Escherichia coli
Protein Sci.
6
2474-2476
1997
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1
1
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1
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641001
Goitein
A rapid assay for ribokinase ...
Klebsiella aerogenes
Anal. Biochem.
75
660-663
1976
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1
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1
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641000
Schimmel
Deoxyribokinase from Salmonell ...
Salmonella enterica subsp. enterica serovar Typhimurium
Arch. Biochem. Biophys.
164
560-570
1974
-
-
-
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3
4
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11
2
1
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2
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1
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1
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10
1
1
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1
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3
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4
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11
2
1
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1
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1
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10
1
1
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1
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-
640998
Ginsburg
A deoxyribokinase from Lactoba ...
Lactobacillus plantarum
J. Biol. Chem.
234
481-487
1959
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5
1
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2
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1
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2
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1
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2
1
3
-
1
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1
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1
1
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5
-
1
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2
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640999
Agranoff
Purification and properties of ...
Bos taurus
J. Biol. Chem.
219
221-229
1956
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